Mucosa-associated bacteria in two middle-aged women diagnosed with collagenous colitis.
(2012) In World Journal of Gastroenterology 18(14). p.1628-1634- Abstract
- AIM:
To characterize the colon microbiota in two women histologically diagnosed with collagenous colitis using a culture-independent method.
METHODS:
Biopsies were taken from the ascending colon and the total DNA was extracted. Universal bacterial primers were used to amplify the bacterial 16S rRNA genes. The amplicons were then cloned into competent Escherichia coli cells. The clones were sequenced and identified by comparison to known sequences.
RESULTS:
The clones could be divided into 44 different phylotypes. The microbiota was dominated by Firmicutes and Bacteroidetes. Seven phylotypes were found in both patients and constituted 47.5% of the total number of clones. Of... (More) - AIM:
To characterize the colon microbiota in two women histologically diagnosed with collagenous colitis using a culture-independent method.
METHODS:
Biopsies were taken from the ascending colon and the total DNA was extracted. Universal bacterial primers were used to amplify the bacterial 16S rRNA genes. The amplicons were then cloned into competent Escherichia coli cells. The clones were sequenced and identified by comparison to known sequences.
RESULTS:
The clones could be divided into 44 different phylotypes. The microbiota was dominated by Firmicutes and Bacteroidetes. Seven phylotypes were found in both patients and constituted 47.5% of the total number of clones. Of these, the most dominating were clones similar to Bacteroides cellulosilyticus, Bacteroides caccae, Bacteroides thetaiotaomicron, Bacteroides uniformis and Bacteroides dorei within Bacteroidetes. Sequences similar to Faecalibacterium prausnitzii and Clostridium citroniae were also found in both patients.
CONCLUSION:
A predominance of potentially pathogenic Bacteroides spp., and the presence of clones showing similarity to Clostridium clostridioforme were found but the overall colon microbiota showed similarities to a healthy one. Etiologies for collagenous colitis other than an adverse bacterial flora must also be considered. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2519133
- author
- Gustafsson, Rita LU ; Ohlsson, Bodil LU ; Benoni, Cecilia LU ; Jeppsson, Bengt LU and Olsson, Crister LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- World Journal of Gastroenterology
- volume
- 18
- issue
- 14
- pages
- 1628 - 1634
- publisher
- WJG Press
- external identifiers
-
- wos:000302931000010
- pmid:22529692
- scopus:84859962671
- pmid:22529692
- ISSN
- 1007-9327
- DOI
- 10.3748/wjg.v18.i14.1628
- language
- English
- LU publication?
- yes
- id
- 85c27370-26bd-40af-a5df-83279da729a6 (old id 2519133)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22529692?dopt=Abstract
- date added to LUP
- 2016-04-04 07:40:41
- date last changed
- 2022-01-29 02:27:27
@article{85c27370-26bd-40af-a5df-83279da729a6,
abstract = {{AIM:<br/><br>
To characterize the colon microbiota in two women histologically diagnosed with collagenous colitis using a culture-independent method.<br/><br>
<br/><br>
METHODS:<br/><br>
Biopsies were taken from the ascending colon and the total DNA was extracted. Universal bacterial primers were used to amplify the bacterial 16S rRNA genes. The amplicons were then cloned into competent Escherichia coli cells. The clones were sequenced and identified by comparison to known sequences.<br/><br>
<br/><br>
RESULTS:<br/><br>
The clones could be divided into 44 different phylotypes. The microbiota was dominated by Firmicutes and Bacteroidetes. Seven phylotypes were found in both patients and constituted 47.5% of the total number of clones. Of these, the most dominating were clones similar to Bacteroides cellulosilyticus, Bacteroides caccae, Bacteroides thetaiotaomicron, Bacteroides uniformis and Bacteroides dorei within Bacteroidetes. Sequences similar to Faecalibacterium prausnitzii and Clostridium citroniae were also found in both patients.<br/><br>
<br/><br>
CONCLUSION:<br/><br>
A predominance of potentially pathogenic Bacteroides spp., and the presence of clones showing similarity to Clostridium clostridioforme were found but the overall colon microbiota showed similarities to a healthy one. Etiologies for collagenous colitis other than an adverse bacterial flora must also be considered.}},
author = {{Gustafsson, Rita and Ohlsson, Bodil and Benoni, Cecilia and Jeppsson, Bengt and Olsson, Crister}},
issn = {{1007-9327}},
language = {{eng}},
number = {{14}},
pages = {{1628--1634}},
publisher = {{WJG Press}},
series = {{World Journal of Gastroenterology}},
title = {{Mucosa-associated bacteria in two middle-aged women diagnosed with collagenous colitis.}},
url = {{http://dx.doi.org/10.3748/wjg.v18.i14.1628}},
doi = {{10.3748/wjg.v18.i14.1628}},
volume = {{18}},
year = {{2012}},
}