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Regulation of vascular smooth muscle mechanotransduction by microRNAs and L-type calcium channels.

Turczynska, Karolina LU ; Hellstrand, Per LU ; Swärd, Karl LU and Albinsson, Sebastian LU (2013) In Communicative & Integrative Biology 6(1). p.22278-22278
Abstract
The phenotype of smooth muscle cells is regulated by multiple environmental factors including mechanical forces. Mechanical stretch of mouse portal veins ex vivo has been shown to promote contractile differentiation by activation of the Rho-pathway, an effect that is dependent on the influx of calcium via L-type calcium channels. MicroRNAs have recently been demonstrated to play a significant role in the control of smooth muscle phenotype and in a recent report we investigated their role in vascular mechanosensing. By smooth muscle specific deletion of Dicer, we found that microRNAs are essential for smooth muscle differentiation in response to stretch by regulating CamKIIδ and L-type calcium channel expression. Furthermore, we suggest... (More)
The phenotype of smooth muscle cells is regulated by multiple environmental factors including mechanical forces. Mechanical stretch of mouse portal veins ex vivo has been shown to promote contractile differentiation by activation of the Rho-pathway, an effect that is dependent on the influx of calcium via L-type calcium channels. MicroRNAs have recently been demonstrated to play a significant role in the control of smooth muscle phenotype and in a recent report we investigated their role in vascular mechanosensing. By smooth muscle specific deletion of Dicer, we found that microRNAs are essential for smooth muscle differentiation in response to stretch by regulating CamKIIδ and L-type calcium channel expression. Furthermore, we suggest that loss of L-type calcium channels in Dicer KO is due to reduced expression of the smooth muscle-enriched microRNA, miR-145, which targets CamKIIδ. These results unveil a novel mechanism for miR-145 dependent regulation of smooth muscle phenotype. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Communicative & Integrative Biology
volume
6
issue
1
pages
22278 - 22278
publisher
Landes Bioscience
external identifiers
  • pmid:23802033
  • scopus:84875089318
ISSN
1942-0889
DOI
10.4161/cib.22278
language
English
LU publication?
yes
id
86087cff-0f1e-420d-85f5-cae80fa47a15 (old id 3912971)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23802033?dopt=Abstract
date added to LUP
2013-07-01 15:43:35
date last changed
2019-04-10 02:29:23
@article{86087cff-0f1e-420d-85f5-cae80fa47a15,
  abstract     = {The phenotype of smooth muscle cells is regulated by multiple environmental factors including mechanical forces. Mechanical stretch of mouse portal veins ex vivo has been shown to promote contractile differentiation by activation of the Rho-pathway, an effect that is dependent on the influx of calcium via L-type calcium channels. MicroRNAs have recently been demonstrated to play a significant role in the control of smooth muscle phenotype and in a recent report we investigated their role in vascular mechanosensing. By smooth muscle specific deletion of Dicer, we found that microRNAs are essential for smooth muscle differentiation in response to stretch by regulating CamKIIδ and L-type calcium channel expression. Furthermore, we suggest that loss of L-type calcium channels in Dicer KO is due to reduced expression of the smooth muscle-enriched microRNA, miR-145, which targets CamKIIδ. These results unveil a novel mechanism for miR-145 dependent regulation of smooth muscle phenotype.},
  author       = {Turczynska, Karolina and Hellstrand, Per and Swärd, Karl and Albinsson, Sebastian},
  issn         = {1942-0889},
  language     = {eng},
  number       = {1},
  pages        = {22278--22278},
  publisher    = {Landes Bioscience},
  series       = {Communicative & Integrative Biology},
  title        = {Regulation of vascular smooth muscle mechanotransduction by microRNAs and L-type calcium channels.},
  url          = {http://dx.doi.org/10.4161/cib.22278},
  volume       = {6},
  year         = {2013},
}