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Superresolution imaging of the cytoplasmic phosphatase PTPN22 links integrin-mediated T cell adhesion with autoimmunity

Burn, Garth L.; Cornish, Georgina H.; Potrzebowska, Kasia LU ; Samuelsson, Malin LU ; Griffié, Juliette; Minoughan, Sophie; Yates, Mark; Ashdown, George; Pernodet, Nicolas and Morrison, Vicky L., et al. (2016) In Science Signaling 9(448).
Abstract

Integrins are heterodimeric transmembrane proteins that play a fundamental role in the migration of leukocytes to sites of infection or injury. We found that protein tyrosine phosphatase nonreceptor type 22 (PTPN22) inhibits signaling by the integrin lymphocyte function-associated antigen-1 (LFA-1) in effector T cells. PTPN22 colocalized with its substrates at the leading edge of cells migrating on surfaces coated with the LFA-1 ligand intercellular adhesion molecule-1 (ICAM-1). Knockout or knockdown of PTPN22 or expression of the autoimmune disease-associated PTPN22-R620W variant resulted in the enhanced phosphorylation of signaling molecules downstream of integrins. Superresolution imaging revealed that PTPN22-R620 (wild-type PTPN22)... (More)

Integrins are heterodimeric transmembrane proteins that play a fundamental role in the migration of leukocytes to sites of infection or injury. We found that protein tyrosine phosphatase nonreceptor type 22 (PTPN22) inhibits signaling by the integrin lymphocyte function-associated antigen-1 (LFA-1) in effector T cells. PTPN22 colocalized with its substrates at the leading edge of cells migrating on surfaces coated with the LFA-1 ligand intercellular adhesion molecule-1 (ICAM-1). Knockout or knockdown of PTPN22 or expression of the autoimmune disease-associated PTPN22-R620W variant resulted in the enhanced phosphorylation of signaling molecules downstream of integrins. Superresolution imaging revealed that PTPN22-R620 (wild-type PTPN22) was present as large clusters in unstimulated T cells and that these disaggregated upon stimulation of LFA-1, enabling increased association of PTPN22 with its binding partners at the leading edge. The failure of PTPN22-R620W molecules to be retained at the leading edge led to increased LFA-1 clustering and integrin-mediated cell adhesion. Our data define a previously uncharacterized mechanism for fine-tuning integrin signaling in T cells, as well as a paradigm of auto-immunity in humans in which disease susceptibility is underpinned by inherited phosphatase mutations that perturb integrin function. 2016

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Science Signaling
volume
9
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448
publisher
American Association for the Advancement of Science (AAAS)
external identifiers
  • scopus:84990215625
  • wos:000387027200004
ISSN
1945-0877
DOI
10.1126/scisignal.aaf2195
language
English
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yes
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7c2a11d5-c165-4421-8746-af71be0fff8f
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2016-10-21 08:42:04
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2017-10-29 04:53:03
@article{7c2a11d5-c165-4421-8746-af71be0fff8f,
  abstract     = {<p>Integrins are heterodimeric transmembrane proteins that play a fundamental role in the migration of leukocytes to sites of infection or injury. We found that protein tyrosine phosphatase nonreceptor type 22 (PTPN22) inhibits signaling by the integrin lymphocyte function-associated antigen-1 (LFA-1) in effector T cells. PTPN22 colocalized with its substrates at the leading edge of cells migrating on surfaces coated with the LFA-1 ligand intercellular adhesion molecule-1 (ICAM-1). Knockout or knockdown of PTPN22 or expression of the autoimmune disease-associated PTPN22-R620W variant resulted in the enhanced phosphorylation of signaling molecules downstream of integrins. Superresolution imaging revealed that PTPN22-R620 (wild-type PTPN22) was present as large clusters in unstimulated T cells and that these disaggregated upon stimulation of LFA-1, enabling increased association of PTPN22 with its binding partners at the leading edge. The failure of PTPN22-R620W molecules to be retained at the leading edge led to increased LFA-1 clustering and integrin-mediated cell adhesion. Our data define a previously uncharacterized mechanism for fine-tuning integrin signaling in T cells, as well as a paradigm of auto-immunity in humans in which disease susceptibility is underpinned by inherited phosphatase mutations that perturb integrin function. 2016</p>},
  articleno    = {ra99},
  author       = {Burn, Garth L. and Cornish, Georgina H. and Potrzebowska, Kasia and Samuelsson, Malin and Griffié, Juliette and Minoughan, Sophie and Yates, Mark and Ashdown, George and Pernodet, Nicolas and Morrison, Vicky L. and Sanchez-Blanco, Cristina and Purvis, Harriet and Clarke, Fiona and Brownlie, Rebecca J. and Vyse, Timothy J. and Zamoyska, Rose and Owen, Dylan M. and Svensson, Lena M. and Cope, Andrew P.},
  issn         = {1945-0877},
  language     = {eng},
  month        = {10},
  number       = {448},
  publisher    = {American Association for the Advancement of Science (AAAS)},
  series       = {Science Signaling},
  title        = {Superresolution imaging of the cytoplasmic phosphatase PTPN22 links integrin-mediated T cell adhesion with autoimmunity},
  url          = {http://dx.doi.org/10.1126/scisignal.aaf2195},
  volume       = {9},
  year         = {2016},
}