Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Sol-gel integrated protein microarray for high-resolution signal readout of psa (prostate specific antigen) in clinical samples

Lee, S. W. LU ; Ahn, J. Y. ; Järås, K. LU ; Lilja, H. LU orcid ; Jo, M. J. ; Jung, C. Y. ; Jeong, O. C. ; Kim, S. Y. and Laurell, T. LU (2010) 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 2. p.800-802
Abstract

In this study, we demonstrate a new protein microarray technology for highly sensitive detection of PSA (prostate caser specific antigen) in scrum samples. Using the optically active sol-gel nanocomposites, which can hold capturing probes in native and surface morphology tailored porous silicon, purified PSA (Prostate Specific Antigen) in human female serum was assessed by FlTC-antiPSA in broad dynamic range of the sandwich (1 pg/mL to Ing/mL). Additionally, we tested reverse phase assay using our developed system, which purified PSA imbedded in sol-gel and FITC labeled its counter antibody was accessed. Dynamic range was 60 fg/mL to 6ng/mL. Our concept can allow the measurement oflow amount of PSA at pg/ml range and thus, it is... (More)

In this study, we demonstrate a new protein microarray technology for highly sensitive detection of PSA (prostate caser specific antigen) in scrum samples. Using the optically active sol-gel nanocomposites, which can hold capturing probes in native and surface morphology tailored porous silicon, purified PSA (Prostate Specific Antigen) in human female serum was assessed by FlTC-antiPSA in broad dynamic range of the sandwich (1 pg/mL to Ing/mL). Additionally, we tested reverse phase assay using our developed system, which purified PSA imbedded in sol-gel and FITC labeled its counter antibody was accessed. Dynamic range was 60 fg/mL to 6ng/mL. Our concept can allow the measurement oflow amount of PSA at pg/ml range and thus, it is possible to do relative quantification for marker protein as well.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Porous silicon, Sol-gel microarray. PSA
host publication
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
series title
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
volume
2
pages
800 - 802
conference name
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
conference location
Groningen, Netherlands
conference dates
2010-10-03 - 2010-10-07
external identifiers
  • scopus:84884406206
ISBN
9781618390622
language
English
LU publication?
yes
id
7a4ce10f-4a8f-4836-9e44-6c9018257e94
date added to LUP
2022-12-08 12:22:00
date last changed
2022-12-09 09:29:21
@inproceedings{7a4ce10f-4a8f-4836-9e44-6c9018257e94,
  abstract     = {{<p>In this study, we demonstrate a new protein microarray technology for highly sensitive detection of PSA (prostate caser specific antigen) in scrum samples. Using the optically active sol-gel nanocomposites, which can hold capturing probes in native and surface morphology tailored porous silicon, purified PSA (Prostate Specific Antigen) in human female serum was assessed by FlTC-antiPSA in broad dynamic range of the sandwich (1 pg/mL to Ing/mL). Additionally, we tested reverse phase assay using our developed system, which purified PSA imbedded in sol-gel and FITC labeled its counter antibody was accessed. Dynamic range was 60 fg/mL to 6ng/mL. Our concept can allow the measurement oflow amount of PSA at pg/ml range and thus, it is possible to do relative quantification for marker protein as well.</p>}},
  author       = {{Lee, S. W. and Ahn, J. Y. and Järås, K. and Lilja, H. and Jo, M. J. and Jung, C. Y. and Jeong, O. C. and Kim, S. Y. and Laurell, T.}},
  booktitle    = {{14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010}},
  isbn         = {{9781618390622}},
  keywords     = {{Porous silicon; Sol-gel microarray. PSA}},
  language     = {{eng}},
  pages        = {{800--802}},
  series       = {{14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010}},
  title        = {{Sol-gel integrated protein microarray for high-resolution signal readout of psa (prostate specific antigen) in clinical samples}},
  volume       = {{2}},
  year         = {{2010}},
}