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Nerve regeneration in chitosan conduits and in autologous nerve grafts in healthy and in type 2 diabetic Goto-Kakizaki rats.

Stenberg, Lena LU ; Kodama, Akira; Lindwall-Blom, Charlotta and Dahlin, Lars LU (2016) In European Journal of Neuroscience 43(3). p.73-463
Abstract
Knowledge about nerve regeneration after nerve injury and reconstruction in appropriate diabetic animal models is incomplete. Short-term nerve regeneration after reconstruction of a 10 mm sciatic nerve defect with either a hollow chitosan conduit or an autologous nerve graft was investigated in healthy Wistar and diabetic Goto-Kakizaki (GK) rats. After 21 days, axonal outgrowth, presence of activated and apoptotic Schwann cells, as well as thickness of the formed matrix in the conduits were measured. In general, nerve regeneration was superior in autologous nerve grafts. In chitosan conduits, a matrix, which was thicker in diabetic rats, was formed and was positively correlated with length of axonal outgrowth. Axonal outgrowth in conduits... (More)
Knowledge about nerve regeneration after nerve injury and reconstruction in appropriate diabetic animal models is incomplete. Short-term nerve regeneration after reconstruction of a 10 mm sciatic nerve defect with either a hollow chitosan conduit or an autologous nerve graft was investigated in healthy Wistar and diabetic Goto-Kakizaki (GK) rats. After 21 days, axonal outgrowth, presence of activated and apoptotic Schwann cells, as well as thickness of the formed matrix in the conduits were measured. In general, nerve regeneration was superior in autologous nerve grafts. In chitosan conduits, a matrix, which was thicker in diabetic rats, was formed and was positively correlated with length of axonal outgrowth. Axonal outgrowth in conduits and in nerve grafts extended further in diabetic rats compared to healthy rats. There was a higher percentage of activating transcription factor 3 (ATF-3) immunostained cells in nerve segments from healthy rats compared to diabetic rats after autologous nerve graft reconstruction. In chitosan conduits, more cleaved caspase 3 stained Schwann cells were generally observed in the matrix from the diabetic rats compared to healthy rats. However, there were fewer apoptotic cells in the distal segment in diabetic rats reconstructed with a chitosan conduit. Preoperative glucose levels were positively correlated with axonal outgrowth after both reconstruction mehods. Axonal regeneration was better in autologous nerve grafts compared to hollow chitosan conduits and was enhanced in diabetic GK rats compared to healthy rats after reconstruction. This study provides insights into the nerve regeneration process in a clinically relevant diabetic animal model. This article is protected by copyright. All rights reserved. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
European Journal of Neuroscience
volume
43
issue
3
pages
73 - 463
publisher
Wiley-Blackwell
external identifiers
  • pmid:26355640
  • scopus:84956579981
  • wos:000369764500016
ISSN
1460-9568
DOI
10.1111/ejn.13068
language
English
LU publication?
yes
id
541ede7b-8d3a-45c4-b53f-a9705fe19c6d (old id 8042625)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26355640?dopt=Abstract
date added to LUP
2015-10-04 14:51:28
date last changed
2017-11-19 04:19:05
@article{541ede7b-8d3a-45c4-b53f-a9705fe19c6d,
  abstract     = {Knowledge about nerve regeneration after nerve injury and reconstruction in appropriate diabetic animal models is incomplete. Short-term nerve regeneration after reconstruction of a 10 mm sciatic nerve defect with either a hollow chitosan conduit or an autologous nerve graft was investigated in healthy Wistar and diabetic Goto-Kakizaki (GK) rats. After 21 days, axonal outgrowth, presence of activated and apoptotic Schwann cells, as well as thickness of the formed matrix in the conduits were measured. In general, nerve regeneration was superior in autologous nerve grafts. In chitosan conduits, a matrix, which was thicker in diabetic rats, was formed and was positively correlated with length of axonal outgrowth. Axonal outgrowth in conduits and in nerve grafts extended further in diabetic rats compared to healthy rats. There was a higher percentage of activating transcription factor 3 (ATF-3) immunostained cells in nerve segments from healthy rats compared to diabetic rats after autologous nerve graft reconstruction. In chitosan conduits, more cleaved caspase 3 stained Schwann cells were generally observed in the matrix from the diabetic rats compared to healthy rats. However, there were fewer apoptotic cells in the distal segment in diabetic rats reconstructed with a chitosan conduit. Preoperative glucose levels were positively correlated with axonal outgrowth after both reconstruction mehods. Axonal regeneration was better in autologous nerve grafts compared to hollow chitosan conduits and was enhanced in diabetic GK rats compared to healthy rats after reconstruction. This study provides insights into the nerve regeneration process in a clinically relevant diabetic animal model. This article is protected by copyright. All rights reserved.},
  author       = {Stenberg, Lena and Kodama, Akira and Lindwall-Blom, Charlotta and Dahlin, Lars},
  issn         = {1460-9568},
  language     = {eng},
  number       = {3},
  pages        = {73--463},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Neuroscience},
  title        = {Nerve regeneration in chitosan conduits and in autologous nerve grafts in healthy and in type 2 diabetic Goto-Kakizaki rats.},
  url          = {http://dx.doi.org/10.1111/ejn.13068},
  volume       = {43},
  year         = {2016},
}