Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosine.
(2012) In FEMS Microbiology Letters 331(2). p.120-127- Abstract
- Deoxyribonucleoside kinases (dNKs) are essential in the mammalian cell but their 'importance' in bacteria, especially aquatic ones, is less clear. We studied two aquatic bacteria, Gram-negative Flavobacterium psychrophilum JIP02/86 and Polaribacter sp. MED152, for their ability to salvage deoxyribonucleosides (dNs). Both had a Gram-positive-type thymidine kinase (TK1), which could phosphorylate thymidine, and one non-TK1 dNK, which could efficiently phosphorylate deoxyadenosine and slightly also deoxycytosine. Surprisingly, the four tested dNKs could not phosphorylate deoxyguanosine, and apparently, these two bacteria are missing this activity. When tens of available aquatic bacteria genomes were examined for the presence of dNKs, a... (More)
- Deoxyribonucleoside kinases (dNKs) are essential in the mammalian cell but their 'importance' in bacteria, especially aquatic ones, is less clear. We studied two aquatic bacteria, Gram-negative Flavobacterium psychrophilum JIP02/86 and Polaribacter sp. MED152, for their ability to salvage deoxyribonucleosides (dNs). Both had a Gram-positive-type thymidine kinase (TK1), which could phosphorylate thymidine, and one non-TK1 dNK, which could efficiently phosphorylate deoxyadenosine and slightly also deoxycytosine. Surprisingly, the four tested dNKs could not phosphorylate deoxyguanosine, and apparently, these two bacteria are missing this activity. When tens of available aquatic bacteria genomes were examined for the presence of dNKs, a majority had at least a TK1-like gene, but several lacked any dNKs. Apparently, among aquatic bacteria, the role of the dN salvage varies. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2520042
- author
- Tinta, Tinkara LU ; Slot Christiansen, Louise LU ; Konrad, Anke ; Liberles, David A ; Turk, Valentina ; Munch-Petersen, Birgitte ; Piskur, Jure LU and Clausen, Anders Ranegaard LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- aquatic bacteria, 3H-thymidine incorporation, deoxyribonucleoside kinase, nucleoside salvage
- in
- FEMS Microbiology Letters
- volume
- 331
- issue
- 2
- pages
- 120 - 127
- publisher
- Oxford University Press
- external identifiers
-
- wos:000304187800005
- pmid:22462611
- scopus:84861233766
- pmid:22462611
- ISSN
- 1574-6968
- DOI
- 10.1111/j.1574-6968.2012.02565.x
- language
- English
- LU publication?
- yes
- id
- 86655bac-31d1-4d5e-a544-af86fecfbf1f (old id 2520042)
- date added to LUP
- 2016-04-01 09:49:34
- date last changed
- 2022-01-25 17:01:34
@article{86655bac-31d1-4d5e-a544-af86fecfbf1f, abstract = {{Deoxyribonucleoside kinases (dNKs) are essential in the mammalian cell but their 'importance' in bacteria, especially aquatic ones, is less clear. We studied two aquatic bacteria, Gram-negative Flavobacterium psychrophilum JIP02/86 and Polaribacter sp. MED152, for their ability to salvage deoxyribonucleosides (dNs). Both had a Gram-positive-type thymidine kinase (TK1), which could phosphorylate thymidine, and one non-TK1 dNK, which could efficiently phosphorylate deoxyadenosine and slightly also deoxycytosine. Surprisingly, the four tested dNKs could not phosphorylate deoxyguanosine, and apparently, these two bacteria are missing this activity. When tens of available aquatic bacteria genomes were examined for the presence of dNKs, a majority had at least a TK1-like gene, but several lacked any dNKs. Apparently, among aquatic bacteria, the role of the dN salvage varies.}}, author = {{Tinta, Tinkara and Slot Christiansen, Louise and Konrad, Anke and Liberles, David A and Turk, Valentina and Munch-Petersen, Birgitte and Piskur, Jure and Clausen, Anders Ranegaard}}, issn = {{1574-6968}}, keywords = {{aquatic bacteria; 3H-thymidine incorporation; deoxyribonucleoside kinase; nucleoside salvage}}, language = {{eng}}, number = {{2}}, pages = {{120--127}}, publisher = {{Oxford University Press}}, series = {{FEMS Microbiology Letters}}, title = {{Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosine.}}, url = {{http://dx.doi.org/10.1111/j.1574-6968.2012.02565.x}}, doi = {{10.1111/j.1574-6968.2012.02565.x}}, volume = {{331}}, year = {{2012}}, }