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Altered Immunogenicity of Donor Lungs via Removal of Passenger Leukocytes Using Ex Vivo Lung Perfusion.

Stone, J P; Critchley, W R; Major, T; Rajan, G; Risnes, I; Scott, H; Liao, Qiuming LU ; Wohlfart, Björn LU ; Sjöberg, Trygve LU and Yonan, N, et al. (2016) In American Journal of Transplantation 16(1). p.33-43
Abstract
Passenger leukocyte transfer from the donor lung to the recipient is intrinsically involved in acute rejection. Direct presentation of alloantigen expressed on donor leukocytes is recognized by recipient T cells, promoting acute cellular rejection. We utilized ex vivo lung perfusion (EVLP) to study passenger leukocyte migration from donor lungs into the recipient and to evaluate the effects of donor leukocyte depletion prior to transplantation. For this purpose, female pigs received male left lungs either following 3 h of EVLP or retrieved using standard protocols. Recipients were monitored for 24 h and sequential samples were collected. EVLP-reduced donor leukocyte transfer into the recipient and migration to recipient lymph nodes was... (More)
Passenger leukocyte transfer from the donor lung to the recipient is intrinsically involved in acute rejection. Direct presentation of alloantigen expressed on donor leukocytes is recognized by recipient T cells, promoting acute cellular rejection. We utilized ex vivo lung perfusion (EVLP) to study passenger leukocyte migration from donor lungs into the recipient and to evaluate the effects of donor leukocyte depletion prior to transplantation. For this purpose, female pigs received male left lungs either following 3 h of EVLP or retrieved using standard protocols. Recipients were monitored for 24 h and sequential samples were collected. EVLP-reduced donor leukocyte transfer into the recipient and migration to recipient lymph nodes was markedly reduced. Recipient T cell infiltration of the donor lung was significantly diminished via EVLP. Donor leukocyte removal during EVLP reduces direct allorecognition and T cell priming, diminishing recipient T cell infiltration, the hallmark of acute rejection. (Less)
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Contribution to journal
publication status
published
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in
American Journal of Transplantation
volume
16
issue
1
pages
33 - 43
publisher
Wiley-Blackwell
external identifiers
  • pmid:26366523
  • wos:000368507300010
  • scopus:84956873967
ISSN
1600-6135
DOI
10.1111/ajt.13446
language
English
LU publication?
yes
id
90dc2726-c019-4026-a924-5ad0b057a16e (old id 8042378)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26366523?dopt=Abstract
date added to LUP
2015-10-04 13:31:14
date last changed
2017-11-05 03:24:41
@article{90dc2726-c019-4026-a924-5ad0b057a16e,
  abstract     = {Passenger leukocyte transfer from the donor lung to the recipient is intrinsically involved in acute rejection. Direct presentation of alloantigen expressed on donor leukocytes is recognized by recipient T cells, promoting acute cellular rejection. We utilized ex vivo lung perfusion (EVLP) to study passenger leukocyte migration from donor lungs into the recipient and to evaluate the effects of donor leukocyte depletion prior to transplantation. For this purpose, female pigs received male left lungs either following 3 h of EVLP or retrieved using standard protocols. Recipients were monitored for 24 h and sequential samples were collected. EVLP-reduced donor leukocyte transfer into the recipient and migration to recipient lymph nodes was markedly reduced. Recipient T cell infiltration of the donor lung was significantly diminished via EVLP. Donor leukocyte removal during EVLP reduces direct allorecognition and T cell priming, diminishing recipient T cell infiltration, the hallmark of acute rejection.},
  author       = {Stone, J P and Critchley, W R and Major, T and Rajan, G and Risnes, I and Scott, H and Liao, Qiuming and Wohlfart, Björn and Sjöberg, Trygve and Yonan, N and Steen, Stig and Fildes, J E},
  issn         = {1600-6135},
  language     = {eng},
  number       = {1},
  pages        = {33--43},
  publisher    = {Wiley-Blackwell},
  series       = {American Journal of Transplantation},
  title        = {Altered Immunogenicity of Donor Lungs via Removal of Passenger Leukocytes Using Ex Vivo Lung Perfusion.},
  url          = {http://dx.doi.org/10.1111/ajt.13446},
  volume       = {16},
  year         = {2016},
}