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Immunolocalization of Collagens (I and III) and Cartilage Oligomeric Matrix Protein in the Normal and Injured Equine Superficial Digital Flexor Tendon

Sodersten, Fredrik ; Hultenby, Kjell ; Heinegård, Dick LU ; Johnston, Christopher and Ekman, Stina (2013) In Connective Tissue Research 54(1). p.62-69
Abstract
This is a descriptive study of tendon pathology with different structural appearances of repair tissue correlated to immunolocalization of cartilage oligomeric matrix protein (COMP) and type I and III collagens and expression of COMP mRNA. The material consists of nine tendons from seven horses (5-25 years old; mean age of 10 years) with clinical tendinopathy and three normal tendons from horses (3, 3, and 13 years old) euthanized for non-orthopedic reasons. The injured tendons displayed different repair-tissue appearances with organized and disorganized fibroblastic regions as well as areas of necrosis. The normal tendons presented distinct immunoreactivity for COMP and expression of COMP mRNA and type I collagen in the normal aligned... (More)
This is a descriptive study of tendon pathology with different structural appearances of repair tissue correlated to immunolocalization of cartilage oligomeric matrix protein (COMP) and type I and III collagens and expression of COMP mRNA. The material consists of nine tendons from seven horses (5-25 years old; mean age of 10 years) with clinical tendinopathy and three normal tendons from horses (3, 3, and 13 years old) euthanized for non-orthopedic reasons. The injured tendons displayed different repair-tissue appearances with organized and disorganized fibroblastic regions as well as areas of necrosis. The normal tendons presented distinct immunoreactivity for COMP and expression of COMP mRNA and type I collagen in the normal aligned fiber structures, but no immunolabeling of type III collagen. However, immunoreactivity for type III collagen was present in the endotenon surrounding the fiber bundles, where no expression of COMP could be seen. Immunostaining for type I and III collagens was present in all of the pathologic regions indicating repair tissue. Interestingly, the granulation tissues showed immunostaining for COMP and expression of COMP mRNA, indicating a role for COMP in repair and remodeling of the tendon after fiber degeneration and rupture. The present results suggest that not only type III collagen but also COMP is involved in the repair and remodeling processes of the tendon. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
equine, tendon repair, immunolocalization, extracellular matrix (ECM)
in
Connective Tissue Research
volume
54
issue
1
pages
62 - 69
publisher
Taylor & Francis
external identifiers
  • wos:000313289900010
  • scopus:84872136816
  • pmid:23020676
ISSN
1607-8438
DOI
10.3109/03008207.2012.734879
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Connective Tissue Biology (013230151)
id
829030a2-a915-40d4-9378-efbc38e7b751 (old id 3476779)
date added to LUP
2016-04-01 11:06:24
date last changed
2022-01-26 05:27:36
@article{829030a2-a915-40d4-9378-efbc38e7b751,
  abstract     = {{This is a descriptive study of tendon pathology with different structural appearances of repair tissue correlated to immunolocalization of cartilage oligomeric matrix protein (COMP) and type I and III collagens and expression of COMP mRNA. The material consists of nine tendons from seven horses (5-25 years old; mean age of 10 years) with clinical tendinopathy and three normal tendons from horses (3, 3, and 13 years old) euthanized for non-orthopedic reasons. The injured tendons displayed different repair-tissue appearances with organized and disorganized fibroblastic regions as well as areas of necrosis. The normal tendons presented distinct immunoreactivity for COMP and expression of COMP mRNA and type I collagen in the normal aligned fiber structures, but no immunolabeling of type III collagen. However, immunoreactivity for type III collagen was present in the endotenon surrounding the fiber bundles, where no expression of COMP could be seen. Immunostaining for type I and III collagens was present in all of the pathologic regions indicating repair tissue. Interestingly, the granulation tissues showed immunostaining for COMP and expression of COMP mRNA, indicating a role for COMP in repair and remodeling of the tendon after fiber degeneration and rupture. The present results suggest that not only type III collagen but also COMP is involved in the repair and remodeling processes of the tendon.}},
  author       = {{Sodersten, Fredrik and Hultenby, Kjell and Heinegård, Dick and Johnston, Christopher and Ekman, Stina}},
  issn         = {{1607-8438}},
  keywords     = {{equine; tendon repair; immunolocalization; extracellular matrix (ECM)}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{62--69}},
  publisher    = {{Taylor & Francis}},
  series       = {{Connective Tissue Research}},
  title        = {{Immunolocalization of Collagens (I and III) and Cartilage Oligomeric Matrix Protein in the Normal and Injured Equine Superficial Digital Flexor Tendon}},
  url          = {{http://dx.doi.org/10.3109/03008207.2012.734879}},
  doi          = {{10.3109/03008207.2012.734879}},
  volume       = {{54}},
  year         = {{2013}},
}