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Comprehensive analysis of microRNA expression in regionalized human neural progenitor cells reveals microRNA-10 as a caudalizing factor.

Jönsson, Marie LU ; Nelander Wahlestedt, Jenny LU ; Åkerblom, Malin LU ; Kirkeby, Agnete LU ; Malmevik, Josephine LU ; Brattaas, Per Ludvik LU ; Jakobsson, Johan LU and Parmar, Malin LU (2015) In Development 142(18). p.3166-3177
Abstract
MicroRNAs (miRNAs) have been implicated in regulating multiple processes during brain development in various species. However, the function of miRNAs in human brain development remains largely unexplored. Here, we provide a comprehensive analysis of miRNA expression of regionalized neural progenitor cells derived from human embryonic stem cells and human foetal brain. We found miR-92b-3p and miR-130b-5p to be specifically associated with neural progenitors and several miRNAs that display both age-specific and region-specific expression patterns. Among these miRNAs, we identified miR-10 to be specifically expressed in the human hindbrain and spinal cord, while being absent from rostral regions. We found that miR-10 regulates a large number... (More)
MicroRNAs (miRNAs) have been implicated in regulating multiple processes during brain development in various species. However, the function of miRNAs in human brain development remains largely unexplored. Here, we provide a comprehensive analysis of miRNA expression of regionalized neural progenitor cells derived from human embryonic stem cells and human foetal brain. We found miR-92b-3p and miR-130b-5p to be specifically associated with neural progenitors and several miRNAs that display both age-specific and region-specific expression patterns. Among these miRNAs, we identified miR-10 to be specifically expressed in the human hindbrain and spinal cord, while being absent from rostral regions. We found that miR-10 regulates a large number of genes enriched for functions including transcription, actin cytoskeleton and ephrin receptor signalling. When overexpressed, miR-10 influences caudalization of human neural progenitor cells. Together, these data confirm a role for miRNAs in establishing different human neural progenitor populations. This dataset also provides a comprehensive resource for future studies investigating the functional role of different miRNAs in human brain development. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Development
volume
142
issue
18
pages
3166 - 3177
publisher
Society for International Development
external identifiers
  • pmid:26395143
  • wos:000361742600011
  • scopus:84942163494
ISSN
1477-9129
DOI
10.1242/dev.122747
language
English
LU publication?
yes
id
27f1747e-77b4-4223-a7bf-5e303ef38341 (old id 8035305)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26395143?dopt=Abstract
date added to LUP
2015-10-03 17:09:35
date last changed
2017-11-05 03:14:08
@article{27f1747e-77b4-4223-a7bf-5e303ef38341,
  abstract     = {MicroRNAs (miRNAs) have been implicated in regulating multiple processes during brain development in various species. However, the function of miRNAs in human brain development remains largely unexplored. Here, we provide a comprehensive analysis of miRNA expression of regionalized neural progenitor cells derived from human embryonic stem cells and human foetal brain. We found miR-92b-3p and miR-130b-5p to be specifically associated with neural progenitors and several miRNAs that display both age-specific and region-specific expression patterns. Among these miRNAs, we identified miR-10 to be specifically expressed in the human hindbrain and spinal cord, while being absent from rostral regions. We found that miR-10 regulates a large number of genes enriched for functions including transcription, actin cytoskeleton and ephrin receptor signalling. When overexpressed, miR-10 influences caudalization of human neural progenitor cells. Together, these data confirm a role for miRNAs in establishing different human neural progenitor populations. This dataset also provides a comprehensive resource for future studies investigating the functional role of different miRNAs in human brain development.},
  author       = {Jönsson, Marie and Nelander Wahlestedt, Jenny and Åkerblom, Malin and Kirkeby, Agnete and Malmevik, Josephine and Brattaas, Per Ludvik and Jakobsson, Johan and Parmar, Malin},
  issn         = {1477-9129},
  language     = {eng},
  number       = {18},
  pages        = {3166--3177},
  publisher    = {Society for International Development},
  series       = {Development},
  title        = {Comprehensive analysis of microRNA expression in regionalized human neural progenitor cells reveals microRNA-10 as a caudalizing factor.},
  url          = {http://dx.doi.org/10.1242/dev.122747},
  volume       = {142},
  year         = {2015},
}