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Acylated monogalactosyl diacylglycerol: Prevalence in the plant kingdom and identification of an enzyme catalyzing galactolipid head group acylation in Arabidopsis thaliana.

Nilsson, Anders K; Johansson, Oskar N; Fahlberg, Per; Kommuri, Murali; Töpel, Mats; Bodin, Lovisa J; Sikora, Per; Modarres, Masoomeh; Ekengren, Sophia and Nguyen, Chi Tam, et al. (2015) In Plant Journal 84(6). p.1152-1166
Abstract
The lipid phase of the thylakoid membrane is mainly composed of the galalctolipids mono- and digalactosyl diacylglycerol (MGDG and DGDG, respectively). It has been known since the late sixties that MGDG can be acylated with a third fatty acid to the galactose head group (acyl-MGDG) in plant leaf homogenates. In certain brassicaceous plants like Arabidopsis thaliana, the acyl-MGDG frequently incorporates oxidized fatty acids in the form of the jasmonic acid precursor 12-oxo-phytodienoic acid (OPDA). In the present study we further investigated the distribution of acylated and OPDA-containing galactolipids in the plant kingdom. While acyl-MGDG was found to be ubiquitous in green tissue of plants ranging from non-vascular plants to... (More)
The lipid phase of the thylakoid membrane is mainly composed of the galalctolipids mono- and digalactosyl diacylglycerol (MGDG and DGDG, respectively). It has been known since the late sixties that MGDG can be acylated with a third fatty acid to the galactose head group (acyl-MGDG) in plant leaf homogenates. In certain brassicaceous plants like Arabidopsis thaliana, the acyl-MGDG frequently incorporates oxidized fatty acids in the form of the jasmonic acid precursor 12-oxo-phytodienoic acid (OPDA). In the present study we further investigated the distribution of acylated and OPDA-containing galactolipids in the plant kingdom. While acyl-MGDG was found to be ubiquitous in green tissue of plants ranging from non-vascular plants to angiosperms, OPDA-containing galactolipids were only present in plants from a few genera A candidate protein responsible for the acyl transfer was identified in Avena sativa (oat) leaf tissue using biochemical fractionation and proteomcs. Knockout of the orthologous gene in A. thaliana resulted in an almost total elimination of the ability to form both non-oxidized and OPDA-containing acyl-MGDG. In addition, heterologous expression of the A. thaliana gene in E. coli demonstrated that the protein catalyzed acylation of MGDG. We thus demonstrate that a phylogenetically conserved enzyme is responsible for the accumulation of acyl-MGDG in A. thaliana. The activity of this enzyme in vivo is strongly enhanced by freezing damage and the hypersensitive response. This article is protected by copyright. All rights reserved. (Less)
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publication status
published
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Plant Journal
volume
84
issue
6
pages
1152 - 1166
publisher
Wiley-Blackwell
external identifiers
  • pmid:26566971
  • wos:000368268700009
  • scopus:84961353304
ISSN
1365-313X
DOI
10.1111/tpj.13072
language
English
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yes
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797c78c3-a8a1-4d01-9b39-03a0ba67342b (old id 8235549)
date added to LUP
2015-12-16 17:33:53
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2017-10-01 03:05:07
@article{797c78c3-a8a1-4d01-9b39-03a0ba67342b,
  abstract     = {The lipid phase of the thylakoid membrane is mainly composed of the galalctolipids mono- and digalactosyl diacylglycerol (MGDG and DGDG, respectively). It has been known since the late sixties that MGDG can be acylated with a third fatty acid to the galactose head group (acyl-MGDG) in plant leaf homogenates. In certain brassicaceous plants like Arabidopsis thaliana, the acyl-MGDG frequently incorporates oxidized fatty acids in the form of the jasmonic acid precursor 12-oxo-phytodienoic acid (OPDA). In the present study we further investigated the distribution of acylated and OPDA-containing galactolipids in the plant kingdom. While acyl-MGDG was found to be ubiquitous in green tissue of plants ranging from non-vascular plants to angiosperms, OPDA-containing galactolipids were only present in plants from a few genera A candidate protein responsible for the acyl transfer was identified in Avena sativa (oat) leaf tissue using biochemical fractionation and proteomcs. Knockout of the orthologous gene in A. thaliana resulted in an almost total elimination of the ability to form both non-oxidized and OPDA-containing acyl-MGDG. In addition, heterologous expression of the A. thaliana gene in E. coli demonstrated that the protein catalyzed acylation of MGDG. We thus demonstrate that a phylogenetically conserved enzyme is responsible for the accumulation of acyl-MGDG in A. thaliana. The activity of this enzyme in vivo is strongly enhanced by freezing damage and the hypersensitive response. This article is protected by copyright. All rights reserved.},
  author       = {Nilsson, Anders K and Johansson, Oskar N and Fahlberg, Per and Kommuri, Murali and Töpel, Mats and Bodin, Lovisa J and Sikora, Per and Modarres, Masoomeh and Ekengren, Sophia and Nguyen, Chi Tam and Farmer, Edward E and Olsson, Olof and Ellerström, Mats and Andersson, Mats X},
  issn         = {1365-313X},
  language     = {eng},
  number       = {6},
  pages        = {1152--1166},
  publisher    = {Wiley-Blackwell},
  series       = {Plant Journal},
  title        = {Acylated monogalactosyl diacylglycerol: Prevalence in the plant kingdom and identification of an enzyme catalyzing galactolipid head group acylation in Arabidopsis thaliana.},
  url          = {http://dx.doi.org/10.1111/tpj.13072},
  volume       = {84},
  year         = {2015},
}