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Association of tapasin and COPI provides a mechanism for the retrograde transport of major histocompatibility complex (MHC) class I molecules from the Golgi complex to the endoplasmic reticulum

Paulsson, Kajsa M LU orcid ; Kleijmeer, Monique J ; Griffith, Janice ; Jevon, Marc ; Chen, Shangwu ; Anderson, Per O LU ; Sjögren, Hans Olov LU ; Li, Su-Ling LU and Wang, Ping (2002) In Journal of Biological Chemistry 277(21). p.18266-18271
Abstract

Tapasin is a subunit of the transporter associated with antigen processing (TAP). It associates with the major histocompatibility complex (MHC) class I. We show that tapasin interacts with beta- and gamma-subunits of COPI coatomer. COPI retrieves membrane proteins from the Golgi network back to the endoplasmic reticulum (ER). The COPI subunit-associated tapasin also interacts with MHC class I molecules suggesting that tapasin acts as the cargo receptor for packing MHC class I molecules as cargo proteins into COPI-coated vesicles. In tapasin mutant cells, neither TAP nor MHC class I are detected in association with the COPI coatomer. Interestingly, tapasin-associated MHC class I molecules are antigenic peptide-receptive and detected in... (More)

Tapasin is a subunit of the transporter associated with antigen processing (TAP). It associates with the major histocompatibility complex (MHC) class I. We show that tapasin interacts with beta- and gamma-subunits of COPI coatomer. COPI retrieves membrane proteins from the Golgi network back to the endoplasmic reticulum (ER). The COPI subunit-associated tapasin also interacts with MHC class I molecules suggesting that tapasin acts as the cargo receptor for packing MHC class I molecules as cargo proteins into COPI-coated vesicles. In tapasin mutant cells, neither TAP nor MHC class I are detected in association with the COPI coatomer. Interestingly, tapasin-associated MHC class I molecules are antigenic peptide-receptive and detected in both the ER and the Golgi. Our data suggest that tapasin is required for the COPI vesicle-mediated retrograde transport of immature MHC class I molecules from the Golgi network to the ER.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Non-U.S. Gov't, Subcellular Fractions/metabolism, Support, Histocompatibility Antigens Class I/*metabolism, Immunoglobulins/*metabolism, Golgi Apparatus/*metabolism, Endoplasmic Reticulum/*metabolism, Coat Protein Complex I/*metabolism, Cell Line, Antiporters/*metabolism, Biological Transport
in
Journal of Biological Chemistry
volume
277
issue
21
pages
6 pages
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • wos:000175975800005
  • scopus:0037166298
  • pmid:11884415
ISSN
1083-351X
DOI
10.1074/jbc.M201388200
language
English
LU publication?
yes
id
85102695-807b-4022-b51b-54eec818806c (old id 106133)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11884415&dopt=Abstract
date added to LUP
2016-04-01 12:38:11
date last changed
2025-04-04 14:47:21
@article{85102695-807b-4022-b51b-54eec818806c,
  abstract     = {{<p>Tapasin is a subunit of the transporter associated with antigen processing (TAP). It associates with the major histocompatibility complex (MHC) class I. We show that tapasin interacts with beta- and gamma-subunits of COPI coatomer. COPI retrieves membrane proteins from the Golgi network back to the endoplasmic reticulum (ER). The COPI subunit-associated tapasin also interacts with MHC class I molecules suggesting that tapasin acts as the cargo receptor for packing MHC class I molecules as cargo proteins into COPI-coated vesicles. In tapasin mutant cells, neither TAP nor MHC class I are detected in association with the COPI coatomer. Interestingly, tapasin-associated MHC class I molecules are antigenic peptide-receptive and detected in both the ER and the Golgi. Our data suggest that tapasin is required for the COPI vesicle-mediated retrograde transport of immature MHC class I molecules from the Golgi network to the ER.</p>}},
  author       = {{Paulsson, Kajsa M and Kleijmeer, Monique J and Griffith, Janice and Jevon, Marc and Chen, Shangwu and Anderson, Per O and Sjögren, Hans Olov and Li, Su-Ling and Wang, Ping}},
  issn         = {{1083-351X}},
  keywords     = {{Non-U.S. Gov't; Subcellular Fractions/metabolism; Support; Histocompatibility Antigens Class I/*metabolism; Immunoglobulins/*metabolism; Golgi Apparatus/*metabolism; Endoplasmic Reticulum/*metabolism; Coat Protein Complex I/*metabolism; Cell Line; Antiporters/*metabolism; Biological Transport}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{21}},
  pages        = {{18266--18271}},
  publisher    = {{American Society for Biochemistry and Molecular Biology}},
  series       = {{Journal of Biological Chemistry}},
  title        = {{Association of tapasin and COPI provides a mechanism for the retrograde transport of major histocompatibility complex (MHC) class I molecules from the Golgi complex to the endoplasmic reticulum}},
  url          = {{http://dx.doi.org/10.1074/jbc.M201388200}},
  doi          = {{10.1074/jbc.M201388200}},
  volume       = {{277}},
  year         = {{2002}},
}