In vivo measurements of diffuse reflectance and time-resolved autofluorescence emission spectra of basal cell carcinomas
(2012) In Journal of Biophotonics 5(3). p.240-254- Abstract
- We present a clinical investigation of diffuse reflectance and time-resolved autofluorescence spectra of skin cancer with an emphasis on basal cell carcinoma. A total of 25 patients were measured using a compact steady-state diffuse reflectance/fluorescence spectrometer and a fibre-optic-coupled multispectral time-resolved spectrofluorometer. Measurements were performed in vivo prior to surgical excision of the investigated region. Singular value decomposition was used to reduce the dimensionality of steady state diffuse reflectance and fluorescence spectra. Linear discriminant analysis was then applied to the measurements of basal cell carcinomas (BCCs) and used to predict the tissue disease state with a leave-one-out methodology. This... (More)
- We present a clinical investigation of diffuse reflectance and time-resolved autofluorescence spectra of skin cancer with an emphasis on basal cell carcinoma. A total of 25 patients were measured using a compact steady-state diffuse reflectance/fluorescence spectrometer and a fibre-optic-coupled multispectral time-resolved spectrofluorometer. Measurements were performed in vivo prior to surgical excision of the investigated region. Singular value decomposition was used to reduce the dimensionality of steady state diffuse reflectance and fluorescence spectra. Linear discriminant analysis was then applied to the measurements of basal cell carcinomas (BCCs) and used to predict the tissue disease state with a leave-one-out methodology. This approach was able to correctly diagnose 87% of the BCCs. With 445 nm excitation a decrease in the spectrally averaged fluorescence lifetime was observed between normal tissue and BCC lesions with a mean value of 886 ps. Furthermore, the fluorescence lifetime for BCCs was lower than that of the surrounding healthy tissue in all cases and statistical analysis of the data revealed that this decrease was significant (p = 0.002). (C) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2379177
- author
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- fluorescence, fluorescence lifetime, diffuse reflectance, skin cancer, spectrometer, fibre probe
- in
- Journal of Biophotonics
- volume
- 5
- issue
- 3
- pages
- 240 - 254
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000301053900008
- scopus:84857976890
- pmid:22308093
- ISSN
- 1864-063X
- DOI
- 10.1002/jbio.201100126
- language
- English
- LU publication?
- yes
- id
- 82fc79f5-a950-40ed-819b-745d3ba6a063 (old id 2379177)
- date added to LUP
- 2016-04-01 10:51:53
- date last changed
- 2022-04-28 02:02:20
@misc{82fc79f5-a950-40ed-819b-745d3ba6a063,
abstract = {{We present a clinical investigation of diffuse reflectance and time-resolved autofluorescence spectra of skin cancer with an emphasis on basal cell carcinoma. A total of 25 patients were measured using a compact steady-state diffuse reflectance/fluorescence spectrometer and a fibre-optic-coupled multispectral time-resolved spectrofluorometer. Measurements were performed in vivo prior to surgical excision of the investigated region. Singular value decomposition was used to reduce the dimensionality of steady state diffuse reflectance and fluorescence spectra. Linear discriminant analysis was then applied to the measurements of basal cell carcinomas (BCCs) and used to predict the tissue disease state with a leave-one-out methodology. This approach was able to correctly diagnose 87% of the BCCs. With 445 nm excitation a decrease in the spectrally averaged fluorescence lifetime was observed between normal tissue and BCC lesions with a mean value of 886 ps. Furthermore, the fluorescence lifetime for BCCs was lower than that of the surrounding healthy tissue in all cases and statistical analysis of the data revealed that this decrease was significant (p = 0.002). (C) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)}},
author = {{Thompson, Alex J. and Coda, Sergio and Brydegaard, Mikkel and Kennedy, Gordon and Patalay, Rakesh and Waitong-Bramming, Ulrika and De Beule, Pieter A. A. and Neil, Mark A. A. and Andersson-Engels, Stefan and Bendsöe, Niels and French, Paul M. W. and Svanberg, Katarina and Dunsby, Chris}},
issn = {{1864-063X}},
keywords = {{fluorescence; fluorescence lifetime; diffuse reflectance; skin cancer; spectrometer; fibre probe}},
language = {{eng}},
number = {{3}},
pages = {{240--254}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Biophotonics}},
title = {{In vivo measurements of diffuse reflectance and time-resolved autofluorescence emission spectra of basal cell carcinomas}},
url = {{http://dx.doi.org/10.1002/jbio.201100126}},
doi = {{10.1002/jbio.201100126}},
volume = {{5}},
year = {{2012}},
}