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Structural studies of hydrated samples of amorphous calcium phosphate and phosphoprotein nanoclusters.

Lenton, Samuel; Nylander, Tommy LU ; Holt, Carl; Sawyer, Lindsay; Härtlein, Michael; Müller, Harrald and Teixeira, Susana C M (2016) In European biophysics journal : EBJ
Abstract
There are abundant examples of nanoclusters and inorganic microcrystals in biology. Their study under physiologically relevant conditions remains challenging due to their heterogeneity, instability, and the requirements of sample preparation. Advantages of using neutron diffraction and contrast matching to characterize biomaterials are highlighted in this article. We have applied these and complementary techniques to search for nanocrystals within clusters of calcium phosphate sequestered by bovine phosphopeptides, derived from osteopontin or casein. The neutron diffraction patterns show broad features that could be consistent with hexagonal hydroxyapatite crystallites smaller than 18.9 Å. Such nanocrystallites are, however, undetected by... (More)
There are abundant examples of nanoclusters and inorganic microcrystals in biology. Their study under physiologically relevant conditions remains challenging due to their heterogeneity, instability, and the requirements of sample preparation. Advantages of using neutron diffraction and contrast matching to characterize biomaterials are highlighted in this article. We have applied these and complementary techniques to search for nanocrystals within clusters of calcium phosphate sequestered by bovine phosphopeptides, derived from osteopontin or casein. The neutron diffraction patterns show broad features that could be consistent with hexagonal hydroxyapatite crystallites smaller than 18.9 Å. Such nanocrystallites are, however, undetected by the complementary X-ray and FTIR data, collected on the same samples. The absence of a distinct diffraction pattern from the nanoclusters supports the generally accepted amorphous calcium phosphate structure of the mineral core. (Less)
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published
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in
European biophysics journal : EBJ
publisher
Springer
external identifiers
  • pmid:26780236
  • scopus:84954456210
  • wos:000378049100003
ISSN
1432-1017
DOI
10.1007/s00249-015-1109-7
language
English
LU publication?
yes
id
2efc164a-d529-4351-babb-f1291afbb254 (old id 8577137)
date added to LUP
2016-02-10 14:11:06
date last changed
2017-05-21 03:53:10
@article{2efc164a-d529-4351-babb-f1291afbb254,
  abstract     = {There are abundant examples of nanoclusters and inorganic microcrystals in biology. Their study under physiologically relevant conditions remains challenging due to their heterogeneity, instability, and the requirements of sample preparation. Advantages of using neutron diffraction and contrast matching to characterize biomaterials are highlighted in this article. We have applied these and complementary techniques to search for nanocrystals within clusters of calcium phosphate sequestered by bovine phosphopeptides, derived from osteopontin or casein. The neutron diffraction patterns show broad features that could be consistent with hexagonal hydroxyapatite crystallites smaller than 18.9 Å. Such nanocrystallites are, however, undetected by the complementary X-ray and FTIR data, collected on the same samples. The absence of a distinct diffraction pattern from the nanoclusters supports the generally accepted amorphous calcium phosphate structure of the mineral core.},
  author       = {Lenton, Samuel and Nylander, Tommy and Holt, Carl and Sawyer, Lindsay and Härtlein, Michael and Müller, Harrald and Teixeira, Susana C M},
  issn         = {1432-1017},
  language     = {eng},
  month        = {01},
  publisher    = {Springer},
  series       = {European biophysics journal : EBJ},
  title        = {Structural studies of hydrated samples of amorphous calcium phosphate and phosphoprotein nanoclusters.},
  url          = {http://dx.doi.org/10.1007/s00249-015-1109-7},
  year         = {2016},
}