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Cultivation technology development of Rhodothermus marinus DSM 16675

Ron, Emanuel Y C LU ; Sardari, Roya R R LU ; Anthony, Richard ; van Niel, Ed W J LU ; Hreggvidsson, Gudmundur O and Nordberg-Karlsson, Eva LU (2019) In Extremophiles : life under extreme conditions 23(6). p.735-745
Abstract

This work presents an evaluation of batch, fed-batch, and sequential batch cultivation techniques for production of R. marinus DSM 16675 and its exopolysaccharides (EPSs) and carotenoids in a bioreactor, using lysogeny broth (LB) and marine broth (MB), respectively, in both cases supplemented with 10 g/L maltose. Batch cultivation using LB supplemented with maltose (LBmalt) resulted in higher cell density (OD620 = 6.6) than use of MBmalt (OD620 = 1.7). Sequential batch cultivation increased the cell density threefold (OD620 = 20) in LBmalt and eightfold (OD620 = 14) in MBmalt. In both single and sequential batches, the production of carotenoids and EPSs using LBmalt was detected in the exponential phase and stationary phase,... (More)

This work presents an evaluation of batch, fed-batch, and sequential batch cultivation techniques for production of R. marinus DSM 16675 and its exopolysaccharides (EPSs) and carotenoids in a bioreactor, using lysogeny broth (LB) and marine broth (MB), respectively, in both cases supplemented with 10 g/L maltose. Batch cultivation using LB supplemented with maltose (LBmalt) resulted in higher cell density (OD620 = 6.6) than use of MBmalt (OD620 = 1.7). Sequential batch cultivation increased the cell density threefold (OD620 = 20) in LBmalt and eightfold (OD620 = 14) in MBmalt. In both single and sequential batches, the production of carotenoids and EPSs using LBmalt was detected in the exponential phase and stationary phase, respectively, while in MBmalt formation of both products was detectable in both the exponential and stationary phases of the culture. Heteropolymeric EPSs were produced with an overall volumetric productivity (QE) of 0.67 (mg/L h) in MBmalt and the polymer contained xylose. In LB, QE was lower (0.1 mg/L h) and xylose could not be detected in the composition of the produced EPSs. In conclusion, this study showed the importance of a process design and medium source for production of R. marinus DSM 16675 and its metabolites.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bioreactor, Carotenoid, Exopolysaccharide, Rhodothermus marinus, Sequential batch cultivation
in
Extremophiles : life under extreme conditions
volume
23
issue
6
pages
11 pages
publisher
Springer
external identifiers
  • scopus:85073611756
  • pmid:31522265
  • pmid:31522265
ISSN
1433-4909
DOI
10.1007/s00792-019-01129-0
language
English
LU publication?
yes
id
81176ea1-aa1d-4eca-a3e8-89df0e4678b9
date added to LUP
2019-09-26 08:56:42
date last changed
2020-10-20 03:48:31
@article{81176ea1-aa1d-4eca-a3e8-89df0e4678b9,
  abstract     = {<p>This work presents an evaluation of batch, fed-batch, and sequential batch cultivation techniques for production of R. marinus DSM 16675 and its exopolysaccharides (EPSs) and carotenoids in a bioreactor, using lysogeny broth (LB) and marine broth (MB), respectively, in both cases supplemented with 10 g/L maltose. Batch cultivation using LB supplemented with maltose (LBmalt) resulted in higher cell density (OD620 = 6.6) than use of MBmalt (OD620 = 1.7). Sequential batch cultivation increased the cell density threefold (OD620 = 20) in LBmalt and eightfold (OD620 = 14) in MBmalt. In both single and sequential batches, the production of carotenoids and EPSs using LBmalt was detected in the exponential phase and stationary phase, respectively, while in MBmalt formation of both products was detectable in both the exponential and stationary phases of the culture. Heteropolymeric EPSs were produced with an overall volumetric productivity (QE) of 0.67 (mg/L h) in MBmalt and the polymer contained xylose. In LB, QE was lower (0.1 mg/L h) and xylose could not be detected in the composition of the produced EPSs. In conclusion, this study showed the importance of a process design and medium source for production of R. marinus DSM 16675 and its metabolites.</p>},
  author       = {Ron, Emanuel Y C and Sardari, Roya R R and Anthony, Richard and van Niel, Ed W J and Hreggvidsson, Gudmundur O and Nordberg-Karlsson, Eva},
  issn         = {1433-4909},
  language     = {eng},
  month        = {11},
  number       = {6},
  pages        = {735--745},
  publisher    = {Springer},
  series       = {Extremophiles : life under extreme conditions},
  title        = {Cultivation technology development of Rhodothermus marinus DSM 16675},
  url          = {http://dx.doi.org/10.1007/s00792-019-01129-0},
  doi          = {10.1007/s00792-019-01129-0},
  volume       = {23},
  year         = {2019},
}