Quantitative interpretation of gold nanoparticle-based bioassays designed for detection of immunocomplex formation
(2007) In Biointerphases 2(1). p.6-15- Abstract
- The authors present in this paper how the extended Mie theory can be used to translate not only end-point data but also temporal variations of extinction peak-position changes, peak(t), into absolute mass uptake, (t), upon biomacromolecule binding to localized surface plasmon resonance (SPR) active nanoparticles (NPs). The theoretical analysis is applied on a novel sensor template composed of a three-layer surface architecture based on (i) a self-assembled monolayer of HS(CH2)15COOH, (ii) a 1:1 mixture of biotinylated and pure poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG), and (iii) NeutrAvidin. Assisted by independent estimations of the thickness of the three-layer architecture using quartz crystal microbalance with dissipation... (More)
- The authors present in this paper how the extended Mie theory can be used to translate not only end-point data but also temporal variations of extinction peak-position changes, peak(t), into absolute mass uptake, (t), upon biomacromolecule binding to localized surface plasmon resonance (SPR) active nanoparticles (NPs). The theoretical analysis is applied on a novel sensor template composed of a three-layer surface architecture based on (i) a self-assembled monolayer of HS(CH2)15COOH, (ii) a 1:1 mixture of biotinylated and pure poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG), and (iii) NeutrAvidin. Assisted by independent estimations of the thickness of the three-layer architecture using quartz crystal microbalance with dissipation (QCM-D) monitoring, excellent agreement with parallel mass-uptake estimations using planar SPR is obtained. Furthermore, unspecific binding of serum to PLL-g-PEG was shown to be below the detection limit, making the surface architecture ideally suited for label-free detection of immunoreactions. To ensure that the immunocomplex formation occurred within the limited sensing depth (~10 nm) of the NPs, a compact model system composed of a biotinylated human recombinant single-chain antibody fragment (~2 nm) directed against cholera toxin was selected. By tracking changes in the centroid (center of mass) of the extinction peak, rather than the actual peak position, signal-to-noise levels and long-term stability upon cholera toxin detection are demonstrated to be competitive with results obtained using conventional SPR and state-of-the-art QCM-D data. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/809677
- author
- Zhou, Ye ; Xu, Hongxing LU ; Dahlin, Andreas LU ; Vallkil, Jacob LU ; Borrebaeck, Carl LU ; Wingren, Christer LU ; Liedberg, Bo and Höök, Fredrik LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biointerphases
- volume
- 2
- issue
- 1
- pages
- 6 - 15
- publisher
- AVS
- external identifiers
-
- wos:000256466800002
- scopus:33846820443
- pmid:20408631
- ISSN
- 1934-8630
- DOI
- 10.1116/1.2700235
- language
- English
- LU publication?
- yes
- id
- 7518cb89-c976-4b6c-83e4-c3de71f7005e (old id 809677)
- date added to LUP
- 2016-04-04 08:36:34
- date last changed
- 2024-08-20 11:37:44
@article{7518cb89-c976-4b6c-83e4-c3de71f7005e, abstract = {{The authors present in this paper how the extended Mie theory can be used to translate not only end-point data but also temporal variations of extinction peak-position changes, peak(t), into absolute mass uptake, (t), upon biomacromolecule binding to localized surface plasmon resonance (SPR) active nanoparticles (NPs). The theoretical analysis is applied on a novel sensor template composed of a three-layer surface architecture based on (i) a self-assembled monolayer of HS(CH2)15COOH, (ii) a 1:1 mixture of biotinylated and pure poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG), and (iii) NeutrAvidin. Assisted by independent estimations of the thickness of the three-layer architecture using quartz crystal microbalance with dissipation (QCM-D) monitoring, excellent agreement with parallel mass-uptake estimations using planar SPR is obtained. Furthermore, unspecific binding of serum to PLL-g-PEG was shown to be below the detection limit, making the surface architecture ideally suited for label-free detection of immunoreactions. To ensure that the immunocomplex formation occurred within the limited sensing depth (~10 nm) of the NPs, a compact model system composed of a biotinylated human recombinant single-chain antibody fragment (~2 nm) directed against cholera toxin was selected. By tracking changes in the centroid (center of mass) of the extinction peak, rather than the actual peak position, signal-to-noise levels and long-term stability upon cholera toxin detection are demonstrated to be competitive with results obtained using conventional SPR and state-of-the-art QCM-D data.}}, author = {{Zhou, Ye and Xu, Hongxing and Dahlin, Andreas and Vallkil, Jacob and Borrebaeck, Carl and Wingren, Christer and Liedberg, Bo and Höök, Fredrik}}, issn = {{1934-8630}}, language = {{eng}}, number = {{1}}, pages = {{6--15}}, publisher = {{AVS}}, series = {{Biointerphases}}, title = {{Quantitative interpretation of gold nanoparticle-based bioassays designed for detection of immunocomplex formation}}, url = {{http://dx.doi.org/10.1116/1.2700235}}, doi = {{10.1116/1.2700235}}, volume = {{2}}, year = {{2007}}, }