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Label-free enrichment of primary human skeletal progenitor cells using deterministic lateral displacement

Xavier, Miguel ; Holm, Stefan H. LU ; Beech, Jason P. LU ; Spencer, Daniel ; Tegenfeldt, Jonas O. LU orcid ; Oreffo, Richard O.C. and Morgan, Hywel (2019) In Lab on a Chip 19(3). p.513-523
Abstract

Skeletal stem cells (SSCs) are present in bone marrow (BM) and offer great potential for bone regenerative therapies. However, in the absence of a unique marker, current sorting approaches remain challenging in the quest for simple strategies to deliver SSCs with consistent regeneration and differentiation capacities. Microfluidics offers the possibility to sort cells marker-free, based on intrinsic biophysical properties. Recent studies indicate that SSCs are stiffer than leukocytes and are contained within the larger cell fraction in BM. This paper describes the use of deterministic lateral displacement (DLD) to sort SSCs based on cell size and stiffness. DLD is a technology that uses arrays of micropillars to sort cells based on... (More)

Skeletal stem cells (SSCs) are present in bone marrow (BM) and offer great potential for bone regenerative therapies. However, in the absence of a unique marker, current sorting approaches remain challenging in the quest for simple strategies to deliver SSCs with consistent regeneration and differentiation capacities. Microfluidics offers the possibility to sort cells marker-free, based on intrinsic biophysical properties. Recent studies indicate that SSCs are stiffer than leukocytes and are contained within the larger cell fraction in BM. This paper describes the use of deterministic lateral displacement (DLD) to sort SSCs based on cell size and stiffness. DLD is a technology that uses arrays of micropillars to sort cells based on their diameter. Cell deformation within the device can change the cell size and affect sorting - here evidenced using human cell lines and by fractionation of expanded SSCs. Following sorting, SSCs remained viable and retained their capacity to form clonogenic cultures (CFU-F), indicative of stem cell potential. Additionally, larger BM cells showed enhanced capacity to form CFU-F. These findings support the theory that SSCs are more abundant within the larger BM cell fraction and that DLD, or other size-based approaches, could be used to provide enriched SSC populations with significant implications for stem cell research and translation to the clinic.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lab on a Chip
volume
19
issue
3
pages
11 pages
publisher
Royal Society of Chemistry
external identifiers
  • pmid:30632599
  • scopus:85060634478
ISSN
1473-0189
DOI
10.1039/c8lc01154k
language
English
LU publication?
yes
id
82bc438d-ce6c-48cb-bc0f-5e4d80b30c35
date added to LUP
2019-02-05 08:54:37
date last changed
2024-08-20 09:48:50
@article{82bc438d-ce6c-48cb-bc0f-5e4d80b30c35,
  abstract     = {{<p>Skeletal stem cells (SSCs) are present in bone marrow (BM) and offer great potential for bone regenerative therapies. However, in the absence of a unique marker, current sorting approaches remain challenging in the quest for simple strategies to deliver SSCs with consistent regeneration and differentiation capacities. Microfluidics offers the possibility to sort cells marker-free, based on intrinsic biophysical properties. Recent studies indicate that SSCs are stiffer than leukocytes and are contained within the larger cell fraction in BM. This paper describes the use of deterministic lateral displacement (DLD) to sort SSCs based on cell size and stiffness. DLD is a technology that uses arrays of micropillars to sort cells based on their diameter. Cell deformation within the device can change the cell size and affect sorting - here evidenced using human cell lines and by fractionation of expanded SSCs. Following sorting, SSCs remained viable and retained their capacity to form clonogenic cultures (CFU-F), indicative of stem cell potential. Additionally, larger BM cells showed enhanced capacity to form CFU-F. These findings support the theory that SSCs are more abundant within the larger BM cell fraction and that DLD, or other size-based approaches, could be used to provide enriched SSC populations with significant implications for stem cell research and translation to the clinic.</p>}},
  author       = {{Xavier, Miguel and Holm, Stefan H. and Beech, Jason P. and Spencer, Daniel and Tegenfeldt, Jonas O. and Oreffo, Richard O.C. and Morgan, Hywel}},
  issn         = {{1473-0189}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{513--523}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{Lab on a Chip}},
  title        = {{Label-free enrichment of primary human skeletal progenitor cells using deterministic lateral displacement}},
  url          = {{http://dx.doi.org/10.1039/c8lc01154k}},
  doi          = {{10.1039/c8lc01154k}},
  volume       = {{19}},
  year         = {{2019}},
}