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Photoconjugation of Molecularly Imprinted Polymer Nanoparticles for Surface-Enhanced Raman Detection of Propranolol

Kamra, Tripta LU ; Xu, Changgang LU ; Montelius, Lars LU ; Schnadt, Joachim LU ; Wijesundera, Samurdhi A.; Yan, Mingdi and Ye, Lei LU (2015) In ACS Applied Materials and Interfaces 7(49). p.27479-27485
Abstract
We report a simple and versatile method to covalently immobilize molecularly imprinted polymer (MIP) nanoparticles on a Raman active substrate (Klarite) using a disulfide-derivatized perfluorophenylazide (PFPA-disulfide). Gold-coated Klarite was functionalized with PFPA-disulfide via a gold sulfur bond. Upon light radiation, the available azido groups were converted to highly reactive singlet perfluorophenyl nitrene that undergoes a CH insertion reaction and form covalent bonds with the MIP nanoparticles. The resulting surfaces were characterized using scanning electron microscopy and surface enhanced Raman spectroscopy to study the morphology and template affinity of the surfaces, respectively. The Raman measurements clearly show a... (More)
We report a simple and versatile method to covalently immobilize molecularly imprinted polymer (MIP) nanoparticles on a Raman active substrate (Klarite) using a disulfide-derivatized perfluorophenylazide (PFPA-disulfide). Gold-coated Klarite was functionalized with PFPA-disulfide via a gold sulfur bond. Upon light radiation, the available azido groups were converted to highly reactive singlet perfluorophenyl nitrene that undergoes a CH insertion reaction and form covalent bonds with the MIP nanoparticles. The resulting surfaces were characterized using scanning electron microscopy and surface enhanced Raman spectroscopy to study the morphology and template affinity of the surfaces, respectively. The Raman measurements clearly show a dose-responsive signal when propranolol binds to the MIP surface. Because the MIP particles were covalently attached to the Raman active substrate, the sensing surface was stable and could be reused after regeneration in acetic acid solution. The MIP-based Raman sensor was used successfully to detect propranolol in urine samples (7.7 X 10(-4) M). Our results show that the high selectivity of MLPs and the fingerprint Raman identification can be integrated into a compact sensing unit using high-efficiency photoconjugation. Thus, the method proposed is reliable, efficient and fast for fabricating label-free chemical sensors. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
molecularly imprinted polymers, propranolol, perfluorophenylazide, disulfide, photocoupling, Klarite, surface-enhanced Raman spectroscopy, urine
in
ACS Applied Materials and Interfaces
volume
7
issue
49
pages
27479 - 27485
publisher
The American Chemical Society
external identifiers
  • wos:000366873900054
  • scopus:84950269220
ISSN
1944-8244
DOI
10.1021/acsami.5b09500
language
English
LU publication?
yes
id
bca76f86-115d-46a8-82d8-c4e3a7a9fe98 (old id 8548816)
date added to LUP
2016-01-28 10:33:02
date last changed
2017-07-02 03:11:10
@article{bca76f86-115d-46a8-82d8-c4e3a7a9fe98,
  abstract     = {We report a simple and versatile method to covalently immobilize molecularly imprinted polymer (MIP) nanoparticles on a Raman active substrate (Klarite) using a disulfide-derivatized perfluorophenylazide (PFPA-disulfide). Gold-coated Klarite was functionalized with PFPA-disulfide via a gold sulfur bond. Upon light radiation, the available azido groups were converted to highly reactive singlet perfluorophenyl nitrene that undergoes a CH insertion reaction and form covalent bonds with the MIP nanoparticles. The resulting surfaces were characterized using scanning electron microscopy and surface enhanced Raman spectroscopy to study the morphology and template affinity of the surfaces, respectively. The Raman measurements clearly show a dose-responsive signal when propranolol binds to the MIP surface. Because the MIP particles were covalently attached to the Raman active substrate, the sensing surface was stable and could be reused after regeneration in acetic acid solution. The MIP-based Raman sensor was used successfully to detect propranolol in urine samples (7.7 X 10(-4) M). Our results show that the high selectivity of MLPs and the fingerprint Raman identification can be integrated into a compact sensing unit using high-efficiency photoconjugation. Thus, the method proposed is reliable, efficient and fast for fabricating label-free chemical sensors.},
  author       = {Kamra, Tripta and Xu, Changgang and Montelius, Lars and Schnadt, Joachim and Wijesundera, Samurdhi A. and Yan, Mingdi and Ye, Lei},
  issn         = {1944-8244},
  keyword      = {molecularly imprinted polymers,propranolol,perfluorophenylazide,disulfide,photocoupling,Klarite,surface-enhanced Raman spectroscopy,urine},
  language     = {eng},
  number       = {49},
  pages        = {27479--27485},
  publisher    = {The American Chemical Society},
  series       = {ACS Applied Materials and Interfaces},
  title        = {Photoconjugation of Molecularly Imprinted Polymer Nanoparticles for Surface-Enhanced Raman Detection of Propranolol},
  url          = {http://dx.doi.org/10.1021/acsami.5b09500},
  volume       = {7},
  year         = {2015},
}