Substance P-induced relaxation and hyperpolarization in human cerebral arteries
(1995) In British Journal of Pharmacology 115(6). p.94-889- Abstract
1. Vascular effects of substance P were studied in human isolated pial arteries removed from 14 patients undergoing cerebral cortical resection. 2. Substance P induced a concentration-dependent relaxation in the presence of indomethacin. No relaxation was seen in arteries where the endothelium had been removed. 3. N omega-nitro-L-arginine (L-NOARG, 0.3 mM) abolished the relaxation in arteries from six patients. The relaxation was only partially inhibited in the remaining eight patients, the reduction of the maximum relaxation being less than 50% in each patient. 4. The L-NOARG-resistant relaxation was abolished when the external K+ concentration was raised above 30 mM. 5. Substance P caused a smooth muscle hyperpolarization (in the... (More)
1. Vascular effects of substance P were studied in human isolated pial arteries removed from 14 patients undergoing cerebral cortical resection. 2. Substance P induced a concentration-dependent relaxation in the presence of indomethacin. No relaxation was seen in arteries where the endothelium had been removed. 3. N omega-nitro-L-arginine (L-NOARG, 0.3 mM) abolished the relaxation in arteries from six patients. The relaxation was only partially inhibited in the remaining eight patients, the reduction of the maximum relaxation being less than 50% in each patient. 4. The L-NOARG-resistant relaxation was abolished when the external K+ concentration was raised above 30 mM. 5. Substance P caused a smooth muscle hyperpolarization (in the presence of L-NOARG and indomethacin), but only when the artery showed an L-NOARG-resistant relaxation. 6. The results indicate that nitric oxide is an important mediator of endothelium-dependent relaxation in human cerebral arteries. Furthermore, another endothelium-dependent pathway, causing hyperpolarization and vasodilatation, was identified in arteries from more than half the population of patients.
(Less)
- author
- Petersson, Jesper LU ; Zygmunt, Peter M LU ; Brandt, Lennart LU and Högestätt, Edward D LU
- publishing date
- 1995-07
- type
- Contribution to journal
- publication status
- published
- keywords
- Adult, Aged, Benzopyrans/pharmacology, Cerebral Arteries/drug effects, Cromakalim, Dose-Response Relationship, Drug, Endothelium, Vascular/drug effects, Female, Humans, Male, Membrane Potentials/drug effects, Middle Aged, Pyrroles/pharmacology, Substance P/pharmacology, Vasodilation/drug effects, Vasodilator Agents/pharmacology
- in
- British Journal of Pharmacology
- volume
- 115
- issue
- 6
- pages
- 94 - 889
- publisher
- Wiley
- external identifiers
-
- pmid:7582516
- scopus:0029149343
- ISSN
- 0007-1188
- DOI
- 10.1111/j.1476-5381.1995.tb15893.x
- language
- English
- LU publication?
- no
- id
- 80a8fe89-197a-44fa-8570-cca6f20442ab
- date added to LUP
- 2019-02-20 10:18:23
- date last changed
- 2024-09-04 12:42:37
@article{80a8fe89-197a-44fa-8570-cca6f20442ab, abstract = {{<p>1. Vascular effects of substance P were studied in human isolated pial arteries removed from 14 patients undergoing cerebral cortical resection. 2. Substance P induced a concentration-dependent relaxation in the presence of indomethacin. No relaxation was seen in arteries where the endothelium had been removed. 3. N omega-nitro-L-arginine (L-NOARG, 0.3 mM) abolished the relaxation in arteries from six patients. The relaxation was only partially inhibited in the remaining eight patients, the reduction of the maximum relaxation being less than 50% in each patient. 4. The L-NOARG-resistant relaxation was abolished when the external K+ concentration was raised above 30 mM. 5. Substance P caused a smooth muscle hyperpolarization (in the presence of L-NOARG and indomethacin), but only when the artery showed an L-NOARG-resistant relaxation. 6. The results indicate that nitric oxide is an important mediator of endothelium-dependent relaxation in human cerebral arteries. Furthermore, another endothelium-dependent pathway, causing hyperpolarization and vasodilatation, was identified in arteries from more than half the population of patients.</p>}}, author = {{Petersson, Jesper and Zygmunt, Peter M and Brandt, Lennart and Högestätt, Edward D}}, issn = {{0007-1188}}, keywords = {{Adult; Aged; Benzopyrans/pharmacology; Cerebral Arteries/drug effects; Cromakalim; Dose-Response Relationship, Drug; Endothelium, Vascular/drug effects; Female; Humans; Male; Membrane Potentials/drug effects; Middle Aged; Pyrroles/pharmacology; Substance P/pharmacology; Vasodilation/drug effects; Vasodilator Agents/pharmacology}}, language = {{eng}}, number = {{6}}, pages = {{94--889}}, publisher = {{Wiley}}, series = {{British Journal of Pharmacology}}, title = {{Substance P-induced relaxation and hyperpolarization in human cerebral arteries}}, url = {{http://dx.doi.org/10.1111/j.1476-5381.1995.tb15893.x}}, doi = {{10.1111/j.1476-5381.1995.tb15893.x}}, volume = {{115}}, year = {{1995}}, }