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Evaluation of transcriptional biomarkers using a high-resolution regression approach : Concentration-dependence of selected transcripts in copper-exposed freshwater mussels (Anodonta anatina)

Ekelund Ugge, Gustaf M O LU orcid ; Jonsson, Annie ; Walstad, Anders and Berglund, Olof LU (2022) In Environmental Toxicology and Pharmacology 90.
Abstract

We tested concentration-dependence of selected gene transcripts (cat, gst, hsp70, hsp90, mt and sod) for evaluation as biomarkers of chemical stress. Contrary to the common approach of factorial designs and few exposure concentrations, we used regression across a high-resolution concentration series. Specifically, freshwater mussels (Anodonta anatina) were acutely (96 h) exposed to Cu (13 nominal concentrations, measuring 0.13-1 600 µg/L), and transcripts were measured by RT-qPCR. In digestive glands, cat, hsp90 and mt decreased with water Cu (p < 0.05), but response magnitudes saturated at < 2-fold decreases. In gills, gst, hsp70, hsp90 and mt increased with water Cu (p < 0.05). While hsp70, hsp90 and mt exceeded 2-fold... (More)

We tested concentration-dependence of selected gene transcripts (cat, gst, hsp70, hsp90, mt and sod) for evaluation as biomarkers of chemical stress. Contrary to the common approach of factorial designs and few exposure concentrations, we used regression across a high-resolution concentration series. Specifically, freshwater mussels (Anodonta anatina) were acutely (96 h) exposed to Cu (13 nominal concentrations, measuring 0.13-1 600 µg/L), and transcripts were measured by RT-qPCR. In digestive glands, cat, hsp90 and mt decreased with water Cu (p < 0.05), but response magnitudes saturated at < 2-fold decreases. In gills, gst, hsp70, hsp90 and mt increased with water Cu (p < 0.05). While hsp70, hsp90 and mt exceeded 2-fold increases within the exposure range, high Cu concentrations were required (38-160 µg/L). Although gill responses were generally more robust compared to digestive glands, overall small response magnitudes and moderate sensitivity may set limit for potential application as general biomarkers of chemical stress.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Environmental Toxicology and Pharmacology
volume
90
article number
103795
pages
11 pages
publisher
Elsevier
external identifiers
  • pmid:34971800
  • scopus:85122497764
ISSN
1872-7077
DOI
10.1016/j.etap.2021.103795
project
Transcriptional biomarkers of toxicity - powerful tools or random noise? An applied perspective from studies on bivalves
language
English
LU publication?
yes
id
85bf4255-b9ca-4f6a-b21e-1e98f21f4c37
date added to LUP
2022-01-26 08:02:46
date last changed
2024-06-16 00:28:35
@article{85bf4255-b9ca-4f6a-b21e-1e98f21f4c37,
  abstract     = {{<p>We tested concentration-dependence of selected gene transcripts (cat, gst, hsp70, hsp90, mt and sod) for evaluation as biomarkers of chemical stress. Contrary to the common approach of factorial designs and few exposure concentrations, we used regression across a high-resolution concentration series. Specifically, freshwater mussels (Anodonta anatina) were acutely (96 h) exposed to Cu (13 nominal concentrations, measuring 0.13-1 600 µg/L), and transcripts were measured by RT-qPCR. In digestive glands, cat, hsp90 and mt decreased with water Cu (p &lt; 0.05), but response magnitudes saturated at &lt; 2-fold decreases. In gills, gst, hsp70, hsp90 and mt increased with water Cu (p &lt; 0.05). While hsp70, hsp90 and mt exceeded 2-fold increases within the exposure range, high Cu concentrations were required (38-160 µg/L). Although gill responses were generally more robust compared to digestive glands, overall small response magnitudes and moderate sensitivity may set limit for potential application as general biomarkers of chemical stress.</p>}},
  author       = {{Ekelund Ugge, Gustaf M O and Jonsson, Annie and Walstad, Anders and Berglund, Olof}},
  issn         = {{1872-7077}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Environmental Toxicology and Pharmacology}},
  title        = {{Evaluation of transcriptional biomarkers using a high-resolution regression approach : Concentration-dependence of selected transcripts in copper-exposed freshwater mussels (Anodonta anatina)}},
  url          = {{http://dx.doi.org/10.1016/j.etap.2021.103795}},
  doi          = {{10.1016/j.etap.2021.103795}},
  volume       = {{90}},
  year         = {{2022}},
}