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Impacts of inoculum pre-treatments on enzyme activity and biochemical methane potential.

Wang, Bing LU ; Strömberg, Sten LU ; Ivo Achu, Nges LU ; Nistor, Mihaela LU and Liu, Jing LU (2015) In Journal of Bioscience and Bioengineering
Abstract
Biochemical methane potential (BMP) tests were carried out to investigate the influence of inoculum pre-treatments (filtration and pre-incubation) on methane production from cellulose and wheat straw. First-order model and Monod model were used to evaluate the kinetic constants of the BMP assays. The results demonstrated that fresh inoculum was the best option to perform BMP tests. This was evidenced by highest enzyme activity (0.11 U/mL) and highest methane yields for cellulose (356 NmL CH4/gVS) as well as wheat straw (261 NmL CH4/gVS). Besides, high biodegradability (85.8% for cellulose and 61.3% for wheat straw) was also obtained when the fresh inoculum was used. Moreover, a kinetic evaluation showed that inoculum pre-incubation at 37°C... (More)
Biochemical methane potential (BMP) tests were carried out to investigate the influence of inoculum pre-treatments (filtration and pre-incubation) on methane production from cellulose and wheat straw. First-order model and Monod model were used to evaluate the kinetic constants of the BMP assays. The results demonstrated that fresh inoculum was the best option to perform BMP tests. This was evidenced by highest enzyme activity (0.11 U/mL) and highest methane yields for cellulose (356 NmL CH4/gVS) as well as wheat straw (261 NmL CH4/gVS). Besides, high biodegradability (85.8% for cellulose and 61.3% for wheat straw) was also obtained when the fresh inoculum was used. Moreover, a kinetic evaluation showed that inoculum pre-incubation at 37°C or storage at 4°C introduced a lag-time whereas the effects on hydrolysis rate were less consequent. In summary, pre-treatments affected the enzyme activity of the inoculum, and further on, significantly influenced the methane production and the degradation kinetics of the investigated substrates. It is recommended that filtration of inoculum should be avoided unless in case too large particles therein. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Journal of Bioscience and Bioengineering
publisher
Elsevier
external identifiers
  • pmid:26526543
  • scopus:84961253893
ISSN
1347-4421
DOI
10.1016/j.jbiosc.2015.10.004
language
English
LU publication?
yes
id
d0b20681-a2bd-4bf5-91d7-6311c265f732 (old id 8243543)
date added to LUP
2015-12-08 10:13:57
date last changed
2017-01-29 03:02:13
@article{d0b20681-a2bd-4bf5-91d7-6311c265f732,
  abstract     = {Biochemical methane potential (BMP) tests were carried out to investigate the influence of inoculum pre-treatments (filtration and pre-incubation) on methane production from cellulose and wheat straw. First-order model and Monod model were used to evaluate the kinetic constants of the BMP assays. The results demonstrated that fresh inoculum was the best option to perform BMP tests. This was evidenced by highest enzyme activity (0.11 U/mL) and highest methane yields for cellulose (356 NmL CH4/gVS) as well as wheat straw (261 NmL CH4/gVS). Besides, high biodegradability (85.8% for cellulose and 61.3% for wheat straw) was also obtained when the fresh inoculum was used. Moreover, a kinetic evaluation showed that inoculum pre-incubation at 37°C or storage at 4°C introduced a lag-time whereas the effects on hydrolysis rate were less consequent. In summary, pre-treatments affected the enzyme activity of the inoculum, and further on, significantly influenced the methane production and the degradation kinetics of the investigated substrates. It is recommended that filtration of inoculum should be avoided unless in case too large particles therein.},
  author       = {Wang, Bing and Strömberg, Sten and Ivo Achu, Nges and Nistor, Mihaela and Liu, Jing},
  issn         = {1347-4421},
  language     = {eng},
  month        = {10},
  publisher    = {Elsevier},
  series       = {Journal of Bioscience and Bioengineering},
  title        = {Impacts of inoculum pre-treatments on enzyme activity and biochemical methane potential.},
  url          = {http://dx.doi.org/10.1016/j.jbiosc.2015.10.004},
  year         = {2015},
}