Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells
(2022) In Scandinavian Journal of Immunology 96(5). p.1-12- Abstract
The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103+CD11b+ cDC in the murine small intestine (SI). GP2 expression was induced on CD103+CD11b+ cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103+CD11b+ cDC (huLang-Cre.gp2fl/fl mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2... (More)
The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103+CD11b+ cDC in the murine small intestine (SI). GP2 expression was induced on CD103+CD11b+ cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103+CD11b+ cDC (huLang-Cre.gp2fl/fl mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2 expression by CD103+CD11b+ cDC is not required for intestinal immune homoeostasis.
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- author
- Luda, Katarzyna M. LU ; Da Silva, Clement LU ; Ahmadi, Fatemeh LU ; Mowat, Allan Mcl LU ; Ohno, Hiroshi ; Kotarsky, Knut LU and Agace, William W. LU
- organization
- publishing date
- 2022-11
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cDC2, dendritic cell, homeostasis, intestine, IRF4, retinoic acid, TGFβR1
- in
- Scandinavian Journal of Immunology
- volume
- 96
- issue
- 5
- article number
- e13219
- pages
- 1 - 12
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:85140009640
- ISSN
- 0300-9475
- DOI
- 10.1111/sji.13219
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2022 The Authors. Scandinavian Journal of Immunology published by John Wiley & Sons Ltd on behalf of The Scandinavian Foundation for Immunology.
- id
- 859dfe8c-562a-45e0-9102-99b10ce3e3f4
- date added to LUP
- 2022-11-18 16:10:02
- date last changed
- 2023-04-06 00:41:09
@article{859dfe8c-562a-45e0-9102-99b10ce3e3f4, abstract = {{<p>The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103<sup>+</sup>CD11b<sup>+</sup> cDC in the murine small intestine (SI). GP2 expression was induced on CD103<sup>+</sup>CD11b<sup>+</sup> cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103<sup>+</sup>CD11b<sup>+</sup> cDC (huLang-Cre.gp2<sup>fl/fl</sup> mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2 expression by CD103<sup>+</sup>CD11b<sup>+</sup> cDC is not required for intestinal immune homoeostasis.</p>}}, author = {{Luda, Katarzyna M. and Da Silva, Clement and Ahmadi, Fatemeh and Mowat, Allan Mcl and Ohno, Hiroshi and Kotarsky, Knut and Agace, William W.}}, issn = {{0300-9475}}, keywords = {{cDC2; dendritic cell; homeostasis; intestine; IRF4; retinoic acid; TGFβR1}}, language = {{eng}}, number = {{5}}, pages = {{1--12}}, publisher = {{Wiley-Blackwell}}, series = {{Scandinavian Journal of Immunology}}, title = {{Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells}}, url = {{http://dx.doi.org/10.1111/sji.13219}}, doi = {{10.1111/sji.13219}}, volume = {{96}}, year = {{2022}}, }