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Sex pheromone biosynthesis in the sugarcane borer Diatraea saccharalis : paving the way for biotechnological production

Dam, Marie Inger LU ; Ding, Bao Jian LU ; Svensson, Glenn P. LU ; Wang, Hong Lei LU ; Melo, Douglas J. LU ; Lassance, Jean Marc LU ; Zarbin, Paulo H.G. and Löfstedt, Christer LU (2023) In Pest Management Science
Abstract

BACKGROUND: The sugarcane borer Diatraea saccharalis (Lepidoptera) is a key pest on sugarcane and other grasses in the Americas. Biological control as well as insecticide treatments are used for pest management, but economic losses are still significant. The use of female sex pheromones for mating disruption or mass trapping in pest management could be established for this species, provided that economical production of pheromone is available. RESULTS: Combining in vivo labelling studies, differential expression analysis of transcriptome data and functional characterisation of insect genes in a yeast expression system, we reveal the biosynthetic pathway and identify the desaturase and reductase enzymes involved in the biosynthesis of... (More)

BACKGROUND: The sugarcane borer Diatraea saccharalis (Lepidoptera) is a key pest on sugarcane and other grasses in the Americas. Biological control as well as insecticide treatments are used for pest management, but economic losses are still significant. The use of female sex pheromones for mating disruption or mass trapping in pest management could be established for this species, provided that economical production of pheromone is available. RESULTS: Combining in vivo labelling studies, differential expression analysis of transcriptome data and functional characterisation of insect genes in a yeast expression system, we reveal the biosynthetic pathway and identify the desaturase and reductase enzymes involved in the biosynthesis of the main pheromone component (9Z,11E)-hexadecadienal, and minor components hexadecanal, (9Z)-hexadecenal and (11Z)-hexadecenal. We next demonstrate heterologous production of the corresponding alcohols of the pheromone components, by expressing multiple steps of the biosynthetic pathway in yeast. CONCLUSION: Elucidation of the genetic basis of sex pheromone biosynthesis in D. saccharalis, and heterologous expression in yeast, paves the way for biotechnological production of the pheromone compounds needed for pheromone-based pest management of this species.

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author
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organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
desaturase, functional characterisation, heterologous expression, integrated pest management, lepidoptera, transcriptome
in
Pest Management Science
pages
12 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:37830147
  • scopus:85176113497
ISSN
1526-498X
DOI
10.1002/ps.7830
language
English
LU publication?
yes
id
81f1d657-03be-4456-af97-ba1b2f05fc71
date added to LUP
2023-12-05 16:20:45
date last changed
2024-04-18 12:23:45
@article{81f1d657-03be-4456-af97-ba1b2f05fc71,
  abstract     = {{<p>BACKGROUND: The sugarcane borer Diatraea saccharalis (Lepidoptera) is a key pest on sugarcane and other grasses in the Americas. Biological control as well as insecticide treatments are used for pest management, but economic losses are still significant. The use of female sex pheromones for mating disruption or mass trapping in pest management could be established for this species, provided that economical production of pheromone is available. RESULTS: Combining in vivo labelling studies, differential expression analysis of transcriptome data and functional characterisation of insect genes in a yeast expression system, we reveal the biosynthetic pathway and identify the desaturase and reductase enzymes involved in the biosynthesis of the main pheromone component (9Z,11E)-hexadecadienal, and minor components hexadecanal, (9Z)-hexadecenal and (11Z)-hexadecenal. We next demonstrate heterologous production of the corresponding alcohols of the pheromone components, by expressing multiple steps of the biosynthetic pathway in yeast. CONCLUSION: Elucidation of the genetic basis of sex pheromone biosynthesis in D. saccharalis, and heterologous expression in yeast, paves the way for biotechnological production of the pheromone compounds needed for pheromone-based pest management of this species.</p>}},
  author       = {{Dam, Marie Inger and Ding, Bao Jian and Svensson, Glenn P. and Wang, Hong Lei and Melo, Douglas J. and Lassance, Jean Marc and Zarbin, Paulo H.G. and Löfstedt, Christer}},
  issn         = {{1526-498X}},
  keywords     = {{desaturase; functional characterisation; heterologous expression; integrated pest management; lepidoptera; transcriptome}},
  language     = {{eng}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Pest Management Science}},
  title        = {{Sex pheromone biosynthesis in the sugarcane borer Diatraea saccharalis : paving the way for biotechnological production}},
  url          = {{http://dx.doi.org/10.1002/ps.7830}},
  doi          = {{10.1002/ps.7830}},
  year         = {{2023}},
}