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The association between plasma 25-hydroxyvitamin D and subgroups in age-related macular degeneration : a cross-sectional study

Singh, Amardeep LU ; Falk, Mads Krüger ; Subhi, Yousif and Sørensen, Torben Lykke (2013) In PLoS ONE 8(7). p.1-9
Abstract

OBJECTIVES: To evaluate potential differences in plasma 25-hydroxyvitamin in subtypes of age-related macular degeneration (AMD), and in patients in Clinical Age-Related Maculopathy Staging (CARMS) group 5 with or without subretinal fibrosis.

METHODS: This single-center cross-sectional study included 178 participants during a period of 20 months. Ninety-five patients belonged to CARMS 5; twelve belonged to CARMS 4; twenty-two belonged to CARMS 2 or 3; and 49 individuals did not have AMD (CARMS 1). Following a structured interview, a detailed bilateral retinal examination was performed and participants were allocated to their respective subgroups in accordance with the Clinical Age-Related Maculopathy Staging system. Plasma... (More)

OBJECTIVES: To evaluate potential differences in plasma 25-hydroxyvitamin in subtypes of age-related macular degeneration (AMD), and in patients in Clinical Age-Related Maculopathy Staging (CARMS) group 5 with or without subretinal fibrosis.

METHODS: This single-center cross-sectional study included 178 participants during a period of 20 months. Ninety-five patients belonged to CARMS 5; twelve belonged to CARMS 4; twenty-two belonged to CARMS 2 or 3; and 49 individuals did not have AMD (CARMS 1). Following a structured interview, a detailed bilateral retinal examination was performed and participants were allocated to their respective subgroups in accordance with the Clinical Age-Related Maculopathy Staging system. Plasma 25-hydroxyvitamin D2 and D3 were analyzed using liquid chromatography-tandem mass spectrometry. Genomic DNA was extracted from leukocytes and genotyped for single nucleotide polymorphisms (SNPs) in the vitamin D metabolism. Differences in plasma 25-hydroxyvitamin D were determined in the subgroups as well as between patients in CARMS 5 with or without subretinal fibrosis.

RESULTS: Plasma 25-hydroxyvitamin D was comparable in patients across CARMS groups 1 to 5 (p = 0.83). In CARMS 5, the presence of subretinal fibrosis was associated with significantly lower concentrations of 25-hydroxyvitamin D as compared to the absence of subretinal fibrosis (47.2 versus 75.6 nmol/L, p<0.001). Patients in CARMS 5 with subretinal fibrosis were more likely to have insufficient levels of 25-hydroxyvitamin D compared to patients without subretinal fibrosis (p = 0.006). No association was found between the SNPs rs10877012, rs2228570, rs4588, or rs7041 and AMD subgroups or plasma 25-hydroxyvitamin levels.

CONCLUSIONS: This study suggests that the presence of subretinal fibrosis in patients belonging to CARMS 5 may be associated with a poor vitamin D status. Our observations warrant further investigation into the role of vitamin D in the development of subretinal fibrosis.

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author
; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aged, Aged, 80 and over, Cross-Sectional Studies, Female, Fibrosis, Humans, Macular Degeneration/blood, Male, Polymorphism, Single Nucleotide, Retina/pathology, Risk Factors, Seasons, Vitamin D/analogs & derivatives
in
PLoS ONE
volume
8
issue
7
article number
e70948
pages
1 - 9
publisher
Public Library of Science (PLoS)
external identifiers
  • pmid:23923033
  • scopus:84880763661
ISSN
1932-6203
DOI
10.1371/journal.pone.0070948
language
English
LU publication?
no
id
822b9417-25f1-4f77-a882-fb3174d8a6c8
date added to LUP
2019-05-21 10:53:42
date last changed
2024-05-28 11:32:11
@article{822b9417-25f1-4f77-a882-fb3174d8a6c8,
  abstract     = {{<p>OBJECTIVES: To evaluate potential differences in plasma 25-hydroxyvitamin in subtypes of age-related macular degeneration (AMD), and in patients in Clinical Age-Related Maculopathy Staging (CARMS) group 5 with or without subretinal fibrosis.</p><p>METHODS: This single-center cross-sectional study included 178 participants during a period of 20 months. Ninety-five patients belonged to CARMS 5; twelve belonged to CARMS 4; twenty-two belonged to CARMS 2 or 3; and 49 individuals did not have AMD (CARMS 1). Following a structured interview, a detailed bilateral retinal examination was performed and participants were allocated to their respective subgroups in accordance with the Clinical Age-Related Maculopathy Staging system. Plasma 25-hydroxyvitamin D2 and D3 were analyzed using liquid chromatography-tandem mass spectrometry. Genomic DNA was extracted from leukocytes and genotyped for single nucleotide polymorphisms (SNPs) in the vitamin D metabolism. Differences in plasma 25-hydroxyvitamin D were determined in the subgroups as well as between patients in CARMS 5 with or without subretinal fibrosis.</p><p>RESULTS: Plasma 25-hydroxyvitamin D was comparable in patients across CARMS groups 1 to 5 (p = 0.83). In CARMS 5, the presence of subretinal fibrosis was associated with significantly lower concentrations of 25-hydroxyvitamin D as compared to the absence of subretinal fibrosis (47.2 versus 75.6 nmol/L, p&lt;0.001). Patients in CARMS 5 with subretinal fibrosis were more likely to have insufficient levels of 25-hydroxyvitamin D compared to patients without subretinal fibrosis (p = 0.006). No association was found between the SNPs rs10877012, rs2228570, rs4588, or rs7041 and AMD subgroups or plasma 25-hydroxyvitamin levels.</p><p>CONCLUSIONS: This study suggests that the presence of subretinal fibrosis in patients belonging to CARMS 5 may be associated with a poor vitamin D status. Our observations warrant further investigation into the role of vitamin D in the development of subretinal fibrosis.</p>}},
  author       = {{Singh, Amardeep and Falk, Mads Krüger and Subhi, Yousif and Sørensen, Torben Lykke}},
  issn         = {{1932-6203}},
  keywords     = {{Aged; Aged, 80 and over; Cross-Sectional Studies; Female; Fibrosis; Humans; Macular Degeneration/blood; Male; Polymorphism, Single Nucleotide; Retina/pathology; Risk Factors; Seasons; Vitamin D/analogs & derivatives}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{1--9}},
  publisher    = {{Public Library of Science (PLoS)}},
  series       = {{PLoS ONE}},
  title        = {{The association between plasma 25-hydroxyvitamin D and subgroups in age-related macular degeneration : a cross-sectional study}},
  url          = {{http://dx.doi.org/10.1371/journal.pone.0070948}},
  doi          = {{10.1371/journal.pone.0070948}},
  volume       = {{8}},
  year         = {{2013}},
}