Evaluation of a Probing Feeding Strategy in Large Scale Cultivations
(2002) In Technical Reports TFRT-7601- Abstract
- This report presents the results of two \emph{Escherichia~coli} cultivations performed in a large scale industrial bioreactor. The experiments aimed at evaluating a probing feeding strategy that adresses the problem of acetate accumulation. The principle is to superimpose short pulses in the glucose feed and evaluate the response in the dissolved oxygen signal for the feed adjustments. Simulations of a two compartments system are also done to give insight into the possible effects of the scale and the imperfect mixing on the feeding strategy. Satisfactory results in terms of recombinant protein amounts have been obtained and no major adverse large scale effects were observed.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8602652
- author
- Velut, Stéphane ; de Maré, Lena LU ; Axelsson, Jan Peter and Hagander, Per LU
- organization
- publishing date
- 2002
- type
- Book/Report
- publication status
- published
- subject
- in
- Technical Reports TFRT-7601
- publisher
- Department of Automatic Control, Lund Institute of Technology (LTH)
- ISSN
- 0280-5316
- language
- English
- LU publication?
- yes
- id
- 435c70c0-fcd8-498c-a018-50a55e8ae6ee (old id 8602652)
- date added to LUP
- 2016-04-01 15:37:21
- date last changed
- 2018-11-21 20:35:26
@techreport{435c70c0-fcd8-498c-a018-50a55e8ae6ee, abstract = {{This report presents the results of two \emph{Escherichia~coli} cultivations performed in a large scale industrial bioreactor. The experiments aimed at evaluating a probing feeding strategy that adresses the problem of acetate accumulation. The principle is to superimpose short pulses in the glucose feed and evaluate the response in the dissolved oxygen signal for the feed adjustments. Simulations of a two compartments system are also done to give insight into the possible effects of the scale and the imperfect mixing on the feeding strategy. Satisfactory results in terms of recombinant protein amounts have been obtained and no major adverse large scale effects were observed.}}, author = {{Velut, Stéphane and de Maré, Lena and Axelsson, Jan Peter and Hagander, Per}}, institution = {{Department of Automatic Control, Lund Institute of Technology (LTH)}}, issn = {{0280-5316}}, language = {{eng}}, series = {{Technical Reports TFRT-7601}}, title = {{Evaluation of a Probing Feeding Strategy in Large Scale Cultivations}}, url = {{https://lup.lub.lu.se/search/files/4434402/8627792.pdf}}, year = {{2002}}, }