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Microfluidic Particle Sorting in Concentrated Erythrocyte Suspensions

Holm, Stefan H. LU ; Zhang, Zunmin ; Beech, Jason P. LU ; Gompper, Gerhard ; Fedosov, Dmitry A. and Tegenfeldt, Jonas O. LU (2019) In Physical Review Applied 12(1).
Abstract

An important step in diagnostics is the isolation of specific cells and microorganisms of interest from blood. Since such bioparticles are often present at very low concentrations, throughput needs to be as high as possible. In addition, to ensure simplicity, a minimum of sample preparation is important. Therefore, sorting schemes that function for whole blood are highly desirable. Deterministic lateral displacement (DLD) devices have proven to be very precise and versatile in terms of a wide range of sorting parameters. To better understand how DLD devices perform for blood as the hematocrit increases, we carry out measurements and simulations for spherical particles in the micrometer range which move through DLD arrays for different... (More)

An important step in diagnostics is the isolation of specific cells and microorganisms of interest from blood. Since such bioparticles are often present at very low concentrations, throughput needs to be as high as possible. In addition, to ensure simplicity, a minimum of sample preparation is important. Therefore, sorting schemes that function for whole blood are highly desirable. Deterministic lateral displacement (DLD) devices have proven to be very precise and versatile in terms of a wide range of sorting parameters. To better understand how DLD devices perform for blood as the hematocrit increases, we carry out measurements and simulations for spherical particles in the micrometer range which move through DLD arrays for different flow velocities and hematocrits ranging from pure buffer to concentrated erythrocyte suspensions mimicking whole blood. We find that the separation function of the DLD array is sustained even though the blood cells introduce a shift in the trajectories and a significant dispersion for particles whose diameters are close to the critical size in the device. Simulations qualitatively replicate our experimental observations and help us identify fundamental mechanisms for the effect of hematocrit on the performance of the DLD device.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Physical Review Applied
volume
12
issue
1
article number
014051
publisher
American Physical Society
external identifiers
  • scopus:85073642503
ISSN
2331-7019
DOI
10.1103/PhysRevApplied.12.014051
language
English
LU publication?
yes
id
8284046b-64d9-4b3b-918b-bd3764f9c5e6
date added to LUP
2019-11-05 09:36:28
date last changed
2020-05-24 06:27:19
@article{8284046b-64d9-4b3b-918b-bd3764f9c5e6,
  abstract     = {<p>An important step in diagnostics is the isolation of specific cells and microorganisms of interest from blood. Since such bioparticles are often present at very low concentrations, throughput needs to be as high as possible. In addition, to ensure simplicity, a minimum of sample preparation is important. Therefore, sorting schemes that function for whole blood are highly desirable. Deterministic lateral displacement (DLD) devices have proven to be very precise and versatile in terms of a wide range of sorting parameters. To better understand how DLD devices perform for blood as the hematocrit increases, we carry out measurements and simulations for spherical particles in the micrometer range which move through DLD arrays for different flow velocities and hematocrits ranging from pure buffer to concentrated erythrocyte suspensions mimicking whole blood. We find that the separation function of the DLD array is sustained even though the blood cells introduce a shift in the trajectories and a significant dispersion for particles whose diameters are close to the critical size in the device. Simulations qualitatively replicate our experimental observations and help us identify fundamental mechanisms for the effect of hematocrit on the performance of the DLD device.</p>},
  author       = {Holm, Stefan H. and Zhang, Zunmin and Beech, Jason P. and Gompper, Gerhard and Fedosov, Dmitry A. and Tegenfeldt, Jonas O.},
  issn         = {2331-7019},
  language     = {eng},
  number       = {1},
  publisher    = {American Physical Society},
  series       = {Physical Review Applied},
  title        = {Microfluidic Particle Sorting in Concentrated Erythrocyte Suspensions},
  url          = {http://dx.doi.org/10.1103/PhysRevApplied.12.014051},
  doi          = {10.1103/PhysRevApplied.12.014051},
  volume       = {12},
  year         = {2019},
}