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The family II carbohydrate-binding module of xylanase CflXyn11A from Cellulomonas flavigena increases the synergy with cellulase TrCel7B from Trichoderma reesei during the hydrolysis of sugar cane bagasse.

Pavon Orozco, Patricia LU ; Santiago-Hernández, Alejandro ; Rosengren, Anna LU ; Hidalgo-Lara, María Eugenia and Stålbrand, Henrik LU (2012) In Bioresource Technology 104. p.622-630
Abstract
Synergy between Cellulomonas flavigena xylanase CflXyn11A and Trichoderma reesei endoglucanase TrCel7B was assessed during hydrolysis of alkaline pretreated sugar cane bagasse (SCB) after 12-48h, applying the individual enzymes and mixtures of the enzymes. A high degree of synergy (6.3) between CflXyn11A and TrCel7B in hydrolysis of SCB was observed after 12h in the equimolar mixture. A threefold decrease in the degree of synergy was observed with TrCel7B and the catalytic module of CflXyn11A; suggesting an important role played by the carbohydrate-binding module of CflXyn11A (CflXyn11A-CBM) in the observed synergy. Affinity electrophoresis and binding assays showed that CflXyn11A-CBM binds to xylans and to a lesser extent to cellulose.... (More)
Synergy between Cellulomonas flavigena xylanase CflXyn11A and Trichoderma reesei endoglucanase TrCel7B was assessed during hydrolysis of alkaline pretreated sugar cane bagasse (SCB) after 12-48h, applying the individual enzymes and mixtures of the enzymes. A high degree of synergy (6.3) between CflXyn11A and TrCel7B in hydrolysis of SCB was observed after 12h in the equimolar mixture. A threefold decrease in the degree of synergy was observed with TrCel7B and the catalytic module of CflXyn11A; suggesting an important role played by the carbohydrate-binding module of CflXyn11A (CflXyn11A-CBM) in the observed synergy. Affinity electrophoresis and binding assays showed that CflXyn11A-CBM binds to xylans and to a lesser extent to cellulose. Our results suggest that synergy is more pronounced at early stages of hydrolysis. Furthermore, for the first time it is described that a CBM carried by a xylanase significantly enhances the synergy with a cellulase (threefold increase in synergy). (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Bioresource Technology
volume
104
pages
622 - 630
publisher
Elsevier
external identifiers
  • wos:000301155800084
  • pmid:22169213
  • scopus:84655163970
  • pmid:22169213
ISSN
1873-2976
DOI
10.1016/j.biortech.2011.11.068
language
English
LU publication?
yes
id
798c9305-b469-409e-a9cc-6966c5bb0912 (old id 2274059)
date added to LUP
2016-04-01 13:04:46
date last changed
2022-04-21 19:34:29
@article{798c9305-b469-409e-a9cc-6966c5bb0912,
  abstract     = {{Synergy between Cellulomonas flavigena xylanase CflXyn11A and Trichoderma reesei endoglucanase TrCel7B was assessed during hydrolysis of alkaline pretreated sugar cane bagasse (SCB) after 12-48h, applying the individual enzymes and mixtures of the enzymes. A high degree of synergy (6.3) between CflXyn11A and TrCel7B in hydrolysis of SCB was observed after 12h in the equimolar mixture. A threefold decrease in the degree of synergy was observed with TrCel7B and the catalytic module of CflXyn11A; suggesting an important role played by the carbohydrate-binding module of CflXyn11A (CflXyn11A-CBM) in the observed synergy. Affinity electrophoresis and binding assays showed that CflXyn11A-CBM binds to xylans and to a lesser extent to cellulose. Our results suggest that synergy is more pronounced at early stages of hydrolysis. Furthermore, for the first time it is described that a CBM carried by a xylanase significantly enhances the synergy with a cellulase (threefold increase in synergy).}},
  author       = {{Pavon Orozco, Patricia and Santiago-Hernández, Alejandro and Rosengren, Anna and Hidalgo-Lara, María Eugenia and Stålbrand, Henrik}},
  issn         = {{1873-2976}},
  language     = {{eng}},
  pages        = {{622--630}},
  publisher    = {{Elsevier}},
  series       = {{Bioresource Technology}},
  title        = {{The family II carbohydrate-binding module of xylanase CflXyn11A from Cellulomonas flavigena increases the synergy with cellulase TrCel7B from Trichoderma reesei during the hydrolysis of sugar cane bagasse.}},
  url          = {{http://dx.doi.org/10.1016/j.biortech.2011.11.068}},
  doi          = {{10.1016/j.biortech.2011.11.068}},
  volume       = {{104}},
  year         = {{2012}},
}