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Substance P-induced relaxation and hyperpolarization in human cerebral arteries

Petersson, Jesper LU ; Zygmunt, Peter M LU ; Brandt, Lennart LU and Högestätt, Edward D LU (1995) In British Journal of Pharmacology 115(6). p.94-889
Abstract

1. Vascular effects of substance P were studied in human isolated pial arteries removed from 14 patients undergoing cerebral cortical resection. 2. Substance P induced a concentration-dependent relaxation in the presence of indomethacin. No relaxation was seen in arteries where the endothelium had been removed. 3. N omega-nitro-L-arginine (L-NOARG, 0.3 mM) abolished the relaxation in arteries from six patients. The relaxation was only partially inhibited in the remaining eight patients, the reduction of the maximum relaxation being less than 50% in each patient. 4. The L-NOARG-resistant relaxation was abolished when the external K+ concentration was raised above 30 mM. 5. Substance P caused a smooth muscle hyperpolarization (in the... (More)

1. Vascular effects of substance P were studied in human isolated pial arteries removed from 14 patients undergoing cerebral cortical resection. 2. Substance P induced a concentration-dependent relaxation in the presence of indomethacin. No relaxation was seen in arteries where the endothelium had been removed. 3. N omega-nitro-L-arginine (L-NOARG, 0.3 mM) abolished the relaxation in arteries from six patients. The relaxation was only partially inhibited in the remaining eight patients, the reduction of the maximum relaxation being less than 50% in each patient. 4. The L-NOARG-resistant relaxation was abolished when the external K+ concentration was raised above 30 mM. 5. Substance P caused a smooth muscle hyperpolarization (in the presence of L-NOARG and indomethacin), but only when the artery showed an L-NOARG-resistant relaxation. 6. The results indicate that nitric oxide is an important mediator of endothelium-dependent relaxation in human cerebral arteries. Furthermore, another endothelium-dependent pathway, causing hyperpolarization and vasodilatation, was identified in arteries from more than half the population of patients.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Adult, Aged, Benzopyrans/pharmacology, Cerebral Arteries/drug effects, Cromakalim, Dose-Response Relationship, Drug, Endothelium, Vascular/drug effects, Female, Humans, Male, Membrane Potentials/drug effects, Middle Aged, Pyrroles/pharmacology, Substance P/pharmacology, Vasodilation/drug effects, Vasodilator Agents/pharmacology
in
British Journal of Pharmacology
volume
115
issue
6
pages
94 - 889
publisher
The British Pharmacological Society
external identifiers
  • scopus:0029149343
ISSN
0007-1188
DOI
10.1111/j.1476-5381.1995.tb15893.x
language
English
LU publication?
no
id
80a8fe89-197a-44fa-8570-cca6f20442ab
date added to LUP
2019-02-20 10:18:23
date last changed
2019-03-17 05:13:06
@article{80a8fe89-197a-44fa-8570-cca6f20442ab,
  abstract     = {<p>1. Vascular effects of substance P were studied in human isolated pial arteries removed from 14 patients undergoing cerebral cortical resection. 2. Substance P induced a concentration-dependent relaxation in the presence of indomethacin. No relaxation was seen in arteries where the endothelium had been removed. 3. N omega-nitro-L-arginine (L-NOARG, 0.3 mM) abolished the relaxation in arteries from six patients. The relaxation was only partially inhibited in the remaining eight patients, the reduction of the maximum relaxation being less than 50% in each patient. 4. The L-NOARG-resistant relaxation was abolished when the external K+ concentration was raised above 30 mM. 5. Substance P caused a smooth muscle hyperpolarization (in the presence of L-NOARG and indomethacin), but only when the artery showed an L-NOARG-resistant relaxation. 6. The results indicate that nitric oxide is an important mediator of endothelium-dependent relaxation in human cerebral arteries. Furthermore, another endothelium-dependent pathway, causing hyperpolarization and vasodilatation, was identified in arteries from more than half the population of patients.</p>},
  author       = {Petersson, Jesper and Zygmunt, Peter M and Brandt, Lennart and Högestätt, Edward D},
  issn         = {0007-1188},
  keyword      = {Adult,Aged,Benzopyrans/pharmacology,Cerebral Arteries/drug effects,Cromakalim,Dose-Response Relationship, Drug,Endothelium, Vascular/drug effects,Female,Humans,Male,Membrane Potentials/drug effects,Middle Aged,Pyrroles/pharmacology,Substance P/pharmacology,Vasodilation/drug effects,Vasodilator Agents/pharmacology},
  language     = {eng},
  number       = {6},
  pages        = {94--889},
  publisher    = {The British Pharmacological Society},
  series       = {British Journal of Pharmacology},
  title        = {Substance P-induced relaxation and hyperpolarization in human cerebral arteries},
  url          = {http://dx.doi.org/10.1111/j.1476-5381.1995.tb15893.x},
  volume       = {115},
  year         = {1995},
}