Evaluation of transcriptional biomarkers using a high-resolution regression approach : Concentration-dependence of selected transcripts in copper-exposed freshwater mussels (Anodonta anatina)
(2022) In Environmental Toxicology and Pharmacology 90.- Abstract
We tested concentration-dependence of selected gene transcripts (cat, gst, hsp70, hsp90, mt and sod) for evaluation as biomarkers of chemical stress. Contrary to the common approach of factorial designs and few exposure concentrations, we used regression across a high-resolution concentration series. Specifically, freshwater mussels (Anodonta anatina) were acutely (96 h) exposed to Cu (13 nominal concentrations, measuring 0.13-1 600 µg/L), and transcripts were measured by RT-qPCR. In digestive glands, cat, hsp90 and mt decreased with water Cu (p < 0.05), but response magnitudes saturated at < 2-fold decreases. In gills, gst, hsp70, hsp90 and mt increased with water Cu (p < 0.05). While hsp70, hsp90 and mt exceeded 2-fold... (More)
We tested concentration-dependence of selected gene transcripts (cat, gst, hsp70, hsp90, mt and sod) for evaluation as biomarkers of chemical stress. Contrary to the common approach of factorial designs and few exposure concentrations, we used regression across a high-resolution concentration series. Specifically, freshwater mussels (Anodonta anatina) were acutely (96 h) exposed to Cu (13 nominal concentrations, measuring 0.13-1 600 µg/L), and transcripts were measured by RT-qPCR. In digestive glands, cat, hsp90 and mt decreased with water Cu (p < 0.05), but response magnitudes saturated at < 2-fold decreases. In gills, gst, hsp70, hsp90 and mt increased with water Cu (p < 0.05). While hsp70, hsp90 and mt exceeded 2-fold increases within the exposure range, high Cu concentrations were required (38-160 µg/L). Although gill responses were generally more robust compared to digestive glands, overall small response magnitudes and moderate sensitivity may set limit for potential application as general biomarkers of chemical stress.
(Less)
- author
- Ekelund Ugge, Gustaf M O LU ; Jonsson, Annie ; Walstad, Anders and Berglund, Olof LU
- organization
- publishing date
- 2022-02
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Environmental Toxicology and Pharmacology
- volume
- 90
- article number
- 103795
- pages
- 11 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:34971800
- scopus:85122497764
- ISSN
- 1872-7077
- DOI
- 10.1016/j.etap.2021.103795
- project
- Transcriptional biomarkers of toxicity - powerful tools or random noise? An applied perspective from studies on bivalves
- language
- English
- LU publication?
- yes
- id
- 85bf4255-b9ca-4f6a-b21e-1e98f21f4c37
- date added to LUP
- 2022-01-26 08:02:46
- date last changed
- 2024-09-22 09:26:37
@article{85bf4255-b9ca-4f6a-b21e-1e98f21f4c37, abstract = {{<p>We tested concentration-dependence of selected gene transcripts (cat, gst, hsp70, hsp90, mt and sod) for evaluation as biomarkers of chemical stress. Contrary to the common approach of factorial designs and few exposure concentrations, we used regression across a high-resolution concentration series. Specifically, freshwater mussels (Anodonta anatina) were acutely (96 h) exposed to Cu (13 nominal concentrations, measuring 0.13-1 600 µg/L), and transcripts were measured by RT-qPCR. In digestive glands, cat, hsp90 and mt decreased with water Cu (p < 0.05), but response magnitudes saturated at < 2-fold decreases. In gills, gst, hsp70, hsp90 and mt increased with water Cu (p < 0.05). While hsp70, hsp90 and mt exceeded 2-fold increases within the exposure range, high Cu concentrations were required (38-160 µg/L). Although gill responses were generally more robust compared to digestive glands, overall small response magnitudes and moderate sensitivity may set limit for potential application as general biomarkers of chemical stress.</p>}}, author = {{Ekelund Ugge, Gustaf M O and Jonsson, Annie and Walstad, Anders and Berglund, Olof}}, issn = {{1872-7077}}, language = {{eng}}, publisher = {{Elsevier}}, series = {{Environmental Toxicology and Pharmacology}}, title = {{Evaluation of transcriptional biomarkers using a high-resolution regression approach : Concentration-dependence of selected transcripts in copper-exposed freshwater mussels (Anodonta anatina)}}, url = {{http://dx.doi.org/10.1016/j.etap.2021.103795}}, doi = {{10.1016/j.etap.2021.103795}}, volume = {{90}}, year = {{2022}}, }