Measurement of apolipoprotein E (apoE) in cerebrospinal fluid
(2000) In Neurochemical Research 25(4). p.511-517- Abstract
- Apolipoprotein E (apoE) is a protein involved in transport of lipids and has been implicated to play an important role in regeneration after nerve injury. Determination of apoE in cerebrospinal fluid (CSF) thus have a potential interest when studying different forms of brain damage and as a marker of ongoing regenerative processes in the brain. However, previous studies on CSF-ApoE in Alzheimer's disease (AD) have given inconclusive results. Such inconsistent results might be related to confounding factors interfering with sample handling and/or analyses, which have not been fully elucidated. We therefore examined different potential confounding factors for analyses of apoE in CSF and also developed a new enzyme linked immunosorbent assay... (More)
- Apolipoprotein E (apoE) is a protein involved in transport of lipids and has been implicated to play an important role in regeneration after nerve injury. Determination of apoE in cerebrospinal fluid (CSF) thus have a potential interest when studying different forms of brain damage and as a marker of ongoing regenerative processes in the brain. However, previous studies on CSF-ApoE in Alzheimer's disease (AD) have given inconclusive results. Such inconsistent results might be related to confounding factors interfering with sample handling and/or analyses, which have not been fully elucidated. We therefore examined different potential confounding factors for analyses of apoE in CSF and also developed a new enzyme linked immunosorbent assay (ELISA). The hydrophobic character of ApoE resulted in adsorption to different types of test tubes commonly used for collection of CSF at lumbar puncture, resulting in falsely low levels. This makes CSF handling critical, especially if samples are taken in different types of tubes, or is transferred to new tubes. Taking this confounding factors in consideration and analysing patient and control CSF handled in the same way and using the new ELISA, we could confirm our previous finding of reduced levels of ApoE in AD, (3.4 +/- 1.3 mg/l) compared with controls (4.5 +/- 2.7 mg/l) (p = 0.045). Both in the AD and in the control group, higher levels of CSF-ApoE was found in individuals possessing the ApoE4 alleles. Our results support that CSF-ApoE is reduced in AD, and that handling of CSF is a critical factor, which may explain the discrepant results from previous studies. Differences in the amount of patients and controls possessing the ApoE4 allele included might also increase the variance between different studies. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1296886
- author
- Hesse, C ; Larsson, H ; Fredman, P ; Minthon, Lennart LU ; Andreasen, N ; Davidsson, P and Blennow, K
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Neurochemical Research
- volume
- 25
- issue
- 4
- pages
- 511 - 517
- publisher
- Springer
- external identifiers
-
- wos:000086991500011
- scopus:0034029839
- ISSN
- 1573-6903
- DOI
- 10.1023/A:1007516210548
- language
- English
- LU publication?
- yes
- id
- 7fb43502-0c38-48b8-ae8a-1ddef7b55b76 (old id 1296886)
- date added to LUP
- 2016-04-01 16:36:45
- date last changed
- 2022-02-20 07:11:01
@article{7fb43502-0c38-48b8-ae8a-1ddef7b55b76,
abstract = {{Apolipoprotein E (apoE) is a protein involved in transport of lipids and has been implicated to play an important role in regeneration after nerve injury. Determination of apoE in cerebrospinal fluid (CSF) thus have a potential interest when studying different forms of brain damage and as a marker of ongoing regenerative processes in the brain. However, previous studies on CSF-ApoE in Alzheimer's disease (AD) have given inconclusive results. Such inconsistent results might be related to confounding factors interfering with sample handling and/or analyses, which have not been fully elucidated. We therefore examined different potential confounding factors for analyses of apoE in CSF and also developed a new enzyme linked immunosorbent assay (ELISA). The hydrophobic character of ApoE resulted in adsorption to different types of test tubes commonly used for collection of CSF at lumbar puncture, resulting in falsely low levels. This makes CSF handling critical, especially if samples are taken in different types of tubes, or is transferred to new tubes. Taking this confounding factors in consideration and analysing patient and control CSF handled in the same way and using the new ELISA, we could confirm our previous finding of reduced levels of ApoE in AD, (3.4 +/- 1.3 mg/l) compared with controls (4.5 +/- 2.7 mg/l) (p = 0.045). Both in the AD and in the control group, higher levels of CSF-ApoE was found in individuals possessing the ApoE4 alleles. Our results support that CSF-ApoE is reduced in AD, and that handling of CSF is a critical factor, which may explain the discrepant results from previous studies. Differences in the amount of patients and controls possessing the ApoE4 allele included might also increase the variance between different studies.}},
author = {{Hesse, C and Larsson, H and Fredman, P and Minthon, Lennart and Andreasen, N and Davidsson, P and Blennow, K}},
issn = {{1573-6903}},
language = {{eng}},
number = {{4}},
pages = {{511--517}},
publisher = {{Springer}},
series = {{Neurochemical Research}},
title = {{Measurement of apolipoprotein E (apoE) in cerebrospinal fluid}},
url = {{http://dx.doi.org/10.1023/A:1007516210548}},
doi = {{10.1023/A:1007516210548}},
volume = {{25}},
year = {{2000}},
}