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Structural and biochemical characterization of two heme binding sites on α1-microglobulin using site directed mutagenesis and molecular simulation.

Rutardottir, Sigurbjörg LU ; Karnaukhova, Elena; Nantasenamat, Chanin; Songtawee, Napat; Prachayasittikul, Virapong; Rajabi, Mohsen; Wester Rosenlöf, Lena LU ; Alayash, Abdu I and Åkerström, Bo LU (2016) In Biochimica et Biophysica Acta - Proteins and Proteomics 1864(1). p.29-41
Abstract
α1-Microglobulin (A1M) is a reductase and radical scavenger involved in physiological protection against oxidative damage. These functions were previously shown to be dependent upon cysteinyl-, C34, and lysyl side-chains, K(92, 118,130). A1M binds heme and the crystal structure suggests that C34 and H123 participate in a heme binding site. We have investigated the involvement of these five residues in the interactions with heme.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochimica et Biophysica Acta - Proteins and Proteomics
volume
1864
issue
1
pages
29 - 41
publisher
Elsevier
external identifiers
  • pmid:26497278
  • scopus:84946606197
  • wos:000370464300004
ISSN
1570-9639
DOI
10.1016/j.bbapap.2015.10.002
language
English
LU publication?
yes
id
fdad36cc-943b-4ada-8339-bd3cbc61da06 (old id 8148501)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26497278?dopt=Abstract
date added to LUP
2015-11-03 19:50:37
date last changed
2017-06-25 03:55:44
@article{fdad36cc-943b-4ada-8339-bd3cbc61da06,
  abstract     = {α1-Microglobulin (A1M) is a reductase and radical scavenger involved in physiological protection against oxidative damage. These functions were previously shown to be dependent upon cysteinyl-, C34, and lysyl side-chains, K(92, 118,130). A1M binds heme and the crystal structure suggests that C34 and H123 participate in a heme binding site. We have investigated the involvement of these five residues in the interactions with heme.},
  author       = {Rutardottir, Sigurbjörg and Karnaukhova, Elena and Nantasenamat, Chanin and Songtawee, Napat and Prachayasittikul, Virapong and Rajabi, Mohsen and Wester Rosenlöf, Lena and Alayash, Abdu I and Åkerström, Bo},
  issn         = {1570-9639},
  language     = {eng},
  number       = {1},
  pages        = {29--41},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta - Proteins and Proteomics},
  title        = {Structural and biochemical characterization of two heme binding sites on α1-microglobulin using site directed mutagenesis and molecular simulation.},
  url          = {http://dx.doi.org/10.1016/j.bbapap.2015.10.002},
  volume       = {1864},
  year         = {2016},
}