A porous silicon immunoassay platform for fluorometric determination of alpha-synuclein in human cerebrospinal fluid
Lee, Sangwook ; Silajdzic, Edina LU ; Yang, Hon ; Björkqvist, Maria LU
; Kim, Soyon
; Jeong, Ok Chan
; Hansson, Oskar
LU
and Laurell, Thomas
LU
(2014)
In Microchimica Acta
181(9-10).
p.1143-1149
- Abstract
- Levels of total and/or oligomeric alpha-synuclein may be used as a biomarker tool to aid in the diagnosis and development of new disease-modifying therapies. We report here on a porous silicon antibody microarray for the fluorimetric determination of cerebrospinal fluid levels of total alpha-synuclein, a protein involved the pathology of Parkinson's disease. The surface of porous silicon has a 3-dimensional macro- and nanoporous structure, and this offers a large binding capacity for capturing probe molecules. Porous silicon also warrants efficient immobilization of antibodies by surface adsorption, and does not require chemical immobilization. The platform requires 10 mu L of cerebrospinal fluid, and each test requires 4 h for assay only... (More)
- Levels of total and/or oligomeric alpha-synuclein may be used as a biomarker tool to aid in the diagnosis and development of new disease-modifying therapies. We report here on a porous silicon antibody microarray for the fluorimetric determination of cerebrospinal fluid levels of total alpha-synuclein, a protein involved the pathology of Parkinson's disease. The surface of porous silicon has a 3-dimensional macro- and nanoporous structure, and this offers a large binding capacity for capturing probe molecules. Porous silicon also warrants efficient immobilization of antibodies by surface adsorption, and does not require chemical immobilization. The platform requires 10 mu L of cerebrospinal fluid, and each test requires 4 h for assay only (including immobilization of capturing antibody). The limit of detection is 35 pg mL(-1) of alpha-synuclein in cerebrospinal fluid, and the dynamic analytical range extends from 0.01 to 100 ng center dot mL(-1). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4602824
- author
- Lee, Sangwook
; Silajdzic, Edina
LU
; Yang, Hon
; Björkqvist, Maria
LU
; Kim, Soyon
; Jeong, Ok Chan
; Hansson, Oskar
LU
and Laurell, Thomas
LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Porous silicon, Antibody microarray, alpha-synuclein, Parkinson's, disease
- in
- Microchimica Acta
- volume
- 181
- issue
- 9-10
- pages
- 1143 - 1149
- publisher
- Springer
- external identifiers
-
- wos:000338317000034
- scopus:84903199111
- ISSN
- 1436-5073
- DOI
- 10.1007/s00604-014-1180-2
- language
- English
- LU publication?
- yes
- id
- 7ea5c63c-7b82-497d-9cda-fc7a74c1263f (old id 4602824)
- date added to LUP
- 2016-04-01 09:57:46
- date last changed
- 2023-08-30 14:19:02
@article{7ea5c63c-7b82-497d-9cda-fc7a74c1263f,
abstract = {{Levels of total and/or oligomeric alpha-synuclein may be used as a biomarker tool to aid in the diagnosis and development of new disease-modifying therapies. We report here on a porous silicon antibody microarray for the fluorimetric determination of cerebrospinal fluid levels of total alpha-synuclein, a protein involved the pathology of Parkinson's disease. The surface of porous silicon has a 3-dimensional macro- and nanoporous structure, and this offers a large binding capacity for capturing probe molecules. Porous silicon also warrants efficient immobilization of antibodies by surface adsorption, and does not require chemical immobilization. The platform requires 10 mu L of cerebrospinal fluid, and each test requires 4 h for assay only (including immobilization of capturing antibody). The limit of detection is 35 pg mL(-1) of alpha-synuclein in cerebrospinal fluid, and the dynamic analytical range extends from 0.01 to 100 ng center dot mL(-1).}},
author = {{Lee, Sangwook and Silajdzic, Edina and Yang, Hon and Björkqvist, Maria and Kim, Soyon and Jeong, Ok Chan and Hansson, Oskar and Laurell, Thomas}},
issn = {{1436-5073}},
keywords = {{Porous silicon; Antibody microarray; alpha-synuclein; Parkinson's; disease}},
language = {{eng}},
number = {{9-10}},
pages = {{1143--1149}},
publisher = {{Springer}},
series = {{Microchimica Acta}},
title = {{A porous silicon immunoassay platform for fluorometric determination of alpha-synuclein in human cerebrospinal fluid}},
url = {{http://dx.doi.org/10.1007/s00604-014-1180-2}},
doi = {{10.1007/s00604-014-1180-2}},
volume = {{181}},
year = {{2014}},
}