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Surface nanostructures for fluorescence probing of supported lipid bilayers on reflective substrates.

Dabkowska, Aleksandra LU ; Piret, Gaelle LU ; Niman, Cassandra LU ; Lard, Mercy LU ; Linke, Heiner LU ; Nylander, Tommy LU and Prinz, Christelle LU (2015) In Nanoscale 7(43). p.18020-18024
Abstract
The fluorescence interference contrast (FLIC) effect prevents the use of fluorescence techniques to probe the continuity and fluidity of supported lipid bilayers on reflective materials due to a lack of detectable fluorescence. Here we show that adding nanostructures onto reflective surfaces to locally confer a certain distance between the deposited fluorophores and the reflecting surface enables fluorescence detection on the nanostuctures. The nanostructures consist of either deposited nanoparticles or epitaxial nanowires directly grown on the substrate and are designed such that they can support a lipid bilayer. This simple method increases the fluorescence signal sufficiently to enable bilayer fluorescence detection and to observe the... (More)
The fluorescence interference contrast (FLIC) effect prevents the use of fluorescence techniques to probe the continuity and fluidity of supported lipid bilayers on reflective materials due to a lack of detectable fluorescence. Here we show that adding nanostructures onto reflective surfaces to locally confer a certain distance between the deposited fluorophores and the reflecting surface enables fluorescence detection on the nanostuctures. The nanostructures consist of either deposited nanoparticles or epitaxial nanowires directly grown on the substrate and are designed such that they can support a lipid bilayer. This simple method increases the fluorescence signal sufficiently to enable bilayer fluorescence detection and to observe the recovery of fluorescence after photobleaching in order to assess lipid bilayer formation on any reflective surface. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nanoscale
volume
7
issue
43
pages
18020 - 18024
publisher
Royal Society of Chemistry
external identifiers
  • pmid:26482860
  • wos:000364048900005
  • scopus:84946108249
ISSN
2040-3372
DOI
10.1039/c5nr05427c
language
English
LU publication?
yes
id
5d937d2f-e859-4ea4-b093-839c5ae176d1 (old id 8148834)
date added to LUP
2015-11-16 21:44:08
date last changed
2017-01-01 03:10:43
@article{5d937d2f-e859-4ea4-b093-839c5ae176d1,
  abstract     = {The fluorescence interference contrast (FLIC) effect prevents the use of fluorescence techniques to probe the continuity and fluidity of supported lipid bilayers on reflective materials due to a lack of detectable fluorescence. Here we show that adding nanostructures onto reflective surfaces to locally confer a certain distance between the deposited fluorophores and the reflecting surface enables fluorescence detection on the nanostuctures. The nanostructures consist of either deposited nanoparticles or epitaxial nanowires directly grown on the substrate and are designed such that they can support a lipid bilayer. This simple method increases the fluorescence signal sufficiently to enable bilayer fluorescence detection and to observe the recovery of fluorescence after photobleaching in order to assess lipid bilayer formation on any reflective surface.},
  author       = {Dabkowska, Aleksandra and Piret, Gaelle and Niman, Cassandra and Lard, Mercy and Linke, Heiner and Nylander, Tommy and Prinz, Christelle},
  issn         = {2040-3372},
  language     = {eng},
  number       = {43},
  pages        = {18020--18024},
  publisher    = {Royal Society of Chemistry},
  series       = {Nanoscale},
  title        = {Surface nanostructures for fluorescence probing of supported lipid bilayers on reflective substrates.},
  url          = {http://dx.doi.org/10.1039/c5nr05427c},
  volume       = {7},
  year         = {2015},
}