Sol-gel integrated protein microarray for high-resolution signal readout of psa (prostate specific antigen) in clinical samples
(2010) 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 2. p.800-802- Abstract
In this study, we demonstrate a new protein microarray technology for highly sensitive detection of PSA (prostate caser specific antigen) in scrum samples. Using the optically active sol-gel nanocomposites, which can hold capturing probes in native and surface morphology tailored porous silicon, purified PSA (Prostate Specific Antigen) in human female serum was assessed by FlTC-antiPSA in broad dynamic range of the sandwich (1 pg/mL to Ing/mL). Additionally, we tested reverse phase assay using our developed system, which purified PSA imbedded in sol-gel and FITC labeled its counter antibody was accessed. Dynamic range was 60 fg/mL to 6ng/mL. Our concept can allow the measurement oflow amount of PSA at pg/ml range and thus, it is... (More)
In this study, we demonstrate a new protein microarray technology for highly sensitive detection of PSA (prostate caser specific antigen) in scrum samples. Using the optically active sol-gel nanocomposites, which can hold capturing probes in native and surface morphology tailored porous silicon, purified PSA (Prostate Specific Antigen) in human female serum was assessed by FlTC-antiPSA in broad dynamic range of the sandwich (1 pg/mL to Ing/mL). Additionally, we tested reverse phase assay using our developed system, which purified PSA imbedded in sol-gel and FITC labeled its counter antibody was accessed. Dynamic range was 60 fg/mL to 6ng/mL. Our concept can allow the measurement oflow amount of PSA at pg/ml range and thus, it is possible to do relative quantification for marker protein as well.
(Less)
- author
- Lee, S. W.
LU
; Ahn, J. Y.
; Järås, K.
LU
; Lilja, H.
LU
; Jo, M. J. ; Jung, C. Y. ; Jeong, O. C. ; Kim, S. Y. and Laurell, T. LU
- organization
- publishing date
- 2010
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Porous silicon, Sol-gel microarray. PSA
- host publication
- 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
- series title
- 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
- volume
- 2
- pages
- 800 - 802
- conference name
- 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
- conference location
- Groningen, Netherlands
- conference dates
- 2010-10-03 - 2010-10-07
- external identifiers
-
- scopus:84884406206
- ISBN
- 9781618390622
- language
- English
- LU publication?
- yes
- id
- 7a4ce10f-4a8f-4836-9e44-6c9018257e94
- date added to LUP
- 2022-12-08 12:22:00
- date last changed
- 2025-01-10 15:14:57
@inproceedings{7a4ce10f-4a8f-4836-9e44-6c9018257e94, abstract = {{<p>In this study, we demonstrate a new protein microarray technology for highly sensitive detection of PSA (prostate caser specific antigen) in scrum samples. Using the optically active sol-gel nanocomposites, which can hold capturing probes in native and surface morphology tailored porous silicon, purified PSA (Prostate Specific Antigen) in human female serum was assessed by FlTC-antiPSA in broad dynamic range of the sandwich (1 pg/mL to Ing/mL). Additionally, we tested reverse phase assay using our developed system, which purified PSA imbedded in sol-gel and FITC labeled its counter antibody was accessed. Dynamic range was 60 fg/mL to 6ng/mL. Our concept can allow the measurement oflow amount of PSA at pg/ml range and thus, it is possible to do relative quantification for marker protein as well.</p>}}, author = {{Lee, S. W. and Ahn, J. Y. and Järås, K. and Lilja, H. and Jo, M. J. and Jung, C. Y. and Jeong, O. C. and Kim, S. Y. and Laurell, T.}}, booktitle = {{14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010}}, isbn = {{9781618390622}}, keywords = {{Porous silicon; Sol-gel microarray. PSA}}, language = {{eng}}, pages = {{800--802}}, series = {{14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010}}, title = {{Sol-gel integrated protein microarray for high-resolution signal readout of psa (prostate specific antigen) in clinical samples}}, volume = {{2}}, year = {{2010}}, }