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Unlike VIP, the VIP-related peptides PACAP, helodermin and helospectin suppress electrically evoked contractions of rat vas deferens

Grundemar, L LU and Håkanson, R LU (1992) In Regulatory Peptides 40(3). p.331-337
Abstract

We have compared the effects of vasoactive intestinal peptide (VIP) and of the VIP-related peptides pituitary adenylate cyclase activating peptide (PACAP) 1-27 and 1-38, helodermin, helospectin I and helospectin II, on the electrically evoked twitches in the isolated vas deferens of the rat. While VIP was virtually without effect, PACAP 1-38 suppressed the electrically evoked twitches effectively and in a concentration-dependent manner (pIC50 value 7.5). The naturally occurring N-terminal fragment PACAP 1-27 was less effective than PACAP 1-38 (Imax values 37.2% suppression compared to 76.5%) and less potent. The C-terminal fragment PACAP 16-38 was virtually inactive. Also helodermin and helospectin I+II suppressed the electrically... (More)

We have compared the effects of vasoactive intestinal peptide (VIP) and of the VIP-related peptides pituitary adenylate cyclase activating peptide (PACAP) 1-27 and 1-38, helodermin, helospectin I and helospectin II, on the electrically evoked twitches in the isolated vas deferens of the rat. While VIP was virtually without effect, PACAP 1-38 suppressed the electrically evoked twitches effectively and in a concentration-dependent manner (pIC50 value 7.5). The naturally occurring N-terminal fragment PACAP 1-27 was less effective than PACAP 1-38 (Imax values 37.2% suppression compared to 76.5%) and less potent. The C-terminal fragment PACAP 16-38 was virtually inactive. Also helodermin and helospectin I+II suppressed the electrically evoked twitches effectively and in a concentration-dependent manner (pIC50 values 6.9; 7.2; 6.8, respectively). The three peptides produced similar maximum reduction of the twitches (74-80%). The findings suggest that PACAP, helodermin and helospectin suppress the electrically evoked contractions in the rat vas deferens via receptors distinct from VIP receptors.

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keywords
Amino Acid Sequence, Animals, Electric Stimulation, In Vitro Techniques, Male, Molecular Sequence Data, Muscle Contraction/drug effects, Muscle, Smooth/drug effects, Neuropeptides/pharmacology, Peptides/pharmacology, Pituitary Adenylate Cyclase-Activating Polypeptide, Rats, Rats, Sprague-Dawley, Vas Deferens/drug effects, Vasoactive Intestinal Peptide/pharmacology
in
Regulatory Peptides
volume
40
issue
3
pages
331 - 337
publisher
Elsevier
external identifiers
  • pmid:1438978
  • scopus:0026808923
ISSN
0167-0115
DOI
10.1016/0167-0115(92)90520-5
language
English
LU publication?
yes
id
84d24b03-427a-4f0d-a358-1cf58278104c
date added to LUP
2019-09-03 14:09:48
date last changed
2020-01-13 02:19:31
@article{84d24b03-427a-4f0d-a358-1cf58278104c,
  abstract     = {<p>We have compared the effects of vasoactive intestinal peptide (VIP) and of the VIP-related peptides pituitary adenylate cyclase activating peptide (PACAP) 1-27 and 1-38, helodermin, helospectin I and helospectin II, on the electrically evoked twitches in the isolated vas deferens of the rat. While VIP was virtually without effect, PACAP 1-38 suppressed the electrically evoked twitches effectively and in a concentration-dependent manner (pIC50 value 7.5). The naturally occurring N-terminal fragment PACAP 1-27 was less effective than PACAP 1-38 (Imax values 37.2% suppression compared to 76.5%) and less potent. The C-terminal fragment PACAP 16-38 was virtually inactive. Also helodermin and helospectin I+II suppressed the electrically evoked twitches effectively and in a concentration-dependent manner (pIC50 values 6.9; 7.2; 6.8, respectively). The three peptides produced similar maximum reduction of the twitches (74-80%). The findings suggest that PACAP, helodermin and helospectin suppress the electrically evoked contractions in the rat vas deferens via receptors distinct from VIP receptors.</p>},
  author       = {Grundemar, L and Håkanson, R},
  issn         = {0167-0115},
  language     = {eng},
  month        = {08},
  number       = {3},
  pages        = {331--337},
  publisher    = {Elsevier},
  series       = {Regulatory Peptides},
  title        = {Unlike VIP, the VIP-related peptides PACAP, helodermin and helospectin suppress electrically evoked contractions of rat vas deferens},
  url          = {http://dx.doi.org/10.1016/0167-0115(92)90520-5},
  doi          = {10.1016/0167-0115(92)90520-5},
  volume       = {40},
  year         = {1992},
}