Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Antibodies directed against the pancreatic islet cell plasma membrane detection and specificity

Lernmark, Å LU orcid ; Kanatsuna, T. ; Patzelt, C. ; Diakoumis, K. ; Carroll, R. ; Rubenstein, A. H. and Steiner, D. F. (1980) In Diabetologia 19(5). p.445-451
Abstract


Rabbits were immunised with suspensions of viable, insulin-producing islet cells prepared from collagenase-isolated rat or ob/ob mouse pancreatic islets. Antibodies reactive with the surface of dispersed rat islet cells were present in both the rabbit anti-rat and the rabbit anti -ob/ob mouse islet sera as revealed by indirect immunofluorescence or by a radioligandassay using
125
I-Protein A as a measure of cell bound IgG. In a competition assay the binding of
125
I-Protein A was displaced in a concentration dependent manner by non-radioactive Protein A.... (More)


Rabbits were immunised with suspensions of viable, insulin-producing islet cells prepared from collagenase-isolated rat or ob/ob mouse pancreatic islets. Antibodies reactive with the surface of dispersed rat islet cells were present in both the rabbit anti-rat and the rabbit anti -ob/ob mouse islet sera as revealed by indirect immunofluorescence or by a radioligandassay using
125
I-Protein A as a measure of cell bound IgG. In a competition assay the binding of
125
I-Protein A was displaced in a concentration dependent manner by non-radioactive Protein A. Maximal displacement was found at concentrations of Protein A higher than 0.1 μg. added to 10
5
islet cells. Although not always detected by immunofluorescence there was a several-fold increase above normal rabbit serum of
125
I-Protein A-binding to rat hepatocytes and spleen lymphocytes incubated with the islet cell antisera. Conversely, rabbit antisera against rat spleen lymphocytes or against a rat liver plasma membrane preparation reacted with rat islet cells. The rabbit anti-rat islet cell antiserum was absorbed to both spleen lymphocytes and hepatocytes until there was no binding of
125
I-Protein A to either cell type. Islet specific antibodies were still present since this doubly absorbed antiserum induced cell surface immunofluorescence as well as
125
I-Protein A-binding to rat islet cells. It is concluded that apart from common antigenic determinants immunisation with viable islet cells induces formation of antibodies directed against specific islet cell surface components.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
autoimmunity, cell surface antigens, cell surface immunofluorescence, cell suspensions, diabetes mellitus, islet cell surface antibodies, Pancreatic islet cells, Protein A radioassay
in
Diabetologia
volume
19
issue
5
pages
445 - 451
publisher
Springer
external identifiers
  • scopus:0019227063
  • pmid:7004963
ISSN
0012-186X
DOI
10.1007/BF00281824
language
English
LU publication?
no
id
864832c6-42f7-41e3-920d-c7acf1602bba
date added to LUP
2019-09-16 15:37:41
date last changed
2025-10-14 09:29:07
@article{864832c6-42f7-41e3-920d-c7acf1602bba,
  abstract     = {{<p><br>
                            Rabbits were immunised with suspensions of viable, insulin-producing islet cells prepared from collagenase-isolated rat or ob/ob mouse pancreatic islets. Antibodies reactive with the surface of dispersed rat islet cells were present in both the rabbit anti-rat and the rabbit anti -ob/ob mouse islet sera as revealed by indirect immunofluorescence or by a radioligandassay using <br>
                            <sup>125</sup><br>
                            I-Protein A as a measure of cell bound IgG. In a competition assay the binding of <br>
                            <sup>125</sup><br>
                            I-Protein A was displaced in a concentration dependent manner by non-radioactive Protein A. Maximal displacement was found at concentrations of Protein A higher than 0.1 μg. added to 10<br>
                            <sup>5</sup><br>
                             islet cells. Although not always detected by immunofluorescence there was a several-fold increase above normal rabbit serum of <br>
                            <sup>125</sup><br>
                            I-Protein A-binding to rat hepatocytes and spleen lymphocytes incubated with the islet cell antisera. Conversely, rabbit antisera against rat spleen lymphocytes or against a rat liver plasma membrane preparation reacted with rat islet cells. The rabbit anti-rat islet cell antiserum was absorbed to both spleen lymphocytes and hepatocytes until there was no binding of <br>
                            <sup>125</sup><br>
                            I-Protein A to either cell type. Islet specific antibodies were still present since this doubly absorbed antiserum induced cell surface immunofluorescence as well as <br>
                            <sup>125</sup><br>
                            I-Protein A-binding to rat islet cells. It is concluded that apart from common antigenic determinants immunisation with viable islet cells induces formation of antibodies directed against specific islet cell surface components.</p>}},
  author       = {{Lernmark, Å and Kanatsuna, T. and Patzelt, C. and Diakoumis, K. and Carroll, R. and Rubenstein, A. H. and Steiner, D. F.}},
  issn         = {{0012-186X}},
  keywords     = {{autoimmunity; cell surface antigens; cell surface immunofluorescence; cell suspensions; diabetes mellitus; islet cell surface antibodies; Pancreatic islet cells; Protein A radioassay}},
  language     = {{eng}},
  month        = {{11}},
  number       = {{5}},
  pages        = {{445--451}},
  publisher    = {{Springer}},
  series       = {{Diabetologia}},
  title        = {{Antibodies directed against the pancreatic islet cell plasma membrane detection and specificity}},
  url          = {{http://dx.doi.org/10.1007/BF00281824}},
  doi          = {{10.1007/BF00281824}},
  volume       = {{19}},
  year         = {{1980}},
}