A whole blood approach improves speed and accuracy when measuring mitochondrial respiration in intact avian blood cells
Nord, Andreas LU ; Chamkha, Imen LU and Elmer, Eskil LU
(2023)
In FASEB Journal
37(3).
- Abstract (Swedish)
- Understanding mitochondrial biology and pathology is key to understanding the evolution of animal form and function. However, mitochondrial measurement often involves invasive, or even terminal, sampling, which can be difficult to reconcile in wild models or longitudinal studies. Non-mammal vertebrates contain mitochondria in their red blood cells, which can be exploited for minimally invasive mitochondrial measurement. Several recent bird studies have measured mitochondrial function using isolated blood cells. Isolation adds time in the laboratory and might be associated with physiological complications. We developed and validated a protocol to measure mitochondrial respiration in bird whole blood. Endogenous respiration was comparable... (More)
- Understanding mitochondrial biology and pathology is key to understanding the evolution of animal form and function. However, mitochondrial measurement often involves invasive, or even terminal, sampling, which can be difficult to reconcile in wild models or longitudinal studies. Non-mammal vertebrates contain mitochondria in their red blood cells, which can be exploited for minimally invasive mitochondrial measurement. Several recent bird studies have measured mitochondrial function using isolated blood cells. Isolation adds time in the laboratory and might be associated with physiological complications. We developed and validated a protocol to measure mitochondrial respiration in bird whole blood. Endogenous respiration was comparable between isolated blood cells and whole blood. However, respiration towards oxidative phosphorylation was higher in whole blood, and whole blood mitochondria were better coupled and had higher maximum working capacity. Whole blood measurement was also more reproducible than measurement on isolated cells for all traits considered. Measurements were feasible over a 10-fold range of sample volumes, although both small and large volumes were associated with changes to respiratory traits. The protocol was compatible with long-term storage: after 24 h at 5°C without agitation, all respiration traits but maximum working capacity remained unchanged, the latter decreasing by 14%. Our study suggests that whole blood measurement provides faster, more reproducible, and more biologically and physiologically relevant (mitochondrial integrity) assessment of mitochondrial respiration. We recommend future studies to take a whole blood approach unless specific circumstances require the use of isolated blood cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/84f356a9-5d2f-4c47-91f2-d040a2c73b9e
- author
- Nord, Andreas
LU
; Chamkha, Imen
LU
and Elmer, Eskil
LU
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- blood, blood cells, cell respiration, energy, erythrocyte, metabolism, mitochondria, bird, zebra finch
- in
- FASEB Journal
- volume
- 37
- issue
- 3
- article number
- e22766
- pages
- 12 pages
- publisher
- Wiley
- external identifiers
-
- scopus:85147458113
- pmid:36734850
- ISSN
- 1530-6860
- DOI
- 10.1096/fj.202201749R
- language
- English
- LU publication?
- yes
- id
- 84f356a9-5d2f-4c47-91f2-d040a2c73b9e
- date added to LUP
- 2023-03-25 09:10:08
- date last changed
- 2024-05-16 12:22:44
@article{84f356a9-5d2f-4c47-91f2-d040a2c73b9e,
abstract = {{Understanding mitochondrial biology and pathology is key to understanding the evolution of animal form and function. However, mitochondrial measurement often involves invasive, or even terminal, sampling, which can be difficult to reconcile in wild models or longitudinal studies. Non-mammal vertebrates contain mitochondria in their red blood cells, which can be exploited for minimally invasive mitochondrial measurement. Several recent bird studies have measured mitochondrial function using isolated blood cells. Isolation adds time in the laboratory and might be associated with physiological complications. We developed and validated a protocol to measure mitochondrial respiration in bird whole blood. Endogenous respiration was comparable between isolated blood cells and whole blood. However, respiration towards oxidative phosphorylation was higher in whole blood, and whole blood mitochondria were better coupled and had higher maximum working capacity. Whole blood measurement was also more reproducible than measurement on isolated cells for all traits considered. Measurements were feasible over a 10-fold range of sample volumes, although both small and large volumes were associated with changes to respiratory traits. The protocol was compatible with long-term storage: after 24 h at 5°C without agitation, all respiration traits but maximum working capacity remained unchanged, the latter decreasing by 14%. Our study suggests that whole blood measurement provides faster, more reproducible, and more biologically and physiologically relevant (mitochondrial integrity) assessment of mitochondrial respiration. We recommend future studies to take a whole blood approach unless specific circumstances require the use of isolated blood cells.}},
author = {{Nord, Andreas and Chamkha, Imen and Elmer, Eskil}},
issn = {{1530-6860}},
keywords = {{blood; blood cells; cell respiration; energy; erythrocyte; metabolism; mitochondria; bird; zebra finch}},
language = {{eng}},
number = {{3}},
publisher = {{Wiley}},
series = {{FASEB Journal}},
title = {{A whole blood approach improves speed and accuracy when measuring mitochondrial respiration in intact avian blood cells}},
url = {{https://lup.lub.lu.se/search/files/141439271/Nord_et_al._FASEB_J_2023.pdf}},
doi = {{10.1096/fj.202201749R}},
volume = {{37}},
year = {{2023}},
}