Impacts of inoculum pre-treatments on enzyme activity and biochemical methane potential.
(2015) In Journal of Bioscience and Bioengineering- Abstract
- Biochemical methane potential (BMP) tests were carried out to investigate the influence of inoculum pre-treatments (filtration and pre-incubation) on methane production from cellulose and wheat straw. First-order model and Monod model were used to evaluate the kinetic constants of the BMP assays. The results demonstrated that fresh inoculum was the best option to perform BMP tests. This was evidenced by highest enzyme activity (0.11 U/mL) and highest methane yields for cellulose (356 NmL CH4/gVS) as well as wheat straw (261 NmL CH4/gVS). Besides, high biodegradability (85.8% for cellulose and 61.3% for wheat straw) was also obtained when the fresh inoculum was used. Moreover, a kinetic evaluation showed that inoculum pre-incubation at 37°C... (More)
- Biochemical methane potential (BMP) tests were carried out to investigate the influence of inoculum pre-treatments (filtration and pre-incubation) on methane production from cellulose and wheat straw. First-order model and Monod model were used to evaluate the kinetic constants of the BMP assays. The results demonstrated that fresh inoculum was the best option to perform BMP tests. This was evidenced by highest enzyme activity (0.11 U/mL) and highest methane yields for cellulose (356 NmL CH4/gVS) as well as wheat straw (261 NmL CH4/gVS). Besides, high biodegradability (85.8% for cellulose and 61.3% for wheat straw) was also obtained when the fresh inoculum was used. Moreover, a kinetic evaluation showed that inoculum pre-incubation at 37°C or storage at 4°C introduced a lag-time whereas the effects on hydrolysis rate were less consequent. In summary, pre-treatments affected the enzyme activity of the inoculum, and further on, significantly influenced the methane production and the degradation kinetics of the investigated substrates. It is recommended that filtration of inoculum should be avoided unless in case too large particles therein. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8243543
- author
- Wang, Bing LU ; Strömberg, Sten LU ; Ivo Achu, Nges LU ; Nistor, Mihaela LU and Liu, Jing LU
- organization
- publishing date
- 2015-10-30
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Bioscience and Bioengineering
- publisher
- Elsevier
- external identifiers
-
- pmid:26526543
- scopus:84961253893
- pmid:26526543
- wos:000376695300013
- ISSN
- 1347-4421
- DOI
- 10.1016/j.jbiosc.2015.10.004
- language
- English
- LU publication?
- yes
- id
- d0b20681-a2bd-4bf5-91d7-6311c265f732 (old id 8243543)
- date added to LUP
- 2016-04-01 09:55:50
- date last changed
- 2022-04-19 20:58:28
@article{d0b20681-a2bd-4bf5-91d7-6311c265f732,
abstract = {{Biochemical methane potential (BMP) tests were carried out to investigate the influence of inoculum pre-treatments (filtration and pre-incubation) on methane production from cellulose and wheat straw. First-order model and Monod model were used to evaluate the kinetic constants of the BMP assays. The results demonstrated that fresh inoculum was the best option to perform BMP tests. This was evidenced by highest enzyme activity (0.11 U/mL) and highest methane yields for cellulose (356 NmL CH4/gVS) as well as wheat straw (261 NmL CH4/gVS). Besides, high biodegradability (85.8% for cellulose and 61.3% for wheat straw) was also obtained when the fresh inoculum was used. Moreover, a kinetic evaluation showed that inoculum pre-incubation at 37°C or storage at 4°C introduced a lag-time whereas the effects on hydrolysis rate were less consequent. In summary, pre-treatments affected the enzyme activity of the inoculum, and further on, significantly influenced the methane production and the degradation kinetics of the investigated substrates. It is recommended that filtration of inoculum should be avoided unless in case too large particles therein.}},
author = {{Wang, Bing and Strömberg, Sten and Ivo Achu, Nges and Nistor, Mihaela and Liu, Jing}},
issn = {{1347-4421}},
language = {{eng}},
month = {{10}},
publisher = {{Elsevier}},
series = {{Journal of Bioscience and Bioengineering}},
title = {{Impacts of inoculum pre-treatments on enzyme activity and biochemical methane potential.}},
url = {{http://dx.doi.org/10.1016/j.jbiosc.2015.10.004}},
doi = {{10.1016/j.jbiosc.2015.10.004}},
year = {{2015}},
}