Activation of nuclear factor-kappa B pathway is responsible for tumor necrosis factor-a-induced up-regulation of endothelin B2 receptor expression in vascular smooth muscle cells in vitro
(2012) In Toxicology Letters 209(2). p.107-112- Abstract
- The endothelin B2 (ETB2) receptors are induced in vascular smooth muscle cells (VSMCs) in cardiovascular diseases. We tested if in vitro short-term exposure to the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) could up-regulate ETB2 receptors in rat mesenteric arteries, and if this effect is through activation of intracellular nuclear factor-kappa B (NF-kappa B) pathway. The mesenteric arteries were dissected from male Sprague-Dawley rats and the endothelium was removed. The arteries were co-incubated with TNF-alpha in serum-free Dulbecco's modified Eagle's medium. Real-time reverse transcription-PCR, Western blot and immunohistochemical staining were employed to assess the mRNA/protein expression of ETB2 receptors and... (More)
- The endothelin B2 (ETB2) receptors are induced in vascular smooth muscle cells (VSMCs) in cardiovascular diseases. We tested if in vitro short-term exposure to the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) could up-regulate ETB2 receptors in rat mesenteric arteries, and if this effect is through activation of intracellular nuclear factor-kappa B (NF-kappa B) pathway. The mesenteric arteries were dissected from male Sprague-Dawley rats and the endothelium was removed. The arteries were co-incubated with TNF-alpha in serum-free Dulbecco's modified Eagle's medium. Real-time reverse transcription-PCR, Western blot and immunohistochemical staining were employed to assess the mRNA/protein expression of ETB2 receptors and activation of NF-kappa B pathway. The results showed that, during organ culture. TNF-alpha concentration-dependently enhanced ET52 receptors expression at both mRNA and protein levels, paralleled with activation of NF-kappa B pathway in VSMC. The up-regulated ETB2 receptor expression and NF-kappa B activation could be effectively suppressed by general transcriptional inhibitor actinomycin D, or either of the selective I kappa B kinase inhibitors wedelolactone and IMD-0354. Conclusively, the activation of intracellular NF-kappa B pathway is responsible for the up-regulation of ETB2 receptors induced by short-term exposure to TNF-alpha. This could partly explain the toxic effects of TNF-alpha on VSMCs that account for cardiovascular diseases. (C) 2011 Elsevier Ireland Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2515569
- author
- Zhang, Wei ; Li, Xue-Jun ; Zeng, Xin ; Shen, Dong-Yan ; Liu, Chang-Qin ; Zhang, Hui-Jie ; Xu, Cang-Bao LU and Li, Xiao-Ying
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- factor-kappa B, Vascular smooth muscle cell, Nuclear, Tumor necrosis factor-alpha, Endothelin B2 receptor
- in
- Toxicology Letters
- volume
- 209
- issue
- 2
- pages
- 107 - 112
- publisher
- Elsevier
- external identifiers
-
- wos:000301277200001
- scopus:84862799831
- pmid:22207076
- ISSN
- 1879-3169
- DOI
- 10.1016/j.toxlet.2011.12.005
- language
- English
- LU publication?
- yes
- id
- 810f858f-a85c-42b4-8c62-3933946d8cbe (old id 2515569)
- date added to LUP
- 2016-04-01 12:58:37
- date last changed
- 2024-01-09 06:27:01
@article{810f858f-a85c-42b4-8c62-3933946d8cbe, abstract = {{The endothelin B2 (ETB2) receptors are induced in vascular smooth muscle cells (VSMCs) in cardiovascular diseases. We tested if in vitro short-term exposure to the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) could up-regulate ETB2 receptors in rat mesenteric arteries, and if this effect is through activation of intracellular nuclear factor-kappa B (NF-kappa B) pathway. The mesenteric arteries were dissected from male Sprague-Dawley rats and the endothelium was removed. The arteries were co-incubated with TNF-alpha in serum-free Dulbecco's modified Eagle's medium. Real-time reverse transcription-PCR, Western blot and immunohistochemical staining were employed to assess the mRNA/protein expression of ETB2 receptors and activation of NF-kappa B pathway. The results showed that, during organ culture. TNF-alpha concentration-dependently enhanced ET52 receptors expression at both mRNA and protein levels, paralleled with activation of NF-kappa B pathway in VSMC. The up-regulated ETB2 receptor expression and NF-kappa B activation could be effectively suppressed by general transcriptional inhibitor actinomycin D, or either of the selective I kappa B kinase inhibitors wedelolactone and IMD-0354. Conclusively, the activation of intracellular NF-kappa B pathway is responsible for the up-regulation of ETB2 receptors induced by short-term exposure to TNF-alpha. This could partly explain the toxic effects of TNF-alpha on VSMCs that account for cardiovascular diseases. (C) 2011 Elsevier Ireland Ltd. All rights reserved.}}, author = {{Zhang, Wei and Li, Xue-Jun and Zeng, Xin and Shen, Dong-Yan and Liu, Chang-Qin and Zhang, Hui-Jie and Xu, Cang-Bao and Li, Xiao-Ying}}, issn = {{1879-3169}}, keywords = {{factor-kappa B; Vascular smooth muscle cell; Nuclear; Tumor necrosis factor-alpha; Endothelin B2 receptor}}, language = {{eng}}, number = {{2}}, pages = {{107--112}}, publisher = {{Elsevier}}, series = {{Toxicology Letters}}, title = {{Activation of nuclear factor-kappa B pathway is responsible for tumor necrosis factor-a-induced up-regulation of endothelin B2 receptor expression in vascular smooth muscle cells in vitro}}, url = {{https://lup.lub.lu.se/search/files/3081909/2827519.pdf}}, doi = {{10.1016/j.toxlet.2011.12.005}}, volume = {{209}}, year = {{2012}}, }