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Axonal outgrowth from adult mouse nodose ganglia In vitro is stimulated by neurotrophin-4 in a Trk receptor and mitogen-activated protein kinase-dependent way

Wiklund, Peter LU and Ekström, Per LU (2000) In Journal of Neurobiology 45(3). p.142-151
Abstract

The actions of neurotrophic factors on sensory neurons of the adult nodose ganglion were studied in vitro. The ganglia were explanted in an extracellular matrix-based gel that permitted observation of the growing axons. Neurotrophin-4 (NT-4) was a very efficient stimulator of outgrowth of axons from the nodose ganglion and had almost doubled the outgrowth length when this was analyzed after 2 days in culture. Brain-derived neurotrophic factor also stimulated outgrowth, but to a lesser degree, whereas NT-3 gave only weak stimulatory tendencies. Nerve growth factor and glial cell line-derived neurotrophic factor both lacked stimulatory effects. NT-4 is known to act via TrkB receptors, and the presence of these on growing nodose neurons... (More)

The actions of neurotrophic factors on sensory neurons of the adult nodose ganglion were studied in vitro. The ganglia were explanted in an extracellular matrix-based gel that permitted observation of the growing axons. Neurotrophin-4 (NT-4) was a very efficient stimulator of outgrowth of axons from the nodose ganglion and had almost doubled the outgrowth length when this was analyzed after 2 days in culture. Brain-derived neurotrophic factor also stimulated outgrowth, but to a lesser degree, whereas NT-3 gave only weak stimulatory tendencies. Nerve growth factor and glial cell line-derived neurotrophic factor both lacked stimulatory effects. NT-4 is known to act via TrkB receptors, and the presence of these on growing nodose neurons was demonstrated immunohistochemically. In line with a Trk-mediated growth effect, the NT-4 stimulation was abolished by K252a, a selective inhibitor of neurotrophin receptor-associated tyrosine kinase activity. K252a had no effect on the unstimulated preparation. NT-4 treatment led to activation of the mitogen-activated protein kinase and inhibition of the latter pathway by PD98059 significantly reduced the NT-4 stimulated outgrowth, whereas the drug had no effect on the unstimulated growth. In conclusion, the data suggest that NT-4 can serve as a powerful growth factor for neurons of adult nodose ganglia and that the growth stimulation involves TrkB- and mitogen-activated protein kinase. (C) 2000 John Wiley and Sons, Inc.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
BDNF, Brain-derived neurotrophic factor, Regeneration, Sensory neurons, Vagus
in
Journal of Neurobiology
volume
45
issue
3
pages
10 pages
publisher
John Wiley and Sons Inc.
external identifiers
  • scopus:0034669687
ISSN
0022-3034
DOI
10.1002/1097-4695(20001115)45:3<142::AID-NEU2>3.0.CO;2-4
language
English
LU publication?
yes
id
7c534b70-69be-4036-a473-f86cc12acf3c
date added to LUP
2016-12-07 14:48:10
date last changed
2017-01-26 17:17:30
@article{7c534b70-69be-4036-a473-f86cc12acf3c,
  abstract     = {<p>The actions of neurotrophic factors on sensory neurons of the adult nodose ganglion were studied in vitro. The ganglia were explanted in an extracellular matrix-based gel that permitted observation of the growing axons. Neurotrophin-4 (NT-4) was a very efficient stimulator of outgrowth of axons from the nodose ganglion and had almost doubled the outgrowth length when this was analyzed after 2 days in culture. Brain-derived neurotrophic factor also stimulated outgrowth, but to a lesser degree, whereas NT-3 gave only weak stimulatory tendencies. Nerve growth factor and glial cell line-derived neurotrophic factor both lacked stimulatory effects. NT-4 is known to act via TrkB receptors, and the presence of these on growing nodose neurons was demonstrated immunohistochemically. In line with a Trk-mediated growth effect, the NT-4 stimulation was abolished by K252a, a selective inhibitor of neurotrophin receptor-associated tyrosine kinase activity. K252a had no effect on the unstimulated preparation. NT-4 treatment led to activation of the mitogen-activated protein kinase and inhibition of the latter pathway by PD98059 significantly reduced the NT-4 stimulated outgrowth, whereas the drug had no effect on the unstimulated growth. In conclusion, the data suggest that NT-4 can serve as a powerful growth factor for neurons of adult nodose ganglia and that the growth stimulation involves TrkB- and mitogen-activated protein kinase. (C) 2000 John Wiley and Sons, Inc.</p>},
  author       = {Wiklund, Peter and Ekström, Per},
  issn         = {0022-3034},
  keyword      = {BDNF,Brain-derived neurotrophic factor,Regeneration,Sensory neurons,Vagus},
  language     = {eng},
  month        = {11},
  number       = {3},
  pages        = {142--151},
  publisher    = {John Wiley and Sons Inc.},
  series       = {Journal of Neurobiology},
  title        = {Axonal outgrowth from adult mouse nodose ganglia In vitro is stimulated by neurotrophin-4 in a Trk receptor and mitogen-activated protein kinase-dependent way},
  url          = {http://dx.doi.org/10.1002/1097-4695(20001115)45:3<142::AID-NEU2>3.0.CO;2-4},
  volume       = {45},
  year         = {2000},
}