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Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase

Walfridsson, M; Bao, X; Anderlund, M LU ; Lilius, G LU ; Bülow, L LU and Hahn-Hägerdal, B LU (1996) In Applied and Environmental Microbiology 62(12). p.51-4648
Abstract

The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. The recombinant xylose isomerase showed the highest activity at 85 degrees C with a specific activity of 1.0 U mg-1. A new functional metabolic pathway in S. cerevisiae with ethanol formation during oxygen-limited xylose fermentation was demonstrated. Xylitol and acetic acid were also formed during the fermentation.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aldose-Ketose Isomerases, Base Sequence, Carbohydrate Epimerases, Ethanol, Fermentation, Genes, Bacterial, Molecular Sequence Data, Phosphoglycerate Kinase, Recombinant Proteins, Saccharomyces cerevisiae, Thermus thermophilus, Xylose
in
Applied and Environmental Microbiology
volume
62
issue
12
pages
4 pages
publisher
American Society for Microbiology
external identifiers
  • scopus:0029909726
ISSN
0099-2240
language
English
LU publication?
yes
id
7abdaa5b-de47-42d6-b033-1a5e1f263eb0
alternative location
http://aem.asm.org/content/62/12/4648.abstract
date added to LUP
2016-04-18 16:01:19
date last changed
2017-05-21 04:50:02
@article{7abdaa5b-de47-42d6-b033-1a5e1f263eb0,
  abstract     = {<p>The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. The recombinant xylose isomerase showed the highest activity at 85 degrees C with a specific activity of 1.0 U mg-1. A new functional metabolic pathway in S. cerevisiae with ethanol formation during oxygen-limited xylose fermentation was demonstrated. Xylitol and acetic acid were also formed during the fermentation.</p>},
  author       = {Walfridsson, M and Bao, X and Anderlund, M and Lilius, G and Bülow, L and Hahn-Hägerdal, B},
  issn         = {0099-2240},
  keyword      = {Aldose-Ketose Isomerases,Base Sequence,Carbohydrate Epimerases,Ethanol,Fermentation,Genes, Bacterial,Molecular Sequence Data,Phosphoglycerate Kinase,Recombinant Proteins,Saccharomyces cerevisiae,Thermus thermophilus,Xylose},
  language     = {eng},
  number       = {12},
  pages        = {51--4648},
  publisher    = {American Society for Microbiology},
  series       = {Applied and Environmental Microbiology},
  title        = {Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase},
  volume       = {62},
  year         = {1996},
}