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Ex vivo dual perfusion of an isolated human placenta cotyledon : Towards protocol standardization and improved inter-centre comparability

Schneider, Henning ; Albrecht, Christiane ; Ahmed, Mahmoud S. ; Broekhuizen, Michelle ; Aengenheister, Leonie ; Buerki-Thurnherr, Tina ; Danser, A. H.Jan ; Gil, Sophie ; Hansson, Stefan R. LU orcid and Greupink, Rick , et al. (2022) In Placenta 126. p.83-89
Abstract

Since the full development of the ex vivo dual perfusion model of the human placenta cotyledon, the technique has provided essential insight into how nutrients, lipids, gases, immunoglobulins, endocrine agents, pharmaceuticals, chemicals, nanoparticles, micro-organisms and parasites might traverse the maternofetal barrier. Additionally, the model has been instrumental in gaining a better understanding of the regulation of vascular tone, endocrinology and metabolism within this organ. The human placenta is unique amongst species in its anatomy and transfer modalities. This orthologous diversity therefore requires an appropriate consideration of placental transfer rates of compounds, particles and micro-organisms specific to humans.... (More)

Since the full development of the ex vivo dual perfusion model of the human placenta cotyledon, the technique has provided essential insight into how nutrients, lipids, gases, immunoglobulins, endocrine agents, pharmaceuticals, chemicals, nanoparticles, micro-organisms and parasites might traverse the maternofetal barrier. Additionally, the model has been instrumental in gaining a better understanding of the regulation of vascular tone, endocrinology and metabolism within this organ. The human placenta is unique amongst species in its anatomy and transfer modalities. This orthologous diversity therefore requires an appropriate consideration of placental transfer rates of compounds, particles and micro-organisms specific to humans. Different research centres have adapted this model with a wide variation in perfusion parameters, including in the establishment of perfusion, perfusate composition, gassing regime, cannulation method, flow rates, perfused tissue mass, and also in the application of quality control measures. The requirement to harmonise and standardise perfusion practice between centres is largely driven by the need to obtain consistency in our understanding of placental function, but also in the qualification of the model for acceptance by regulatory agencies in drug and toxicology testing. A pilot study is proposed, aiming to describe how existing inter-centre variation in perfusion methodology affects placental metabolism, protein synthesis, oxygen consumption, the materno-fetal transfer of key molecular markers, and placental structure.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Function, Perfusion, Placenta, Regulatory, Standardisation, Transfer
in
Placenta
volume
126
pages
7 pages
publisher
W.B. Saunders
external identifiers
  • pmid:35785693
  • scopus:85133237700
ISSN
0143-4004
DOI
10.1016/j.placenta.2022.05.003
language
English
LU publication?
yes
id
839226f1-a01f-4e72-b793-fb31ba87bf1d
date added to LUP
2022-10-04 11:22:14
date last changed
2024-06-13 16:03:06
@article{839226f1-a01f-4e72-b793-fb31ba87bf1d,
  abstract     = {{<p>Since the full development of the ex vivo dual perfusion model of the human placenta cotyledon, the technique has provided essential insight into how nutrients, lipids, gases, immunoglobulins, endocrine agents, pharmaceuticals, chemicals, nanoparticles, micro-organisms and parasites might traverse the maternofetal barrier. Additionally, the model has been instrumental in gaining a better understanding of the regulation of vascular tone, endocrinology and metabolism within this organ. The human placenta is unique amongst species in its anatomy and transfer modalities. This orthologous diversity therefore requires an appropriate consideration of placental transfer rates of compounds, particles and micro-organisms specific to humans. Different research centres have adapted this model with a wide variation in perfusion parameters, including in the establishment of perfusion, perfusate composition, gassing regime, cannulation method, flow rates, perfused tissue mass, and also in the application of quality control measures. The requirement to harmonise and standardise perfusion practice between centres is largely driven by the need to obtain consistency in our understanding of placental function, but also in the qualification of the model for acceptance by regulatory agencies in drug and toxicology testing. A pilot study is proposed, aiming to describe how existing inter-centre variation in perfusion methodology affects placental metabolism, protein synthesis, oxygen consumption, the materno-fetal transfer of key molecular markers, and placental structure.</p>}},
  author       = {{Schneider, Henning and Albrecht, Christiane and Ahmed, Mahmoud S. and Broekhuizen, Michelle and Aengenheister, Leonie and Buerki-Thurnherr, Tina and Danser, A. H.Jan and Gil, Sophie and Hansson, Stefan R. and Greupink, Rick and Lewis, Rohan M. and Markert, Udo R. and Mathiesen, Line and Powles-Glover, Nicola and Wadsack, Christian and Brownbill, Paul}},
  issn         = {{0143-4004}},
  keywords     = {{Function; Perfusion; Placenta; Regulatory; Standardisation; Transfer}},
  language     = {{eng}},
  pages        = {{83--89}},
  publisher    = {{W.B. Saunders}},
  series       = {{Placenta}},
  title        = {{Ex vivo dual perfusion of an isolated human placenta cotyledon : Towards protocol standardization and improved inter-centre comparability}},
  url          = {{http://dx.doi.org/10.1016/j.placenta.2022.05.003}},
  doi          = {{10.1016/j.placenta.2022.05.003}},
  volume       = {{126}},
  year         = {{2022}},
}