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Substance P-induced relaxation and hyperpolarization in human cerebral arteries

Petersson, Jesper LU ; Zygmunt, Peter M LU orcid ; Brandt, Lennart LU and Högestätt, Edward D LU (1995) In British Journal of Pharmacology 115(6). p.94-889
Abstract

1. Vascular effects of substance P were studied in human isolated pial arteries removed from 14 patients undergoing cerebral cortical resection. 2. Substance P induced a concentration-dependent relaxation in the presence of indomethacin. No relaxation was seen in arteries where the endothelium had been removed. 3. N omega-nitro-L-arginine (L-NOARG, 0.3 mM) abolished the relaxation in arteries from six patients. The relaxation was only partially inhibited in the remaining eight patients, the reduction of the maximum relaxation being less than 50% in each patient. 4. The L-NOARG-resistant relaxation was abolished when the external K+ concentration was raised above 30 mM. 5. Substance P caused a smooth muscle hyperpolarization (in the... (More)

1. Vascular effects of substance P were studied in human isolated pial arteries removed from 14 patients undergoing cerebral cortical resection. 2. Substance P induced a concentration-dependent relaxation in the presence of indomethacin. No relaxation was seen in arteries where the endothelium had been removed. 3. N omega-nitro-L-arginine (L-NOARG, 0.3 mM) abolished the relaxation in arteries from six patients. The relaxation was only partially inhibited in the remaining eight patients, the reduction of the maximum relaxation being less than 50% in each patient. 4. The L-NOARG-resistant relaxation was abolished when the external K+ concentration was raised above 30 mM. 5. Substance P caused a smooth muscle hyperpolarization (in the presence of L-NOARG and indomethacin), but only when the artery showed an L-NOARG-resistant relaxation. 6. The results indicate that nitric oxide is an important mediator of endothelium-dependent relaxation in human cerebral arteries. Furthermore, another endothelium-dependent pathway, causing hyperpolarization and vasodilatation, was identified in arteries from more than half the population of patients.

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author
; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Adult, Aged, Benzopyrans/pharmacology, Cerebral Arteries/drug effects, Cromakalim, Dose-Response Relationship, Drug, Endothelium, Vascular/drug effects, Female, Humans, Male, Membrane Potentials/drug effects, Middle Aged, Pyrroles/pharmacology, Substance P/pharmacology, Vasodilation/drug effects, Vasodilator Agents/pharmacology
in
British Journal of Pharmacology
volume
115
issue
6
pages
94 - 889
publisher
Wiley
external identifiers
  • pmid:7582516
  • scopus:0029149343
ISSN
0007-1188
DOI
10.1111/j.1476-5381.1995.tb15893.x
language
English
LU publication?
no
id
80a8fe89-197a-44fa-8570-cca6f20442ab
date added to LUP
2019-02-20 10:18:23
date last changed
2025-10-14 10:00:33
@article{80a8fe89-197a-44fa-8570-cca6f20442ab,
  abstract     = {{<p>1. Vascular effects of substance P were studied in human isolated pial arteries removed from 14 patients undergoing cerebral cortical resection. 2. Substance P induced a concentration-dependent relaxation in the presence of indomethacin. No relaxation was seen in arteries where the endothelium had been removed. 3. N omega-nitro-L-arginine (L-NOARG, 0.3 mM) abolished the relaxation in arteries from six patients. The relaxation was only partially inhibited in the remaining eight patients, the reduction of the maximum relaxation being less than 50% in each patient. 4. The L-NOARG-resistant relaxation was abolished when the external K+ concentration was raised above 30 mM. 5. Substance P caused a smooth muscle hyperpolarization (in the presence of L-NOARG and indomethacin), but only when the artery showed an L-NOARG-resistant relaxation. 6. The results indicate that nitric oxide is an important mediator of endothelium-dependent relaxation in human cerebral arteries. Furthermore, another endothelium-dependent pathway, causing hyperpolarization and vasodilatation, was identified in arteries from more than half the population of patients.</p>}},
  author       = {{Petersson, Jesper and Zygmunt, Peter M and Brandt, Lennart and Högestätt, Edward D}},
  issn         = {{0007-1188}},
  keywords     = {{Adult; Aged; Benzopyrans/pharmacology; Cerebral Arteries/drug effects; Cromakalim; Dose-Response Relationship, Drug; Endothelium, Vascular/drug effects; Female; Humans; Male; Membrane Potentials/drug effects; Middle Aged; Pyrroles/pharmacology; Substance P/pharmacology; Vasodilation/drug effects; Vasodilator Agents/pharmacology}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{94--889}},
  publisher    = {{Wiley}},
  series       = {{British Journal of Pharmacology}},
  title        = {{Substance P-induced relaxation and hyperpolarization in human cerebral arteries}},
  url          = {{http://dx.doi.org/10.1111/j.1476-5381.1995.tb15893.x}},
  doi          = {{10.1111/j.1476-5381.1995.tb15893.x}},
  volume       = {{115}},
  year         = {{1995}},
}