Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase
(1996) In Applied and Environmental Microbiology 62(12). p.51-4648- Abstract
The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. The recombinant xylose isomerase showed the highest activity at 85 degrees C with a specific activity of 1.0 U mg-1. A new functional metabolic pathway in S. cerevisiae with ethanol formation during oxygen-limited xylose fermentation was demonstrated. Xylitol and acetic acid were also formed during the fermentation.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7abdaa5b-de47-42d6-b033-1a5e1f263eb0
- author
- Walfridsson, M ; Bao, X ; Anderlund, M LU ; Lilius, G LU ; Bülow, L LU and Hahn-Hägerdal, B LU
- organization
- publishing date
- 1996-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Aldose-Ketose Isomerases, Base Sequence, Carbohydrate Epimerases, Ethanol, Fermentation, Genes, Bacterial, Molecular Sequence Data, Phosphoglycerate Kinase, Recombinant Proteins, Saccharomyces cerevisiae, Thermus thermophilus, Xylose
- in
- Applied and Environmental Microbiology
- volume
- 62
- issue
- 12
- pages
- 4 pages
- publisher
- American Society for Microbiology
- external identifiers
-
- pmid:8953736
- scopus:0029909726
- ISSN
- 0099-2240
- language
- English
- LU publication?
- yes
- id
- 7abdaa5b-de47-42d6-b033-1a5e1f263eb0
- alternative location
- http://aem.asm.org/content/62/12/4648.abstract
- date added to LUP
- 2016-04-18 16:01:19
- date last changed
- 2024-06-15 04:14:00
@article{7abdaa5b-de47-42d6-b033-1a5e1f263eb0, abstract = {{<p>The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. The recombinant xylose isomerase showed the highest activity at 85 degrees C with a specific activity of 1.0 U mg-1. A new functional metabolic pathway in S. cerevisiae with ethanol formation during oxygen-limited xylose fermentation was demonstrated. Xylitol and acetic acid were also formed during the fermentation.</p>}}, author = {{Walfridsson, M and Bao, X and Anderlund, M and Lilius, G and Bülow, L and Hahn-Hägerdal, B}}, issn = {{0099-2240}}, keywords = {{Aldose-Ketose Isomerases; Base Sequence; Carbohydrate Epimerases; Ethanol; Fermentation; Genes, Bacterial; Molecular Sequence Data; Phosphoglycerate Kinase; Recombinant Proteins; Saccharomyces cerevisiae; Thermus thermophilus; Xylose}}, language = {{eng}}, number = {{12}}, pages = {{51--4648}}, publisher = {{American Society for Microbiology}}, series = {{Applied and Environmental Microbiology}}, title = {{Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase}}, url = {{http://aem.asm.org/content/62/12/4648.abstract}}, volume = {{62}}, year = {{1996}}, }