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Rapid tracing of resistance plasmids in a nosocomial outbreak using optical DNA mapping

Müller, Vilhelm; Karami, Nahid; Nyberg, Lena K.; Pichler, Christoffer LU ; Torche Pedreschi, Paola C.; Quaderi, Saair; Fritzsche, Joachim; Ambjörnsson, Tobias LU ; Åhrén, Christina and Westerlund, Fredrik (2016) In ACS Infectious Diseases 2(5). p.322-328
Abstract

Resistance to life-saving antibiotics increases rapidly worldwide, and multiresistant bacteria have become a global threat to human health. Presently, the most serious threat is the increasing spread of Enterobacteriaceae carrying genes coding for extended spectrum β-lactamases (ESBL) and carbapenemases on highly mobile plasmids. We here demonstrate how optical DNA maps of single plasmids can be used as fingerprints to trace plasmids, for example, during resistance outbreaks. We use the assay to demonstrate a potential transmission route of an ESBL-carrying plasmid between bacterial strains/species and between patients, during a polyclonal outbreak at a neonatal ward at Sahlgrenska University Hospital (Gothenburg, Sweden). Our results... (More)

Resistance to life-saving antibiotics increases rapidly worldwide, and multiresistant bacteria have become a global threat to human health. Presently, the most serious threat is the increasing spread of Enterobacteriaceae carrying genes coding for extended spectrum β-lactamases (ESBL) and carbapenemases on highly mobile plasmids. We here demonstrate how optical DNA maps of single plasmids can be used as fingerprints to trace plasmids, for example, during resistance outbreaks. We use the assay to demonstrate a potential transmission route of an ESBL-carrying plasmid between bacterial strains/species and between patients, during a polyclonal outbreak at a neonatal ward at Sahlgrenska University Hospital (Gothenburg, Sweden). Our results demonstrate that optical DNA mapping is an easy and rapid method for detecting the spread of plasmids mediating resistance. With the increasing prevalence of multiresistant bacteria, diagnostic tools that can aid in solving ongoing routes of transmission, in particular in hospital settings, will be of paramount importance.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
antibiotic resistance, nosocomial outbreak, optical DNA mapping, plasmids
in
ACS Infectious Diseases
volume
2
issue
5
pages
7 pages
publisher
The American Chemical Society
external identifiers
  • scopus:84991518524
  • wos:000376145700003
DOI
10.1021/acsinfecdis.6b00017
language
English
LU publication?
yes
id
7e593245-7e4f-451a-b17c-9b334aed74d4
date added to LUP
2016-11-07 09:53:42
date last changed
2017-07-09 04:54:35
@article{7e593245-7e4f-451a-b17c-9b334aed74d4,
  abstract     = {<p>Resistance to life-saving antibiotics increases rapidly worldwide, and multiresistant bacteria have become a global threat to human health. Presently, the most serious threat is the increasing spread of Enterobacteriaceae carrying genes coding for extended spectrum β-lactamases (ESBL) and carbapenemases on highly mobile plasmids. We here demonstrate how optical DNA maps of single plasmids can be used as fingerprints to trace plasmids, for example, during resistance outbreaks. We use the assay to demonstrate a potential transmission route of an ESBL-carrying plasmid between bacterial strains/species and between patients, during a polyclonal outbreak at a neonatal ward at Sahlgrenska University Hospital (Gothenburg, Sweden). Our results demonstrate that optical DNA mapping is an easy and rapid method for detecting the spread of plasmids mediating resistance. With the increasing prevalence of multiresistant bacteria, diagnostic tools that can aid in solving ongoing routes of transmission, in particular in hospital settings, will be of paramount importance.</p>},
  author       = {Müller, Vilhelm and Karami, Nahid and Nyberg, Lena K. and Pichler, Christoffer and Torche Pedreschi, Paola C. and Quaderi, Saair and Fritzsche, Joachim and Ambjörnsson, Tobias and Åhrén, Christina and Westerlund, Fredrik},
  keyword      = {antibiotic resistance,nosocomial outbreak,optical DNA mapping,plasmids},
  language     = {eng},
  month        = {05},
  number       = {5},
  pages        = {322--328},
  publisher    = {The American Chemical Society},
  series       = {ACS Infectious Diseases},
  title        = {Rapid tracing of resistance plasmids in a nosocomial outbreak using optical DNA mapping},
  url          = {http://dx.doi.org/10.1021/acsinfecdis.6b00017},
  volume       = {2},
  year         = {2016},
}