Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

In situ 13C solid-state polarization transfer NMR to follow starch transformations in food

Nowacka-Perrin, Agnieszka LU ; Steglich, Thomas ; Topgaard, Daniel LU and Bernin, Diana (2022) In Magnetic Resonance in Chemistry 60(7). p.671-677
Abstract

Convenience food products tend to alter their quality and texture while stored. Texture-giving food components are often starch-rich ingredients, such as pasta or rice. Starch transforms depending on time, temperature and water content, which alters the properties of products. Monitoring these transformations, which are associated with a change in mobility of the starch chain segments, could optimize the quality of food products containing multiple ingredients. In order to do so, we applied a simple and efficient in situ 13C solid-state magic angle spinning (MAS) NMR approach, based on two different polarization transfer schemes, cross polarization (CP) and insensitive nuclei enhanced by polarization transfer (INEPT). The... (More)

Convenience food products tend to alter their quality and texture while stored. Texture-giving food components are often starch-rich ingredients, such as pasta or rice. Starch transforms depending on time, temperature and water content, which alters the properties of products. Monitoring these transformations, which are associated with a change in mobility of the starch chain segments, could optimize the quality of food products containing multiple ingredients. In order to do so, we applied a simple and efficient in situ 13C solid-state magic angle spinning (MAS) NMR approach, based on two different polarization transfer schemes, cross polarization (CP) and insensitive nuclei enhanced by polarization transfer (INEPT). The efficiency of the CP and INEPT transfer depends strongly on the mobility of chain segments—the time scale of reorientation of the CH-bond and the order parameter. Rigid crystalline or amorphous starch chains give rise to CP peaks, whereas mobile gelatinized starch chains appear as INEPT peaks. Comparing 13C solid-state MAS NMR experiments based on CP and INEPT allows insight into the progress of gelatinization, and other starch transformations, by reporting on both rigid and mobile starch chains simultaneously with atomic resolution by the 13C chemical shift. In conjunction with 1H solid-state MAS NMR, complementary information about other food components present at low concentration, such as lipids and protein, can be obtained. We demonstrate our approach on starch-based products and commercial pasta as a function of temperature and storage.

(Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
C, H, convenience food, CP, gelatinization, INEPT, MAS, solid-state NMR, starch-rich food products
in
Magnetic Resonance in Chemistry
volume
60
issue
7
pages
671 - 677
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:35094442
  • scopus:85124547642
ISSN
0749-1581
DOI
10.1002/mrc.5253
language
English
LU publication?
yes
id
85636d78-c082-4d8b-a789-8298944db3f0
date added to LUP
2022-05-20 10:59:06
date last changed
2024-04-18 07:08:23
@article{85636d78-c082-4d8b-a789-8298944db3f0,
  abstract     = {{<p>Convenience food products tend to alter their quality and texture while stored. Texture-giving food components are often starch-rich ingredients, such as pasta or rice. Starch transforms depending on time, temperature and water content, which alters the properties of products. Monitoring these transformations, which are associated with a change in mobility of the starch chain segments, could optimize the quality of food products containing multiple ingredients. In order to do so, we applied a simple and efficient in situ <sup>13</sup>C solid-state magic angle spinning (MAS) NMR approach, based on two different polarization transfer schemes, cross polarization (CP) and insensitive nuclei enhanced by polarization transfer (INEPT). The efficiency of the CP and INEPT transfer depends strongly on the mobility of chain segments—the time scale of reorientation of the CH-bond and the order parameter. Rigid crystalline or amorphous starch chains give rise to CP peaks, whereas mobile gelatinized starch chains appear as INEPT peaks. Comparing <sup>13</sup>C solid-state MAS NMR experiments based on CP and INEPT allows insight into the progress of gelatinization, and other starch transformations, by reporting on both rigid and mobile starch chains simultaneously with atomic resolution by the <sup>13</sup>C chemical shift. In conjunction with <sup>1</sup>H solid-state MAS NMR, complementary information about other food components present at low concentration, such as lipids and protein, can be obtained. We demonstrate our approach on starch-based products and commercial pasta as a function of temperature and storage.</p>}},
  author       = {{Nowacka-Perrin, Agnieszka and Steglich, Thomas and Topgaard, Daniel and Bernin, Diana}},
  issn         = {{0749-1581}},
  keywords     = {{C; H; convenience food; CP; gelatinization; INEPT; MAS; solid-state NMR; starch-rich food products}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{671--677}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Magnetic Resonance in Chemistry}},
  title        = {{In situ <sup>13</sup>C solid-state polarization transfer NMR to follow starch transformations in food}},
  url          = {{http://dx.doi.org/10.1002/mrc.5253}},
  doi          = {{10.1002/mrc.5253}},
  volume       = {{60}},
  year         = {{2022}},
}