Advanced

Oxidative damage of DNA in subjects occupationally exposed to lead

Pawlas, Natalia; Olewinska, Elzbieta; Markiewicz-Górka, Iwona; Kozlowska, Agnieszka; Januszewska, Lidia; Lundh, Thomas LU ; Januszewska, Ewa and Pawlas, Krystyna (2017) In Advances in Clinical and Experimental Medicine 26(6). p.939-945
Abstract

Background. Exposure to lead (Pb) in environmental and occupational settings continues to be a serious public health problem and may pose an elevated risk of genetic damage. Objectives. The aim of this study was to assess the level of oxidative stress and DNA damage in subjects occupationally exposed to lead. Material and methods. We studied a population of 78 male workers exposed to lead in a lead and zinc smelter and battery recycling plant and 38 men from a control group. Blood lead levels were detected by graphite furnace atomic absorption spectrophotometry and plasma lead levels by inductively coupled plasma-mass spectrometry. The following assays were performed to assess the DNA damage and oxidative stress: comet assay,... (More)

Background. Exposure to lead (Pb) in environmental and occupational settings continues to be a serious public health problem and may pose an elevated risk of genetic damage. Objectives. The aim of this study was to assess the level of oxidative stress and DNA damage in subjects occupationally exposed to lead. Material and methods. We studied a population of 78 male workers exposed to lead in a lead and zinc smelter and battery recycling plant and 38 men from a control group. Blood lead levels were detected by graphite furnace atomic absorption spectrophotometry and plasma lead levels by inductively coupled plasma-mass spectrometry. The following assays were performed to assess the DNA damage and oxidative stress: comet assay, determination of 8-hydroxy-2'-deoxyguanosine (8-OHdG), lipid peroxidation and total antioxidant status (TAS). Results. The mean concentration of lead in the blood of the exposed group was 392 ± 103 μg/L and was significantly higher than in the control group (30.3 ± 29.4 μg/L, p < 0.0001). Oxidative DNA damages measured by comet assay showed no significant differences between populations. The concentration of 8-OHdG was about twice as high as in the control group. We found a significant positive correlation between the level of biomarkers of lead exposure [lead in blood, lead in plasma, zinc protoporphyrin (ZPP)] and urine concentration of 8-OHdG. The level of oxidative damage of DNA was positively correlated with the level of lipid peroxidation (TBARS) and negatively with total anti-oxidative status (TAS). Conclusions. Our study suggests that occupational exposure causes an increase in oxidative damage to DNA, even in subjects with relatively short length of service (average length of about 10 years). 8-OHdG concentration in the urine proved to be a sensitive and non-invasive marker of lead induced genotoxic damage.

(Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
8-hydroxy-2'-deoxyguanosine, Comet assay, DNA damage, Lead exposure, Oxidative stress
in
Advances in Clinical and Experimental Medicine
volume
26
issue
6
pages
7 pages
publisher
Wroclaw Medical University
external identifiers
  • scopus:85031049004
ISSN
1899-5276
DOI
10.17219/acem/64682
language
English
LU publication?
no
id
803103e2-f069-43af-a5a9-3b9468919908
date added to LUP
2017-10-27 08:09:07
date last changed
2018-01-07 12:23:45
@article{803103e2-f069-43af-a5a9-3b9468919908,
  abstract     = {<p>Background. Exposure to lead (Pb) in environmental and occupational settings continues to be a serious public health problem and may pose an elevated risk of genetic damage. Objectives. The aim of this study was to assess the level of oxidative stress and DNA damage in subjects occupationally exposed to lead. Material and methods. We studied a population of 78 male workers exposed to lead in a lead and zinc smelter and battery recycling plant and 38 men from a control group. Blood lead levels were detected by graphite furnace atomic absorption spectrophotometry and plasma lead levels by inductively coupled plasma-mass spectrometry. The following assays were performed to assess the DNA damage and oxidative stress: comet assay, determination of 8-hydroxy-2'-deoxyguanosine (8-OHdG), lipid peroxidation and total antioxidant status (TAS). Results. The mean concentration of lead in the blood of the exposed group was 392 ± 103 μg/L and was significantly higher than in the control group (30.3 ± 29.4 μg/L, p &lt; 0.0001). Oxidative DNA damages measured by comet assay showed no significant differences between populations. The concentration of 8-OHdG was about twice as high as in the control group. We found a significant positive correlation between the level of biomarkers of lead exposure [lead in blood, lead in plasma, zinc protoporphyrin (ZPP)] and urine concentration of 8-OHdG. The level of oxidative damage of DNA was positively correlated with the level of lipid peroxidation (TBARS) and negatively with total anti-oxidative status (TAS). Conclusions. Our study suggests that occupational exposure causes an increase in oxidative damage to DNA, even in subjects with relatively short length of service (average length of about 10 years). 8-OHdG concentration in the urine proved to be a sensitive and non-invasive marker of lead induced genotoxic damage.</p>},
  author       = {Pawlas, Natalia and Olewinska, Elzbieta and Markiewicz-Górka, Iwona and Kozlowska, Agnieszka and Januszewska, Lidia and Lundh, Thomas and Januszewska, Ewa and Pawlas, Krystyna},
  issn         = {1899-5276},
  keyword      = {8-hydroxy-2'-deoxyguanosine,Comet assay,DNA damage,Lead exposure,Oxidative stress},
  language     = {eng},
  month        = {09},
  number       = {6},
  pages        = {939--945},
  publisher    = {Wroclaw Medical University},
  series       = {Advances in Clinical and Experimental Medicine},
  title        = {Oxidative damage of DNA in subjects occupationally exposed to lead},
  url          = {http://dx.doi.org/10.17219/acem/64682},
  volume       = {26},
  year         = {2017},
}