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Emulsifying triglycerides with dairy phospholipids instead of soy lecithin modulates gut lipase activity

Mathiassen, Jakob Hovalt; Nejrup, Rikke Guldhammer; Frokiaer, Hanne; Nilsson, Åke LU ; Ohlsson, Lena LU and Hellgren, Lars I. (2015) In European Journal of Lipid Science and Technology 117(10). p.1522-1539
Abstract
A high energy supply and appropriate amount of polyunsaturated fatty acids is crucial for proper growth and development in neonates. The capacity for fatty acid absorption in newborns is however hampered due to an undeveloped pancreatic function combined with a low bile output. This seems in particular to limit fatty acid absorption in babies given infant formulas. Since interaction between the lipid droplet and the gastric and duodenal lipases occur through the hydrophobic/hydrophilic interface, the composition of the emulsifier may be crucial for efficient hydrolysis. We therefore determined hydrolytic rate of gastric lipase and pancreatic lipase, on their own or pancreatic lipase after gastric lipase on TAG droplets of similar size... (More)
A high energy supply and appropriate amount of polyunsaturated fatty acids is crucial for proper growth and development in neonates. The capacity for fatty acid absorption in newborns is however hampered due to an undeveloped pancreatic function combined with a low bile output. This seems in particular to limit fatty acid absorption in babies given infant formulas. Since interaction between the lipid droplet and the gastric and duodenal lipases occur through the hydrophobic/hydrophilic interface, the composition of the emulsifier may be crucial for efficient hydrolysis. We therefore determined hydrolytic rate of gastric lipase and pancreatic lipase, on their own or pancreatic lipase after gastric lipase on TAG droplets of similar size emulsified in either soy lecithin (SL) or in bovine milk phospholipids (MPL), more similar to human milk globule membrane lipids than soy lecithin. Gastric lipase activity was substantially higher on MPL-emulsified particles, while SL emulsification caused a higher rate of pancreatic lipase hydrolysis, on particles that had not been pre-treated with gastric lipase. MPL emulsification did however cause higher pancreatic lipase activity, when the particles had been pre-treated with gastric lipase. The attenuating effect of MPL-emulsification on pancreas lipase activity was validated in vivo in mice. Practical application: Our results show that the activity of gastric lipase, a key enzyme in lipid absorption in neonates, is highly dependent on the phospholipid composition of the surface layer on the emulsion droplets. We furthermore show that increasing gastric lipase activity by modifying the phospholipid composition on the droplet surface, also enhance the subsequent activity of the pancreatic lipase acting in the duodenum. This implies that it is possible to strategically design the surface layer of lipid droplets in infant formulas to maximize gastric lipase activity, and that this could improve total fatty acid absorption in formula-fed neonates. This is of particular importance in the development of formula aimed at pre-mature babies, but is also highly relevant for formulas for term-born infants. Emulsifying corn oil in milk phospholipids rather than soy lecithin causes a substantial increase in gastric lipase activity (upper). The increased gastric lipase activity on the emulsion particles also causes a threefold increase in pancreatic lipase activity, when done in sequence of gastric lipase hydrolysis (lower). MPL=milk phospholipids, SL=soy lecithin, w/o GL=without gastric lipase pre-treatment, w GL=with gastric lipase pre-treatment. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Emulsifier, Gastric lipase, Human milk, Infant formula, Lipid hydrolysis, rate, Milk-fat globule membrane, Pancreatic lipase, Phospholipids
in
European Journal of Lipid Science and Technology
volume
117
issue
10
pages
1522 - 1539
publisher
John Wiley & Sons
external identifiers
  • wos:000362964000006
  • scopus:84944155587
ISSN
1438-7697
DOI
10.1002/ejlt.201400505
language
English
LU publication?
yes
id
bfdbfe71-e7d6-467c-b6ff-af445657ff23 (old id 8205930)
date added to LUP
2015-12-01 07:06:57
date last changed
2017-09-24 03:22:18
@article{bfdbfe71-e7d6-467c-b6ff-af445657ff23,
  abstract     = {A high energy supply and appropriate amount of polyunsaturated fatty acids is crucial for proper growth and development in neonates. The capacity for fatty acid absorption in newborns is however hampered due to an undeveloped pancreatic function combined with a low bile output. This seems in particular to limit fatty acid absorption in babies given infant formulas. Since interaction between the lipid droplet and the gastric and duodenal lipases occur through the hydrophobic/hydrophilic interface, the composition of the emulsifier may be crucial for efficient hydrolysis. We therefore determined hydrolytic rate of gastric lipase and pancreatic lipase, on their own or pancreatic lipase after gastric lipase on TAG droplets of similar size emulsified in either soy lecithin (SL) or in bovine milk phospholipids (MPL), more similar to human milk globule membrane lipids than soy lecithin. Gastric lipase activity was substantially higher on MPL-emulsified particles, while SL emulsification caused a higher rate of pancreatic lipase hydrolysis, on particles that had not been pre-treated with gastric lipase. MPL emulsification did however cause higher pancreatic lipase activity, when the particles had been pre-treated with gastric lipase. The attenuating effect of MPL-emulsification on pancreas lipase activity was validated in vivo in mice. Practical application: Our results show that the activity of gastric lipase, a key enzyme in lipid absorption in neonates, is highly dependent on the phospholipid composition of the surface layer on the emulsion droplets. We furthermore show that increasing gastric lipase activity by modifying the phospholipid composition on the droplet surface, also enhance the subsequent activity of the pancreatic lipase acting in the duodenum. This implies that it is possible to strategically design the surface layer of lipid droplets in infant formulas to maximize gastric lipase activity, and that this could improve total fatty acid absorption in formula-fed neonates. This is of particular importance in the development of formula aimed at pre-mature babies, but is also highly relevant for formulas for term-born infants. Emulsifying corn oil in milk phospholipids rather than soy lecithin causes a substantial increase in gastric lipase activity (upper). The increased gastric lipase activity on the emulsion particles also causes a threefold increase in pancreatic lipase activity, when done in sequence of gastric lipase hydrolysis (lower). MPL=milk phospholipids, SL=soy lecithin, w/o GL=without gastric lipase pre-treatment, w GL=with gastric lipase pre-treatment.},
  author       = {Mathiassen, Jakob Hovalt and Nejrup, Rikke Guldhammer and Frokiaer, Hanne and Nilsson, Åke and Ohlsson, Lena and Hellgren, Lars I.},
  issn         = {1438-7697},
  keyword      = {Emulsifier,Gastric lipase,Human milk,Infant formula,Lipid hydrolysis,rate,Milk-fat globule membrane,Pancreatic lipase,Phospholipids},
  language     = {eng},
  number       = {10},
  pages        = {1522--1539},
  publisher    = {John Wiley & Sons},
  series       = {European Journal of Lipid Science and Technology},
  title        = {Emulsifying triglycerides with dairy phospholipids instead of soy lecithin modulates gut lipase activity},
  url          = {http://dx.doi.org/10.1002/ejlt.201400505},
  volume       = {117},
  year         = {2015},
}