The family II carbohydrate-binding module of xylanase CflXyn11A from Cellulomonas flavigena increases the synergy with cellulase TrCel7B from Trichoderma reesei during the hydrolysis of sugar cane bagasse.
(2012) In Bioresource Technology 104. p.622-630- Abstract
- Synergy between Cellulomonas flavigena xylanase CflXyn11A and Trichoderma reesei endoglucanase TrCel7B was assessed during hydrolysis of alkaline pretreated sugar cane bagasse (SCB) after 12-48h, applying the individual enzymes and mixtures of the enzymes. A high degree of synergy (6.3) between CflXyn11A and TrCel7B in hydrolysis of SCB was observed after 12h in the equimolar mixture. A threefold decrease in the degree of synergy was observed with TrCel7B and the catalytic module of CflXyn11A; suggesting an important role played by the carbohydrate-binding module of CflXyn11A (CflXyn11A-CBM) in the observed synergy. Affinity electrophoresis and binding assays showed that CflXyn11A-CBM binds to xylans and to a lesser extent to cellulose.... (More)
- Synergy between Cellulomonas flavigena xylanase CflXyn11A and Trichoderma reesei endoglucanase TrCel7B was assessed during hydrolysis of alkaline pretreated sugar cane bagasse (SCB) after 12-48h, applying the individual enzymes and mixtures of the enzymes. A high degree of synergy (6.3) between CflXyn11A and TrCel7B in hydrolysis of SCB was observed after 12h in the equimolar mixture. A threefold decrease in the degree of synergy was observed with TrCel7B and the catalytic module of CflXyn11A; suggesting an important role played by the carbohydrate-binding module of CflXyn11A (CflXyn11A-CBM) in the observed synergy. Affinity electrophoresis and binding assays showed that CflXyn11A-CBM binds to xylans and to a lesser extent to cellulose. Our results suggest that synergy is more pronounced at early stages of hydrolysis. Furthermore, for the first time it is described that a CBM carried by a xylanase significantly enhances the synergy with a cellulase (threefold increase in synergy). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2274059
- author
- Pavon Orozco, Patricia LU ; Santiago-Hernández, Alejandro ; Rosengren, Anna LU ; Hidalgo-Lara, María Eugenia and Stålbrand, Henrik LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Bioresource Technology
- volume
- 104
- pages
- 622 - 630
- publisher
- Elsevier
- external identifiers
-
- wos:000301155800084
- pmid:22169213
- scopus:84655163970
- pmid:22169213
- ISSN
- 1873-2976
- DOI
- 10.1016/j.biortech.2011.11.068
- language
- English
- LU publication?
- yes
- id
- 798c9305-b469-409e-a9cc-6966c5bb0912 (old id 2274059)
- date added to LUP
- 2016-04-01 13:04:46
- date last changed
- 2022-04-21 19:34:29
@article{798c9305-b469-409e-a9cc-6966c5bb0912, abstract = {{Synergy between Cellulomonas flavigena xylanase CflXyn11A and Trichoderma reesei endoglucanase TrCel7B was assessed during hydrolysis of alkaline pretreated sugar cane bagasse (SCB) after 12-48h, applying the individual enzymes and mixtures of the enzymes. A high degree of synergy (6.3) between CflXyn11A and TrCel7B in hydrolysis of SCB was observed after 12h in the equimolar mixture. A threefold decrease in the degree of synergy was observed with TrCel7B and the catalytic module of CflXyn11A; suggesting an important role played by the carbohydrate-binding module of CflXyn11A (CflXyn11A-CBM) in the observed synergy. Affinity electrophoresis and binding assays showed that CflXyn11A-CBM binds to xylans and to a lesser extent to cellulose. Our results suggest that synergy is more pronounced at early stages of hydrolysis. Furthermore, for the first time it is described that a CBM carried by a xylanase significantly enhances the synergy with a cellulase (threefold increase in synergy).}}, author = {{Pavon Orozco, Patricia and Santiago-Hernández, Alejandro and Rosengren, Anna and Hidalgo-Lara, María Eugenia and Stålbrand, Henrik}}, issn = {{1873-2976}}, language = {{eng}}, pages = {{622--630}}, publisher = {{Elsevier}}, series = {{Bioresource Technology}}, title = {{The family II carbohydrate-binding module of xylanase CflXyn11A from Cellulomonas flavigena increases the synergy with cellulase TrCel7B from Trichoderma reesei during the hydrolysis of sugar cane bagasse.}}, url = {{http://dx.doi.org/10.1016/j.biortech.2011.11.068}}, doi = {{10.1016/j.biortech.2011.11.068}}, volume = {{104}}, year = {{2012}}, }