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Evaluation of a probing feeding strategy in large scale cultivations

Axelsson, Jan Peter; de Maré, Lena LU ; Hagander, Per LU and Velut, Stéphane (2002) In Report TFRT 7601.
Abstract
This report presents the results of two \emph{Escherichia~coli} cultivations performed in a large scale industrial bioreactor. The experiments aimed at evaluating a probing feeding strategy that adresses the problem of acetate accumulation. The principle is to superimpose short pulses in the glucose feed and evaluate the response in the dissolved oxygen signal for the feed adjustments. Simulations of a two compartments system are also done to give insight into the possible effects of the scale and the imperfect mixing on the feeding strategy. Satisfactory results in terms of recombinant protein amounts have been obtained and no major adverse large scale effects were observed.
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author
organization
publishing date
type
Book/Report
publication status
published
subject
in
Report TFRT
volume
7601
publisher
Department of Automatic Control, Lund Institute of Technology (LTH)
ISSN
0280-5316
language
English
LU publication?
yes
id
435c70c0-fcd8-498c-a018-50a55e8ae6ee (old id 8602652)
date added to LUP
2016-02-15 19:09:50
date last changed
2016-04-16 03:09:36
@techreport{435c70c0-fcd8-498c-a018-50a55e8ae6ee,
  abstract     = {This report presents the results of two \emph{Escherichia~coli} cultivations performed in a large scale industrial bioreactor. The experiments aimed at evaluating a probing feeding strategy that adresses the problem of acetate accumulation. The principle is to superimpose short pulses in the glucose feed and evaluate the response in the dissolved oxygen signal for the feed adjustments. Simulations of a two compartments system are also done to give insight into the possible effects of the scale and the imperfect mixing on the feeding strategy. Satisfactory results in terms of recombinant protein amounts have been obtained and no major adverse large scale effects were observed.},
  author       = {Axelsson, Jan Peter and de Maré, Lena and Hagander, Per and Velut, Stéphane},
  institution  = {Department of Automatic Control, Lund Institute of Technology (LTH)},
  issn         = {0280-5316},
  language     = {eng},
  series       = {Report TFRT},
  title        = {Evaluation of a probing feeding strategy in large scale cultivations},
  volume       = {7601},
  year         = {2002},
}