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Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates

Shikhagaie, Medya Mara LU ; Björklund, Åsa K.; Mjösberg, Jenny; Erjefält, Jonas S. LU ; Cornelissen, Anne S.; Ros, Xavier Romero; Bal, Suzanne M.; Koning, Jasper J.; Mebius, Reina E. and Mori, Michiko LU , et al. (2017) In Cell Reports 18(7). p.1761-1773
Abstract

Here, we characterize a subset of ILC3s that express Neuropilin1 (NRP1) and are present in lymphoid tissues, but not in the peripheral blood or skin. NRP1+ group 3 innate lymphoid cells (ILC3s) display in vitro lymphoid tissue inducer (LTi) activity. In agreement with this, NRP1+ ILC3s are mainly located in proximity to high endothelial venules (HEVs) and express cell surface molecules involved in lymphocyte migration in secondary lymphoid tissues via HEVs. NRP1 was also expressed on mouse fetal LTi cells, indicating that NRP1 is a conserved marker for LTi cells. Human NRP1+ ILC3s are primed cells because they express CD45RO and produce higher amounts of cytokines than NRP1 cells, which... (More)

Here, we characterize a subset of ILC3s that express Neuropilin1 (NRP1) and are present in lymphoid tissues, but not in the peripheral blood or skin. NRP1+ group 3 innate lymphoid cells (ILC3s) display in vitro lymphoid tissue inducer (LTi) activity. In agreement with this, NRP1+ ILC3s are mainly located in proximity to high endothelial venules (HEVs) and express cell surface molecules involved in lymphocyte migration in secondary lymphoid tissues via HEVs. NRP1 was also expressed on mouse fetal LTi cells, indicating that NRP1 is a conserved marker for LTi cells. Human NRP1+ ILC3s are primed cells because they express CD45RO and produce higher amounts of cytokines than NRP1 cells, which express CD45RA. The NRP1 ligand vascular endothelial growth factor A (VEGF-A) served as a chemotactic factor for NRP1+ ILC3s. NRP1+ ILC3s are present in lung tissues from smokers and patients with chronic obstructive pulmonary disease, suggesting a role in angiogenesis and/or the initiation of ectopic pulmonary lymphoid aggregates.

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keywords
chemotaxis, HEVs, high endothelial venues, human innate lymphoid cells 3, ILC3, LA, LTi, lymphoid tissue inducer cells, neuropilin-1, NRP1, smoke-induced pulmonary lymphoid aggregates, vascular endothelial growth factor A, VEGF-A
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Cell Reports
volume
18
issue
7
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13 pages
publisher
Cell Press
external identifiers
  • scopus:85013188332
  • wos:000397324900017
ISSN
2211-1247
DOI
10.1016/j.celrep.2017.01.063
language
English
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yes
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7b98bfe3-21b8-4c98-9524-cfa663c12923
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2017-03-01 10:34:18
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2018-04-29 04:36:45
@article{7b98bfe3-21b8-4c98-9524-cfa663c12923,
  abstract     = {<p>Here, we characterize a subset of ILC3s that express Neuropilin1 (NRP1) and are present in lymphoid tissues, but not in the peripheral blood or skin. NRP1<sup>+</sup> group 3 innate lymphoid cells (ILC3s) display in vitro lymphoid tissue inducer (LTi) activity. In agreement with this, NRP1<sup>+</sup> ILC3s are mainly located in proximity to high endothelial venules (HEVs) and express cell surface molecules involved in lymphocyte migration in secondary lymphoid tissues via HEVs. NRP1 was also expressed on mouse fetal LTi cells, indicating that NRP1 is a conserved marker for LTi cells. Human NRP1<sup>+</sup> ILC3s are primed cells because they express CD45RO and produce higher amounts of cytokines than NRP1<sup>−</sup> cells, which express CD45RA. The NRP1 ligand vascular endothelial growth factor A (VEGF-A) served as a chemotactic factor for NRP1<sup>+</sup> ILC3s. NRP1<sup>+</sup> ILC3s are present in lung tissues from smokers and patients with chronic obstructive pulmonary disease, suggesting a role in angiogenesis and/or the initiation of ectopic pulmonary lymphoid aggregates.</p>},
  author       = {Shikhagaie, Medya Mara and Björklund, Åsa K. and Mjösberg, Jenny and Erjefält, Jonas S. and Cornelissen, Anne S. and Ros, Xavier Romero and Bal, Suzanne M. and Koning, Jasper J. and Mebius, Reina E. and Mori, Michiko and Bruchard, Melanie and Blom, Bianca and Spits, Hergen},
  issn         = {2211-1247},
  keyword      = {chemotaxis,HEVs,high endothelial venues,human innate lymphoid cells 3,ILC3,LA,LTi,lymphoid tissue inducer cells,neuropilin-1,NRP1,smoke-induced pulmonary lymphoid aggregates,vascular endothelial growth factor A,VEGF-A},
  language     = {eng},
  month        = {02},
  number       = {7},
  pages        = {1761--1773},
  publisher    = {Cell Press},
  series       = {Cell Reports},
  title        = {Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates},
  url          = {http://dx.doi.org/10.1016/j.celrep.2017.01.063},
  volume       = {18},
  year         = {2017},
}