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Improved production of 3-Hydroxypropionaldehyde by complex formation with bisulfite during biotransformation of glycerol

Sardari, Roya LU ; Dishisha, Tarek LU ; Pyo, Sang-Hyun LU and Hatti-Kaul, Rajni LU (2013) In Biotechnology and Bioengineering 110(4). p.1243-1248
Abstract
3-Hydroxypropionaldehyde (3HPA) is an important specialty chemical which can be produced from glycerol using resting cells of Lactobacillus reuteri. This biocatalytic route, however, suffers from substrate- and product-mediated loss of enzyme activity within 2 h of biotransformation. In order to overcome the inhibitory effects of 3HPA, complex formation with sodium bisulfite was investigated, optimized and applied for in situ capture of the aldehyde during biotransformation of glycerol in a fed-batch process. As a result, the activity of the cells was maintained for at least 18 h. The 3HPA produced per gram cell dry weight was increased 5.7 times compared to the batch production process, and 2.2 times compared to fed-batch process without... (More)
3-Hydroxypropionaldehyde (3HPA) is an important specialty chemical which can be produced from glycerol using resting cells of Lactobacillus reuteri. This biocatalytic route, however, suffers from substrate- and product-mediated loss of enzyme activity within 2 h of biotransformation. In order to overcome the inhibitory effects of 3HPA, complex formation with sodium bisulfite was investigated, optimized and applied for in situ capture of the aldehyde during biotransformation of glycerol in a fed-batch process. As a result, the activity of the cells was maintained for at least 18 h. The 3HPA produced per gram cell dry weight was increased 5.7 times compared to the batch production process, and 2.2 times compared to fed-batch process without in situ complex formation. This approach may have potential for production and in situ removal of 3HPA after further process development. Biotechnol. Bioeng. © 2012 Wiley Periodicals, Inc. (Less)
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; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology and Bioengineering
volume
110
issue
4
pages
1243 - 1248
publisher
John Wiley & Sons Inc.
external identifiers
  • wos:000315360100024
  • pmid:23172314
  • scopus:84874211159
  • pmid:23172314
ISSN
1097-0290
DOI
10.1002/bit.24787
language
English
LU publication?
yes
id
80b990b7-21dc-466c-9645-daadf4408b40 (old id 3218605)
date added to LUP
2016-04-01 10:53:43
date last changed
2024-01-07 03:54:06
@article{80b990b7-21dc-466c-9645-daadf4408b40,
  abstract     = {{3-Hydroxypropionaldehyde (3HPA) is an important specialty chemical which can be produced from glycerol using resting cells of Lactobacillus reuteri. This biocatalytic route, however, suffers from substrate- and product-mediated loss of enzyme activity within 2 h of biotransformation. In order to overcome the inhibitory effects of 3HPA, complex formation with sodium bisulfite was investigated, optimized and applied for in situ capture of the aldehyde during biotransformation of glycerol in a fed-batch process. As a result, the activity of the cells was maintained for at least 18 h. The 3HPA produced per gram cell dry weight was increased 5.7 times compared to the batch production process, and 2.2 times compared to fed-batch process without in situ complex formation. This approach may have potential for production and in situ removal of 3HPA after further process development. Biotechnol. Bioeng. © 2012 Wiley Periodicals, Inc.}},
  author       = {{Sardari, Roya and Dishisha, Tarek and Pyo, Sang-Hyun and Hatti-Kaul, Rajni}},
  issn         = {{1097-0290}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{1243--1248}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Biotechnology and Bioengineering}},
  title        = {{Improved production of 3-Hydroxypropionaldehyde by complex formation with bisulfite during biotransformation of glycerol}},
  url          = {{http://dx.doi.org/10.1002/bit.24787}},
  doi          = {{10.1002/bit.24787}},
  volume       = {{110}},
  year         = {{2013}},
}