Phylogenetic analysis and substrate specificity of GH2 β-mannosidases from Aspergillus species.
(2013) In FEBS Letters 587(21). p.3444-3449- Abstract
- Phylogenetic analysis of glycoside hydrolase family 2 including Aspergillus sequences and characterised β-mannosidases from other organisms, clusters putative Aspergillus β-mannosidases in two distinct clades (A and B). Aspergillus species have at least one paralog in each of the two clades. It appears that clade A members are extracellular and clade B members intracellular. Substrate specificity analysis of MndA of Aspergillus niger (clade A) and MndB of Aspergillus nidulans (clade B) show that MndB, in contrast to MndA, does not hydrolyse polymeric mannan and has probably evolved to hydrolyse small unbranched β-mannosides like mannobiose. A 3D-model of MndB provides further insight.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4066123
- author
- Krishnaswamyreddy, Sumitha
LU
; Rosengren, Anna
LU
; Klaubauf, Sylvia
; Kulkarni, Tejas
LU
; Nordberg Karlsson, Eva
LU
; de Vries, Ronald P and Stålbrand, Henrik LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- FEBS Letters
- volume
- 587
- issue
- 21
- pages
- 3444 - 3449
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000325978700013
- pmid:24021641
- scopus:84886260821
- pmid:24021641
- ISSN
- 1873-3468
- DOI
- 10.1016/j.febslet.2013.08.029
- project
- Galactomannan degradation by fungi and gut bacteria
- language
- English
- LU publication?
- yes
- id
- 7f48b4fe-1cba-4097-b4cf-cd9b9f89e346 (old id 4066123)
- date added to LUP
- 2016-04-01 10:12:29
- date last changed
- 2022-01-25 20:53:53
@article{7f48b4fe-1cba-4097-b4cf-cd9b9f89e346, abstract = {{Phylogenetic analysis of glycoside hydrolase family 2 including Aspergillus sequences and characterised β-mannosidases from other organisms, clusters putative Aspergillus β-mannosidases in two distinct clades (A and B). Aspergillus species have at least one paralog in each of the two clades. It appears that clade A members are extracellular and clade B members intracellular. Substrate specificity analysis of MndA of Aspergillus niger (clade A) and MndB of Aspergillus nidulans (clade B) show that MndB, in contrast to MndA, does not hydrolyse polymeric mannan and has probably evolved to hydrolyse small unbranched β-mannosides like mannobiose. A 3D-model of MndB provides further insight.}}, author = {{Krishnaswamyreddy, Sumitha and Rosengren, Anna and Klaubauf, Sylvia and Kulkarni, Tejas and Nordberg Karlsson, Eva and de Vries, Ronald P and Stålbrand, Henrik}}, issn = {{1873-3468}}, language = {{eng}}, number = {{21}}, pages = {{3444--3449}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Letters}}, title = {{Phylogenetic analysis and substrate specificity of GH2 β-mannosidases from Aspergillus species.}}, url = {{http://dx.doi.org/10.1016/j.febslet.2013.08.029}}, doi = {{10.1016/j.febslet.2013.08.029}}, volume = {{587}}, year = {{2013}}, }