Improved production of 3-Hydroxypropionaldehyde by complex formation with bisulfite during biotransformation of glycerol
(2013) In Biotechnology and Bioengineering 110(4). p.1243-1248- Abstract
- 3-Hydroxypropionaldehyde (3HPA) is an important specialty chemical which can be produced from glycerol using resting cells of Lactobacillus reuteri. This biocatalytic route, however, suffers from substrate- and product-mediated loss of enzyme activity within 2 h of biotransformation. In order to overcome the inhibitory effects of 3HPA, complex formation with sodium bisulfite was investigated, optimized and applied for in situ capture of the aldehyde during biotransformation of glycerol in a fed-batch process. As a result, the activity of the cells was maintained for at least 18 h. The 3HPA produced per gram cell dry weight was increased 5.7 times compared to the batch production process, and 2.2 times compared to fed-batch process without... (More)
- 3-Hydroxypropionaldehyde (3HPA) is an important specialty chemical which can be produced from glycerol using resting cells of Lactobacillus reuteri. This biocatalytic route, however, suffers from substrate- and product-mediated loss of enzyme activity within 2 h of biotransformation. In order to overcome the inhibitory effects of 3HPA, complex formation with sodium bisulfite was investigated, optimized and applied for in situ capture of the aldehyde during biotransformation of glycerol in a fed-batch process. As a result, the activity of the cells was maintained for at least 18 h. The 3HPA produced per gram cell dry weight was increased 5.7 times compared to the batch production process, and 2.2 times compared to fed-batch process without in situ complex formation. This approach may have potential for production and in situ removal of 3HPA after further process development. Biotechnol. Bioeng. © 2012 Wiley Periodicals, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3218605
- author
- Sardari, Roya LU ; Dishisha, Tarek LU ; Pyo, Sang-Hyun LU and Hatti-Kaul, Rajni LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology and Bioengineering
- volume
- 110
- issue
- 4
- pages
- 1243 - 1248
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000315360100024
- pmid:23172314
- scopus:84874211159
- pmid:23172314
- ISSN
- 1097-0290
- DOI
- 10.1002/bit.24787
- language
- English
- LU publication?
- yes
- id
- 80b990b7-21dc-466c-9645-daadf4408b40 (old id 3218605)
- date added to LUP
- 2016-04-01 10:53:43
- date last changed
- 2024-01-07 03:54:06
@article{80b990b7-21dc-466c-9645-daadf4408b40, abstract = {{3-Hydroxypropionaldehyde (3HPA) is an important specialty chemical which can be produced from glycerol using resting cells of Lactobacillus reuteri. This biocatalytic route, however, suffers from substrate- and product-mediated loss of enzyme activity within 2 h of biotransformation. In order to overcome the inhibitory effects of 3HPA, complex formation with sodium bisulfite was investigated, optimized and applied for in situ capture of the aldehyde during biotransformation of glycerol in a fed-batch process. As a result, the activity of the cells was maintained for at least 18 h. The 3HPA produced per gram cell dry weight was increased 5.7 times compared to the batch production process, and 2.2 times compared to fed-batch process without in situ complex formation. This approach may have potential for production and in situ removal of 3HPA after further process development. Biotechnol. Bioeng. © 2012 Wiley Periodicals, Inc.}}, author = {{Sardari, Roya and Dishisha, Tarek and Pyo, Sang-Hyun and Hatti-Kaul, Rajni}}, issn = {{1097-0290}}, language = {{eng}}, number = {{4}}, pages = {{1243--1248}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Biotechnology and Bioengineering}}, title = {{Improved production of 3-Hydroxypropionaldehyde by complex formation with bisulfite during biotransformation of glycerol}}, url = {{http://dx.doi.org/10.1002/bit.24787}}, doi = {{10.1002/bit.24787}}, volume = {{110}}, year = {{2013}}, }