Two-dimensional cell culturing on glass and plastic : the past, the present, and the future
(2022) p.21-35- Abstract
Two-dimensional (2D) cell culturing implies the cultivation of cells, isolated from an organism, to permit growth in 2D. Two-dimensional tissue culture surfaces are made of different kinds of glass and plastics with various degrees of hydrophilicity. The hydrophilicity is determined by the surface chemistry, which determines the adsorption of proteins required for cell attachment. Cellular physiology is highly affected by the surface chemistry due to the preadsorption of proteins. The relevance of 2D cell culturing must be considered in the context of how the cells grow in the organism. The use of 2D cell culturing for cancer cells is questionable, since a tumor is a disorganized three-dimensional (3D) structure. However, epithelial... (More)
Two-dimensional (2D) cell culturing implies the cultivation of cells, isolated from an organism, to permit growth in 2D. Two-dimensional tissue culture surfaces are made of different kinds of glass and plastics with various degrees of hydrophilicity. The hydrophilicity is determined by the surface chemistry, which determines the adsorption of proteins required for cell attachment. Cellular physiology is highly affected by the surface chemistry due to the preadsorption of proteins. The relevance of 2D cell culturing must be considered in the context of how the cells grow in the organism. The use of 2D cell culturing for cancer cells is questionable, since a tumor is a disorganized three-dimensional (3D) structure. However, epithelial cells grow in 2D although on a highly folded 2D basement membrane, which is supported by a 3D environment. To increase reproducibility of all cell culturing, standardization of surfaces and medium composition is required.
(Less)
- author
- Malakpour-Permlid, Atena LU and Oredsson, Stina LU
- organization
- publishing date
- 2022-01
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- cancer cells, cell culture medium, epithelial cells, glass surface, hydrophilicity, plastic surface, protein adsorption, reproducibility, surface chemistry, Two-dimensional
- host publication
- 3D Lung Models for Regenerating Lung Tissue
- pages
- 15 pages
- publisher
- ScienceDirect, Elsevier
- external identifiers
-
- scopus:85142859484
- ISBN
- 9780323908719
- 9780323908726
- DOI
- 10.1016/B978-0-323-90871-9.00008-5
- language
- English
- LU publication?
- yes
- id
- 815e3320-e232-4ac2-b7b3-48743ce45292
- date added to LUP
- 2022-12-27 11:54:53
- date last changed
- 2024-04-18 16:59:40
@inbook{815e3320-e232-4ac2-b7b3-48743ce45292,
abstract = {{<p>Two-dimensional (2D) cell culturing implies the cultivation of cells, isolated from an organism, to permit growth in 2D. Two-dimensional tissue culture surfaces are made of different kinds of glass and plastics with various degrees of hydrophilicity. The hydrophilicity is determined by the surface chemistry, which determines the adsorption of proteins required for cell attachment. Cellular physiology is highly affected by the surface chemistry due to the preadsorption of proteins. The relevance of 2D cell culturing must be considered in the context of how the cells grow in the organism. The use of 2D cell culturing for cancer cells is questionable, since a tumor is a disorganized three-dimensional (3D) structure. However, epithelial cells grow in 2D although on a highly folded 2D basement membrane, which is supported by a 3D environment. To increase reproducibility of all cell culturing, standardization of surfaces and medium composition is required.</p>}},
author = {{Malakpour-Permlid, Atena and Oredsson, Stina}},
booktitle = {{3D Lung Models for Regenerating Lung Tissue}},
isbn = {{9780323908719}},
keywords = {{cancer cells; cell culture medium; epithelial cells; glass surface; hydrophilicity; plastic surface; protein adsorption; reproducibility; surface chemistry; Two-dimensional}},
language = {{eng}},
pages = {{21--35}},
publisher = {{ScienceDirect, Elsevier}},
title = {{Two-dimensional cell culturing on glass and plastic : the past, the present, and the future}},
url = {{http://dx.doi.org/10.1016/B978-0-323-90871-9.00008-5}},
doi = {{10.1016/B978-0-323-90871-9.00008-5}},
year = {{2022}},
}