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Separation of pathogenic bacteria by chain length

Beech, Jason P. LU ; Ho, Bao Dang LU ; Garriss, Geneviève; Oliveira, Vitor; Henriques-Normark, Birgitta and Tegenfeldt, Jonas O. LU (2018) In Analytica Chimica Acta 1000. p.223-231
Abstract

Using Deterministic Lateral Displacement devices optimized for sensitivity to particle length, we separate subpopulations of bacteria depending on known properties that affect their capability to cause disease (virulence). For the human bacterial pathogen Streptococcus pneumoniae, bacterial chain length and the presence of a capsule are known virulence factors contributing to its ability to cause severe disease. Separation of cultured pneumococci into subpopulations based on morphological type (single cocci, diplococci and chains) will enable more detailed studies of the role they play in virulence. Moreover, we present separation of mixed populations of almost genetically identical encapsulated and non-encapsulated pneumococcal strains... (More)

Using Deterministic Lateral Displacement devices optimized for sensitivity to particle length, we separate subpopulations of bacteria depending on known properties that affect their capability to cause disease (virulence). For the human bacterial pathogen Streptococcus pneumoniae, bacterial chain length and the presence of a capsule are known virulence factors contributing to its ability to cause severe disease. Separation of cultured pneumococci into subpopulations based on morphological type (single cocci, diplococci and chains) will enable more detailed studies of the role they play in virulence. Moreover, we present separation of mixed populations of almost genetically identical encapsulated and non-encapsulated pneumococcal strains in our device.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bacterial virulence, Deterministic lateral displacement, Morphology, Separation
in
Analytica Chimica Acta
volume
1000
pages
223 - 231
publisher
Elsevier
external identifiers
  • scopus:85036624788
ISSN
0003-2670
DOI
language
English
LU publication?
yes
id
7b6dc176-45fc-4b79-87de-41103dc3ce7d
date added to LUP
2018-01-02 12:46:45
date last changed
2018-05-29 11:32:59
@article{7b6dc176-45fc-4b79-87de-41103dc3ce7d,
  abstract     = {<p>Using Deterministic Lateral Displacement devices optimized for sensitivity to particle length, we separate subpopulations of bacteria depending on known properties that affect their capability to cause disease (virulence). For the human bacterial pathogen Streptococcus pneumoniae, bacterial chain length and the presence of a capsule are known virulence factors contributing to its ability to cause severe disease. Separation of cultured pneumococci into subpopulations based on morphological type (single cocci, diplococci and chains) will enable more detailed studies of the role they play in virulence. Moreover, we present separation of mixed populations of almost genetically identical encapsulated and non-encapsulated pneumococcal strains in our device.</p>},
  author       = {Beech, Jason P. and Ho, Bao Dang and Garriss, Geneviève and Oliveira, Vitor and Henriques-Normark, Birgitta and Tegenfeldt, Jonas O.},
  issn         = {0003-2670},
  keyword      = {Bacterial virulence,Deterministic lateral displacement,Morphology,Separation},
  language     = {eng},
  pages        = {223--231},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {Separation of pathogenic bacteria by chain length},
  url          = {http://dx.doi.org/},
  volume       = {1000},
  year         = {2018},
}