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Analysis of anti-C1q autoantibodies by western blot

Verlemyr, Anci ; Truedsson, Lennart LU and Skattum, Lillemor LU (2019) In Methods in Molecular Biology 1901. p.183-189
Abstract

Anti-C1q autoantibodies may be found in many conditions, most commonly in systemic lupus erythematosus (SLE) and hypocomplementemic urticarial vasculitis syndrome (HUVS), and are diagnostic markers as well as disease activity markers in lupus nephritis. Sera from patients with SLE and HUVS show partly distinct autoantibody reactivities to separated protein chains B and C of the first component of complement, C1q. These different binding specificities can be detected by Western blot analysis of the autoantibodies under reducing conditions. Results may help clinicians to differentiate between SLE and HUVS.

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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Anti-C1q autoantibodies, C1q protein chains B and C, Hypocomplementemic urticarial vasculitis syndrome, Systemic lupus erythematosus, Western blot
host publication
Methods in Molecular Biology
series title
Methods in Molecular Biology
volume
1901
pages
7 pages
publisher
Humana Press
external identifiers
  • scopus:85058611402
  • pmid:30539577
ISSN
1064-3745
DOI
10.1007/978-1-4939-8949-2_14
language
English
LU publication?
yes
id
81b104d2-9d64-4428-a7e3-789f73fb3e4f
date added to LUP
2019-01-02 14:39:50
date last changed
2019-11-25 09:26:25
@inbook{81b104d2-9d64-4428-a7e3-789f73fb3e4f,
  abstract     = {<p>Anti-C1q autoantibodies may be found in many conditions, most commonly in systemic lupus erythematosus (SLE) and hypocomplementemic urticarial vasculitis syndrome (HUVS), and are diagnostic markers as well as disease activity markers in lupus nephritis. Sera from patients with SLE and HUVS show partly distinct autoantibody reactivities to separated protein chains B and C of the first component of complement, C1q. These different binding specificities can be detected by Western blot analysis of the autoantibodies under reducing conditions. Results may help clinicians to differentiate between SLE and HUVS.</p>},
  author       = {Verlemyr, Anci and Truedsson, Lennart and Skattum, Lillemor},
  booktitle    = {Methods in Molecular Biology},
  issn         = {1064-3745},
  language     = {eng},
  pages        = {183--189},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Analysis of anti-C1q autoantibodies by western blot},
  url          = {http://dx.doi.org/10.1007/978-1-4939-8949-2_14},
  doi          = {10.1007/978-1-4939-8949-2_14},
  volume       = {1901},
  year         = {2019},
}