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Antibodies directed against the pancreatic islet cell plasma membrane detection and specificity

Lernmark, Å LU orcid ; Kanatsuna, T. ; Patzelt, C. ; Diakoumis, K. ; Carroll, R. ; Rubenstein, A. H. and Steiner, D. F. (1980) In Diabetologia 19(5). p.445-451
Abstract


Rabbits were immunised with suspensions of viable, insulin-producing islet cells prepared from collagenase-isolated rat or ob/ob mouse pancreatic islets. Antibodies reactive with the surface of dispersed rat islet cells were present in both the rabbit anti-rat and the rabbit anti -ob/ob mouse islet sera as revealed by indirect immunofluorescence or by a radioligandassay using
125
I-Protein A as a measure of cell bound IgG. In a competition assay the binding of
125
I-Protein A was displaced in a concentration dependent manner by non-radioactive Protein A.... (More)


Rabbits were immunised with suspensions of viable, insulin-producing islet cells prepared from collagenase-isolated rat or ob/ob mouse pancreatic islets. Antibodies reactive with the surface of dispersed rat islet cells were present in both the rabbit anti-rat and the rabbit anti -ob/ob mouse islet sera as revealed by indirect immunofluorescence or by a radioligandassay using
125
I-Protein A as a measure of cell bound IgG. In a competition assay the binding of
125
I-Protein A was displaced in a concentration dependent manner by non-radioactive Protein A. Maximal displacement was found at concentrations of Protein A higher than 0.1 μg. added to 10
5
islet cells. Although not always detected by immunofluorescence there was a several-fold increase above normal rabbit serum of
125
I-Protein A-binding to rat hepatocytes and spleen lymphocytes incubated with the islet cell antisera. Conversely, rabbit antisera against rat spleen lymphocytes or against a rat liver plasma membrane preparation reacted with rat islet cells. The rabbit anti-rat islet cell antiserum was absorbed to both spleen lymphocytes and hepatocytes until there was no binding of
125
I-Protein A to either cell type. Islet specific antibodies were still present since this doubly absorbed antiserum induced cell surface immunofluorescence as well as
125
I-Protein A-binding to rat islet cells. It is concluded that apart from common antigenic determinants immunisation with viable islet cells induces formation of antibodies directed against specific islet cell surface components.

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
autoimmunity, cell surface antigens, cell surface immunofluorescence, cell suspensions, diabetes mellitus, islet cell surface antibodies, Pancreatic islet cells, Protein A radioassay
in
Diabetologia
volume
19
issue
5
pages
445 - 451
publisher
Springer
external identifiers
  • pmid:7004963
  • scopus:0019227063
ISSN
0012-186X
DOI
10.1007/BF00281824
language
English
LU publication?
no
id
864832c6-42f7-41e3-920d-c7acf1602bba
date added to LUP
2019-09-16 15:37:41
date last changed
2024-03-13 08:06:29
@article{864832c6-42f7-41e3-920d-c7acf1602bba,
  abstract     = {{<p><br>
                            Rabbits were immunised with suspensions of viable, insulin-producing islet cells prepared from collagenase-isolated rat or ob/ob mouse pancreatic islets. Antibodies reactive with the surface of dispersed rat islet cells were present in both the rabbit anti-rat and the rabbit anti -ob/ob mouse islet sera as revealed by indirect immunofluorescence or by a radioligandassay using <br>
                            <sup>125</sup><br>
                            I-Protein A as a measure of cell bound IgG. In a competition assay the binding of <br>
                            <sup>125</sup><br>
                            I-Protein A was displaced in a concentration dependent manner by non-radioactive Protein A. Maximal displacement was found at concentrations of Protein A higher than 0.1 μg. added to 10<br>
                            <sup>5</sup><br>
                             islet cells. Although not always detected by immunofluorescence there was a several-fold increase above normal rabbit serum of <br>
                            <sup>125</sup><br>
                            I-Protein A-binding to rat hepatocytes and spleen lymphocytes incubated with the islet cell antisera. Conversely, rabbit antisera against rat spleen lymphocytes or against a rat liver plasma membrane preparation reacted with rat islet cells. The rabbit anti-rat islet cell antiserum was absorbed to both spleen lymphocytes and hepatocytes until there was no binding of <br>
                            <sup>125</sup><br>
                            I-Protein A to either cell type. Islet specific antibodies were still present since this doubly absorbed antiserum induced cell surface immunofluorescence as well as <br>
                            <sup>125</sup><br>
                            I-Protein A-binding to rat islet cells. It is concluded that apart from common antigenic determinants immunisation with viable islet cells induces formation of antibodies directed against specific islet cell surface components.</p>}},
  author       = {{Lernmark, Å and Kanatsuna, T. and Patzelt, C. and Diakoumis, K. and Carroll, R. and Rubenstein, A. H. and Steiner, D. F.}},
  issn         = {{0012-186X}},
  keywords     = {{autoimmunity; cell surface antigens; cell surface immunofluorescence; cell suspensions; diabetes mellitus; islet cell surface antibodies; Pancreatic islet cells; Protein A radioassay}},
  language     = {{eng}},
  month        = {{11}},
  number       = {{5}},
  pages        = {{445--451}},
  publisher    = {{Springer}},
  series       = {{Diabetologia}},
  title        = {{Antibodies directed against the pancreatic islet cell plasma membrane detection and specificity}},
  url          = {{http://dx.doi.org/10.1007/BF00281824}},
  doi          = {{10.1007/BF00281824}},
  volume       = {{19}},
  year         = {{1980}},
}