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Screening for plant lectins by latex agglutination tests

Kaul, Rajni LU ; Read, John and Mattiasson, Bo LU (1991) In Phytochemistry 30(12). p.4005-4009
Abstract
The latex agglutination test has been applied as a detection system for lectins, the method being especially useful in locations where the dependence on blood for hemagglutination tests could be minimised. The binding of various glycoproteins and sugars individually to the latex particles facilitated the agglutination with lectins having varying sugar specificities. The glycoproteins used were ovalbumin, horseradish peroxidase, porcine mucin and fetuin, while N-acetylglucosamine, N-acetylgalactosamine comprised the sugars used for binding to latex. The sensitivity of the latex agglutination tests was comparable with that of hemagglutination tests. Sugar binding specificity of the lectins could also be determined by inhibition of the... (More)
The latex agglutination test has been applied as a detection system for lectins, the method being especially useful in locations where the dependence on blood for hemagglutination tests could be minimised. The binding of various glycoproteins and sugars individually to the latex particles facilitated the agglutination with lectins having varying sugar specificities. The glycoproteins used were ovalbumin, horseradish peroxidase, porcine mucin and fetuin, while N-acetylglucosamine, N-acetylgalactosamine comprised the sugars used for binding to latex. The sensitivity of the latex agglutination tests was comparable with that of hemagglutination tests. Sugar binding specificity of the lectins could also be determined by inhibition of the agglutination in the presence of corresponding free sugars. The method proved to be useful in screening crude seed extracts for the presence of lectins. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Phytochemistry
volume
30
issue
12
pages
4005 - 4009
publisher
Elsevier
external identifiers
  • scopus:0011085561
ISSN
0031-9422
language
English
LU publication?
yes
id
8119240b-edd9-475f-80cb-d2b84590cb0e
date added to LUP
2019-06-29 18:34:51
date last changed
2020-07-09 17:25:51
@article{8119240b-edd9-475f-80cb-d2b84590cb0e,
  abstract     = {The latex agglutination test has been applied as a detection system for lectins, the method being especially useful in locations where the dependence on blood for hemagglutination tests could be minimised. The binding of various glycoproteins and sugars individually to the latex particles facilitated the agglutination with lectins having varying sugar specificities. The glycoproteins used were ovalbumin, horseradish peroxidase, porcine mucin and fetuin, while N-acetylglucosamine, N-acetylgalactosamine comprised the sugars used for binding to latex. The sensitivity of the latex agglutination tests was comparable with that of hemagglutination tests. Sugar binding specificity of the lectins could also be determined by inhibition of the agglutination in the presence of corresponding free sugars. The method proved to be useful in screening crude seed extracts for the presence of lectins.},
  author       = {Kaul, Rajni and Read, John and Mattiasson, Bo},
  issn         = {0031-9422},
  language     = {eng},
  number       = {12},
  pages        = {4005--4009},
  publisher    = {Elsevier},
  series       = {Phytochemistry},
  title        = {Screening for plant lectins by latex agglutination tests},
  volume       = {30},
  year         = {1991},
}