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BioMAX the first macromolecular crystallography beamline at MAX IV Laboratory

Ursby, Thomas LU ; Åhnberg, Karl LU ; Appio, Roberto LU ; Aurelius, Oskar LU ; Barczyk, Artur LU ; Bartalesi, Antonio LU ; Bjelcic, Monika LU ; Bolmsten, Fredrik LU ; Cerenius, Yngve LU and Doak, R. Bruce , et al. (2020) In Journal of Synchrotron Radiation 27. p.1415-1429
Abstract

BioMAX is the first macromolecular crystallography beamline at the MAX IV Laboratory 3 GeV storage ring, which is the first operational multi-bend achromat storage ring. Due to the low-emittance storage ring, BioMAX has a parallel, high-intensity X-ray beam, even when focused down to 20 μm × 5 μm using the bendable focusing mirrors. The beam is tunable in the energy range 5-25 keV using the in-vacuum undulator and the horizontally deflecting double-crystal monochromator. BioMAX is equipped with an MD3 diffractometer, an ISARA high-capacity sample changer and an EIGER 16M hybrid pixel detector. Data collection at BioMAX is controlled using the newly developed MXCuBE3 graphical user interface, and sample tracking is handled by ISPyB. The... (More)

BioMAX is the first macromolecular crystallography beamline at the MAX IV Laboratory 3 GeV storage ring, which is the first operational multi-bend achromat storage ring. Due to the low-emittance storage ring, BioMAX has a parallel, high-intensity X-ray beam, even when focused down to 20 μm × 5 μm using the bendable focusing mirrors. The beam is tunable in the energy range 5-25 keV using the in-vacuum undulator and the horizontally deflecting double-crystal monochromator. BioMAX is equipped with an MD3 diffractometer, an ISARA high-capacity sample changer and an EIGER 16M hybrid pixel detector. Data collection at BioMAX is controlled using the newly developed MXCuBE3 graphical user interface, and sample tracking is handled by ISPyB. The computing infrastructure includes data storage and processing both at MAX IV and the Lund University supercomputing center LUNARC. With state-of-the-art instrumentation, a high degree of automation, a user-friendly control system interface and remote operation, BioMAX provides an excellent facility for most macromolecular crystallography experiments. Serial crystallography using either a high-viscosity extruder injector or the MD3 as a fixed-target scanner is already implemented. The serial crystallography activities at MAX IV Laboratory will be further developed at the microfocus beamline MicroMAX, when it comes into operation in 2022. MicroMAX will have a 1 μm × 1 μm beam focus and a flux up to 1015 photons s with main applications in serial crystallography, room-temperature structure determinations and time-resolved experiments.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
automation, beamline, macromolecular crystallography, MBA, micro-focus, remote operation, serial crystallography
in
Journal of Synchrotron Radiation
volume
27
pages
15 pages
publisher
International Union of Crystallography
external identifiers
  • scopus:85090284348
  • pmid:32876619
ISSN
0909-0495
DOI
10.1107/S1600577520008723
language
English
LU publication?
yes
id
8497a985-59c0-4507-94c3-fa4bb586a682
date added to LUP
2021-01-12 13:49:17
date last changed
2021-03-03 05:08:13
@article{8497a985-59c0-4507-94c3-fa4bb586a682,
  abstract     = {<p>BioMAX is the first macromolecular crystallography beamline at the MAX IV Laboratory 3 GeV storage ring, which is the first operational multi-bend achromat storage ring. Due to the low-emittance storage ring, BioMAX has a parallel, high-intensity X-ray beam, even when focused down to 20 μm × 5 μm using the bendable focusing mirrors. The beam is tunable in the energy range 5-25 keV using the in-vacuum undulator and the horizontally deflecting double-crystal monochromator. BioMAX is equipped with an MD3 diffractometer, an ISARA high-capacity sample changer and an EIGER 16M hybrid pixel detector. Data collection at BioMAX is controlled using the newly developed MXCuBE3 graphical user interface, and sample tracking is handled by ISPyB. The computing infrastructure includes data storage and processing both at MAX IV and the Lund University supercomputing center LUNARC. With state-of-the-art instrumentation, a high degree of automation, a user-friendly control system interface and remote operation, BioMAX provides an excellent facility for most macromolecular crystallography experiments. Serial crystallography using either a high-viscosity extruder injector or the MD3 as a fixed-target scanner is already implemented. The serial crystallography activities at MAX IV Laboratory will be further developed at the microfocus beamline MicroMAX, when it comes into operation in 2022. MicroMAX will have a 1 μm × 1 μm beam focus and a flux up to 1015 photons s with main applications in serial crystallography, room-temperature structure determinations and time-resolved experiments.</p>},
  author       = {Ursby, Thomas and Åhnberg, Karl and Appio, Roberto and Aurelius, Oskar and Barczyk, Artur and Bartalesi, Antonio and Bjelcic, Monika and Bolmsten, Fredrik and Cerenius, Yngve and Doak, R. Bruce and Eguiraun, Mikel and Eriksson, Thomas and Friel, Ross J. and Gorgisyan, Ishkhan and Gross, Andrea and Haghighat, Vahid and Hennies, Franz and Jagudin, Elmir and Jensen, Brian Norsk and Jeppsson, Tobias and Kloos, Marco and Lidon-Simon, Julio and Lima, Gustavo M.A.De and Lizatovic, Robert and Lundin, Magnus and Milan-Otero, Antonio and Milas, Mirko and Nan, Jie and Nardella, Alberto and Rosborg, Anders and Shilova, Anastasiia and Shoeman, Robert L. and Siewert, Frank and Sondhauss, Peter and Talibov, Vladimir O. and Tarawneh, Hamed and Thånell, Johan and Thunnissen, Marjolein and Unge, Johan and Ward, Christopher and Gonzaleza, Ana and Muellera, Uwe},
  issn         = {0909-0495},
  language     = {eng},
  month        = {09},
  pages        = {1415--1429},
  publisher    = {International Union of Crystallography},
  series       = {Journal of Synchrotron Radiation},
  title        = {BioMAX the first macromolecular crystallography beamline at MAX IV Laboratory},
  url          = {http://dx.doi.org/10.1107/S1600577520008723},
  doi          = {10.1107/S1600577520008723},
  volume       = {27},
  year         = {2020},
}