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Three Redundant Synthetases Secure Redox-Active Pigment Production in the Basidiomycete Paxillus involutus.

Braesel, Jana; Götze, Sebastian; Shah, Firoz LU ; Heine, Daniel; Tauber, James; Hertweck, Christian; Tunlid, Anders LU ; Stallforth, Pierre and Hoffmeister, Dirk (2015) In Chemistry and Biology 22(10). p.1325-1334
Abstract
The symbiotic fungus Paxillus involutus serves a critical role in maintaining forest ecosystems, which are carbon sinks of global importance. P. involutus produces involutin and other 2,5-diarylcyclopentenone pigments that presumably assist in the oxidative degradation of lignocellulose via Fenton chemistry. Their precise biosynthetic pathways, however, remain obscure. Using a combination of biochemical, genetic, and transcriptomic analyses, in addition to stable-isotope labeling with synthetic precursors, we show that atromentin is the key intermediate. Atromentin is made by tridomain synthetases of high similarity: InvA1, InvA2, and InvA5. An inactive atromentin synthetase, InvA3, gained activity after a domain swap that replaced its... (More)
The symbiotic fungus Paxillus involutus serves a critical role in maintaining forest ecosystems, which are carbon sinks of global importance. P. involutus produces involutin and other 2,5-diarylcyclopentenone pigments that presumably assist in the oxidative degradation of lignocellulose via Fenton chemistry. Their precise biosynthetic pathways, however, remain obscure. Using a combination of biochemical, genetic, and transcriptomic analyses, in addition to stable-isotope labeling with synthetic precursors, we show that atromentin is the key intermediate. Atromentin is made by tridomain synthetases of high similarity: InvA1, InvA2, and InvA5. An inactive atromentin synthetase, InvA3, gained activity after a domain swap that replaced its native thioesterase domain with that of InvA5. The found degree of multiplex biosynthetic capacity is unprecedented with fungi, and highlights the great importance of the metabolite for the producer. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Chemistry and Biology
volume
22
issue
10
pages
1325 - 1334
publisher
Cell Press
external identifiers
  • pmid:26496685
  • wos:000366140900005
  • scopus:84950252285
ISSN
1879-1301
DOI
10.1016/j.chembiol.2015.08.016
project
MICCS - Molecular Interactions Controlling soil Carbon Sequestration
language
English
LU publication?
yes
id
49d345d6-5921-4f42-b6be-1d7119941e10 (old id 8148519)
date added to LUP
2015-11-17 11:05:13
date last changed
2017-08-13 03:25:14
@article{49d345d6-5921-4f42-b6be-1d7119941e10,
  abstract     = {The symbiotic fungus Paxillus involutus serves a critical role in maintaining forest ecosystems, which are carbon sinks of global importance. P. involutus produces involutin and other 2,5-diarylcyclopentenone pigments that presumably assist in the oxidative degradation of lignocellulose via Fenton chemistry. Their precise biosynthetic pathways, however, remain obscure. Using a combination of biochemical, genetic, and transcriptomic analyses, in addition to stable-isotope labeling with synthetic precursors, we show that atromentin is the key intermediate. Atromentin is made by tridomain synthetases of high similarity: InvA1, InvA2, and InvA5. An inactive atromentin synthetase, InvA3, gained activity after a domain swap that replaced its native thioesterase domain with that of InvA5. The found degree of multiplex biosynthetic capacity is unprecedented with fungi, and highlights the great importance of the metabolite for the producer.},
  author       = {Braesel, Jana and Götze, Sebastian and Shah, Firoz and Heine, Daniel and Tauber, James and Hertweck, Christian and Tunlid, Anders and Stallforth, Pierre and Hoffmeister, Dirk},
  issn         = {1879-1301},
  language     = {eng},
  number       = {10},
  pages        = {1325--1334},
  publisher    = {Cell Press},
  series       = {Chemistry and Biology},
  title        = {Three Redundant Synthetases Secure Redox-Active Pigment Production in the Basidiomycete Paxillus involutus.},
  url          = {http://dx.doi.org/10.1016/j.chembiol.2015.08.016},
  volume       = {22},
  year         = {2015},
}