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Phylogenetic analysis and substrate specificity of GH2 β-mannosidases from Aspergillus species.

Krishnaswamyreddy, Sumitha LU ; Rosengren, Anna LU ; Klaubauf, Sylvia ; Kulkarni, Tejas LU ; Nordberg Karlsson, Eva LU orcid ; de Vries, Ronald P and Stålbrand, Henrik LU (2013) In FEBS Letters 587(21). p.3444-3449
Abstract
Phylogenetic analysis of glycoside hydrolase family 2 including Aspergillus sequences and characterised β-mannosidases from other organisms, clusters putative Aspergillus β-mannosidases in two distinct clades (A and B). Aspergillus species have at least one paralog in each of the two clades. It appears that clade A members are extracellular and clade B members intracellular. Substrate specificity analysis of MndA of Aspergillus niger (clade A) and MndB of Aspergillus nidulans (clade B) show that MndB, in contrast to MndA, does not hydrolyse polymeric mannan and has probably evolved to hydrolyse small unbranched β-mannosides like mannobiose. A 3D-model of MndB provides further insight.
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
FEBS Letters
volume
587
issue
21
pages
3444 - 3449
publisher
Wiley-Blackwell
external identifiers
  • wos:000325978700013
  • pmid:24021641
  • scopus:84886260821
  • pmid:24021641
ISSN
1873-3468
DOI
10.1016/j.febslet.2013.08.029
project
Galactomannan degradation by fungi and gut bacteria
language
English
LU publication?
yes
id
7f48b4fe-1cba-4097-b4cf-cd9b9f89e346 (old id 4066123)
date added to LUP
2016-04-01 10:12:29
date last changed
2022-01-25 20:53:53
@article{7f48b4fe-1cba-4097-b4cf-cd9b9f89e346,
  abstract     = {{Phylogenetic analysis of glycoside hydrolase family 2 including Aspergillus sequences and characterised β-mannosidases from other organisms, clusters putative Aspergillus β-mannosidases in two distinct clades (A and B). Aspergillus species have at least one paralog in each of the two clades. It appears that clade A members are extracellular and clade B members intracellular. Substrate specificity analysis of MndA of Aspergillus niger (clade A) and MndB of Aspergillus nidulans (clade B) show that MndB, in contrast to MndA, does not hydrolyse polymeric mannan and has probably evolved to hydrolyse small unbranched β-mannosides like mannobiose. A 3D-model of MndB provides further insight.}},
  author       = {{Krishnaswamyreddy, Sumitha and Rosengren, Anna and Klaubauf, Sylvia and Kulkarni, Tejas and Nordberg Karlsson, Eva and de Vries, Ronald P and Stålbrand, Henrik}},
  issn         = {{1873-3468}},
  language     = {{eng}},
  number       = {{21}},
  pages        = {{3444--3449}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{FEBS Letters}},
  title        = {{Phylogenetic analysis and substrate specificity of GH2 β-mannosidases from Aspergillus species.}},
  url          = {{http://dx.doi.org/10.1016/j.febslet.2013.08.029}},
  doi          = {{10.1016/j.febslet.2013.08.029}},
  volume       = {{587}},
  year         = {{2013}},
}